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The nematode worm C. elegans is widely used in basic and translational research. creation of transgenic strains by injecting DNA constructs into the worm's gonad an essential step many research projects. This paper describes fabrication use a minimalist microfluidic chip for performing microinjections. immobilized tight-fitting microchannel, one sidewall which thin elastomeric membrane through injection pipet penetrates to reach worm. neither broken nor clogged passing membrane, reseals when...
Abstract The nematode worm C. elegans is widely used in basic and translational research. creation of transgenic strains by injecting DNA constructs into the worm’s gonad an essential step many research projects. This paper describes fabrication use a minimalist microfluidic chip for performing microinjections. immobilized tight-fitting microchannel one sidewall which thin elastomeric membrane injection pipette penetrates to reach worm. neither broken nor clogged passing through membrane,...