A5032185455- Seismic Imaging and Inversion Techniques
- Glycosylation and Glycoproteins Research
- Seismic Waves and Analysis
- Methane Hydrates and Related Phenomena
- Galectins and Cancer Biology
- Hydrocarbon exploration and reservoir analysis
- Hydraulic Fracturing and Reservoir Analysis
- Ubiquitin and proteasome pathways
- Geological and Geophysical Studies
- TGF-β signaling in diseases
- Geophysical Methods and Applications
- Monoclonal and Polyclonal Antibodies Research
- Underwater Acoustics Research
- Land Use and Ecosystem Services
- Histone Deacetylase Inhibitors Research
- 14-3-3 protein interactions
- Enhanced Oil Recovery Techniques
- Reservoir Engineering and Simulation Methods
- Carbohydrate Chemistry and Synthesis
- Image and Signal Denoising Methods
- Drilling and Well Engineering
- Geological Modeling and Analysis
- Pharmacological Effects and Toxicity Studies
- Transgenic Plants and Applications
- Advanced Computational Techniques and Applications
China University of Geosciences (Beijing)
2015-2024
China University of Petroleum, Beijing
2009-2023
Beijing Urban Construction Design & Development Group (China)
2019-2022
National Institute of Allergy and Infectious Diseases
2019-2021
National Institutes of Health
2013-2021
Johns Hopkins University
2013-2019
Johns Hopkins Medicine
2014-2019
National Cancer Institute
2008-2011
Center for Cancer Research
2009-2011
Osaka University
2005-2007
The core fucosylation (alpha1,6-fucosylation) of glycoproteins is widely distributed in mammalian tissues, and altered under pathological conditions. To investigate physiological functions the fucose, we generated alpha1,6-fucosyltransferase (Fut8)-null mice found that disruption Fut8 induces severe growth retardation death during postnatal development. Histopathological analysis revealed Fut8(-/-) showed emphysema-like changes lung, verified by a compliance analysis. Biochemical studies...
alpha1,6-Fucosyltransferase (Fut8) catalyzes the transfer of a fucose residue to N-linked oligosaccharides on glycoproteins via an alpha1,6-linkage form core fucosylation in mammals. We recently found that disruption Fut8 gene induces severe growth retardation and early postnatal death. To investigate molecular mechanism involved, we have established embryonic fibroblasts Fut8+/+ Fut8-/-, derived from wild-type Fut8-null mice, respectively. Interestingly, epidermal factor (EGF)-induced...
The α1,6-fucosyl residue (core fucose) of glycoproteins is widely distributed in mammalian tissues and altered under pathological conditions. A probe that specifically detects core fucose important for understanding the role this oligosaccharide structure. Aleuria aurantia lectin (AAL) Lens culimaris agglutinin-A (LCA) have been often used as carbohydrate probes glycoproteins. Here we show, by using surface plasmon resonance (SPR) analysis, Aspergillus oryzae l-fucose-specific (AOL) has...
Prostate cancer is the most common among men in U.S. and worldwide, androgen-deprivation therapy remains principal treatment for patients. Although a majority of patients initially respond to therapy, will eventually develop castration resistance. An increased understanding mechanisms that underline pathogenesis resistance therefore needed novel therapeutics. LNCaP PC3 prostate cell lines are models androgen-dependence androgen-independence, respectively. Herein, we report comparative...
Aberrant protein glycosylation is known to be associated with the development of cancers. The aberrant glycans are produced by combined actions changed enzymes, substrates and transporters in synthesis pathways cancer cells. To identify enzymes aggressive prostate (PCa), we analyzed difference expression glycosyltransferase genes between non-aggressive PCa. Three candidate encoding glycosyltransferases that were elevated PCa subsequently selected. three candidates was then further evaluated...
