A5066869670- Microtubule and mitosis dynamics
- Advanced Fluorescence Microscopy Techniques
- Cellular Mechanics and Interactions
- Protist diversity and phylogeny
- Photosynthetic Processes and Mechanisms
- Cell Image Analysis Techniques
- Optical Polarization and Ellipsometry
- Advanced X-ray Imaging Techniques
- Aortic Disease and Treatment Approaches
- Advanced Electron Microscopy Techniques and Applications
- Force Microscopy Techniques and Applications
- Animal Genetics and Reproduction
- Electron and X-Ray Spectroscopy Techniques
- Cephalopods and Marine Biology
- Reproductive Biology and Fertility
- Microfluidic and Bio-sensing Technologies
- Image Processing Techniques and Applications
- Color Science and Applications
- Advancements in Semiconductor Devices and Circuit Design
- Optical Coatings and Gratings
- Galectins and Cancer Biology
- Digital Holography and Microscopy
- Nanowire Synthesis and Applications
- Cardiomyopathy and Myosin Studies
- Near-Field Optical Microscopy
Kanazawa Medical University
2016-2025
Inoue Hospital
2024-2025
Dokkyo Medical University Saitama Medical Center
2020-2025
Social Insurance Saitama Chuo Hospital
2020-2025
Tokyo Medical University
2021-2023
Suita Municipal Hospital
2013-2023
Hokkaido University
2009-2021
Kawasaki Municipal Hospital
2021
Kawasaki Hospital
2021
Kyushu University
2019-2020
This article summarizes our current views on the dynamic structure of mitotic spindle and its relation to chromosome movements. The following statements are based measurements birefringence fibers in living cells, normally developing or experimentally modified by various physical chemical agents, including high low temperatures, antimitotic drugs, heavy water, ultraviolet microbeam irradiation. Data were also obtained concomitantly with electron microscopy employing a new fixative through...
The correct positioning of the nucleus is often important in defining spatial organization cell, for example, determining cell division plane. In interphase Schizosaccharomyces pombe cells, positioned middle cylindrical an active microtubule (MT)-dependent process. Here, we used green fluorescent protein markers to examine dynamics MTs, spindle pole body, and nuclear envelope living cells. We find that MTs are organized three four antiparallel MT bundles arranged along long axis with plus...
The core fucosylation (alpha1,6-fucosylation) of glycoproteins is widely distributed in mammalian tissues, and altered under pathological conditions. To investigate physiological functions the fucose, we generated alpha1,6-fucosyltransferase (Fut8)-null mice found that disruption Fut8 induces severe growth retardation death during postnatal development. Histopathological analysis revealed Fut8(-/-) showed emphysema-like changes lung, verified by a compliance analysis. Biochemical studies...
Video cameras with contrast and black level controls can yield polarized light differential interference microscope images unprecedented image quality, resolution, recording speed. The theoretical basis practical aspects of video polarization microscopy are discussed several applications in cell biology illustrated. These include: birefringence cortical structures beating cilia Stentor, rotating flagella on a single bacterium, growth morphogenesis echinoderm skeletal spicules culture,...
Urinary stones (urolithiasis) have been categorized as kidney (renal calculus), ureteric (ureteral calculus and ureterolith), bladder (bladder urethral (urethral calculus); however, the mechanisms underlying their promotion related injuries in glomerular tubular cells remain unclear. Although lifestyle-related diseases (LSRDs) such hyperglycemia, type 2 diabetic mellitus, non-alcoholic fatty liver disease/non-alcoholic steatohepatitis, cardiovascular disease are risk factors for...
Meiosis I metaphase spindles were isolated from oocytes of the sea-star Pisaster ochraceus by a method that produced no detectable net loss in spindle birefringence. Some fixed immediately and embedded sectioned for electron microscopy. Others laminated between gelatine pellicles perfusion chamber, then sequentially reversibly imbibed with series media increasing refractive indices. Electron microscopy showed little else besides microtubules isolates, other component present could account...
In the polarizing microscope set for extinction, only that image whose polarization has been altered is available to form an image. The lenses themselves introduce such alternation by rotation of plane rays having oblique incidence. This paper shows diffraction a pinhole sin2θ·J3(r)/r, where θ and r are polar coordinates in plane. four bright zones separated dark cross central pattern becomes four-leaf clover form. point source through plate uniaxial crystal cut perpendicular its optic axis...
The molecular basis of microtubule dynamic instability is controversial, but thought to be related a "GTP cap." A key prediction the GTP cap model that proposed labile GDP-tubulin core will rapidly dissociate if GTP-tubulin lost. We have tested this by using UV microbeam cut ends from elongating microtubules. Phosphocellulose-purified tubulin was assembled onto plus and minus sea urchin flagellar axoneme fragments at 21-22 degrees C. assembly dynamics individual microtubules were recorded in...
ABSTRACT Using light microscopy techniques, we have studied the movements that follow fertilization in denuded egg of ascidian Phallusia mammillata. In particular, our observations show that, as a result series described below, mitochondria-rich subcortical myoplasm is split two parts during second phase ooplasmic segregation. This offers potential explanation for origin larval muscle cells from both posterior and anterior blastomeres. The first visible event at bulging animal pole egg,...
A pair of pluteus skeletal spicules arises from a calcareous granules via the triradiate form. In polarized light, each spicule behaves as though carved out single crystal magnesian calcite. The optic axis lies perpendicular to plane and parallel body rod pluteus. However, in scanning electron microscope, surface appeared smooth or somewhat spongy manifested no faces. Neither etching nor fracturing revealed underlying crystalline texture. Nevertheless, rhombohedral calcite crystals could be...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTHandbook of Chemical Microscopy.Shinya InouéCite this: J. Am. Chem. Soc. 1959, 81, 15, 4121Publication Date (Print):August 1, 1959Publication History Published online1 May 2002Published inissue 1 August 1959https://pubs.acs.org/doi/10.1021/ja01524a091https://doi.org/10.1021/ja01524a091research-articleACS PublicationsRequest reuse permissionsArticle Views48Altmetric-Citations-LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum full...