Mammalian alpha1,6-fucosyltransferase (FUT8) catalyses the transfer of a fucose residue from donor substrate, guanosine 5'-diphosphate-beta-L-fucose to reducing terminal N-acetylglucosamine (GlcNAc) core structure an asparagine-linked oligosaccharide. Alpha1,6-fucosylation, also referred as fucosylation, plays essential role in various pathophysiological events. Our group reported that FUT8 null mice showed severe growth retardation and emphysema-like lung-destruction result dysfunction...
Aberrant glycosylation is associated with most of the diseases. Direct imaging and profiling N-glycans on tissue sections can reveal tissue-specific and/or disease-associated N-glycans, which not only could serve as molecular signatures for diagnosis but also shed light functional roles these biomolecules. Mass spectrometry (MSI) a powerful tool that has been used to correlate peptides, proteins, lipids, metabolites their underlying histopathology in sections. Here, we report an MSI...
Abstract Controlled protein degradation mediated by ubiquitin/proteasome system (UPS) plays a crucial role in modulating broad range of cellular responses. Dysregulation the UPS often accompanies tumorigenesis and progression. Here, we report that Smad ubiquitination regulatory factor 2 (Smurf2), HECT-domain containing E3 ubiquitin ligase, is up-regulated certain breast cancer tissues cells. We show reduction Smurf2 expression with specific short interfering RNA metastatic cells induces cell...
Smad ubiquitin regulatory factors (Smurfs) are HECT-domain E3 ligases that regulate diverse cellular processes, including normal and tumor cell migration. However, the underlying mechanism of Smurfs' role in migration is not fully understood. Here we show Smurf1 induces ubiquitination necrosis factor receptor-associated 4 (TRAF4) at K190. Using K190R mutant TRAF4, demonstrate Smurf1-induced required for proper localization TRAF4 to tight junctions confluent epithelial cells. We further...
Fuzzy-ball fluid is a two-phase composed of fuzzy-ball and base fluid. It can enhance oil recovery by approximately 30% after water flooding polymer flooding, which has been demonstrated in laboratory experiments field applications. However, the EOR mechanism still needs further research. In this study, microstructure was observed using optical cryo-scanning electron microscopy. The investigated microscopic glass etching model experiment inter-molecular force calculation. contribution ratio...
Changes in oligosaccharide structures are associated with numerous physiological and pathological events. In this study, the effects of cell-cell interactions on N-linked oligosaccharides (N-glycans) were investigated GE11 epithelial cells. N-glycans purified from whole cell lysates by hydrazinolysis then detected high performance liquid chromatography mass spectrometry. Interestingly, population bisecting GlcNAc-containing N-glycans, formation which is catalyzed...
Journal Article Loss of Core Fucosylation Low-Density Lipoprotein Receptor–Related Protein-1 Impairs Its Function, Leading to the Upregulation Serum Levels Insulin-Like Growth Factor–Binding Protein 3 in Fut8−/− Mice Get access Seung Ho Lee, Lee †To whom correspondence should be addressed. Tel: +81-6-6879-3421, Fax: +81-6-6879-3429, E-mail: proftani@biochem.med.osaka-u.ac.jp Search for other works by this author on: Oxford Academic PubMed Google Scholar Motoko Takahashi, Takahashi Koichi...
More than 90% of the glycolipid in mammalian testis consists a unique sulfated glyceroglycolipid, seminolipid. The sulfation molecule is catalyzed by Golgi membrane-associated sulfotransferase, cerebroside sulfotransferase (CST). Disruption Cst gene mice results male infertility due to arrest spermatogenesis prior metaphase first meiosis. However, issue which side cell function—germ cells or Sertoli cells—is deteriorated this mutant mouse remains unknown. Our findings show that defect germ...
Alpha1,6-fucosyltransferase (Fut8) plays important roles in physiological and pathological conditions. Fut8-deficient (Fut8−/−) mice exhibit growth retardation, earlier postnatal death, emphysema-like phenotype. To investigate the underlying molecular mechanism by which retardation occurs, we examined mRNA expression levels of Fut8−/− embryos (18.5 days postcoitum [dpc]) using a cDNA microarray. The DNA microarray real-time polymerase chain reaction (PCR) analysis showed that group genes,...
With social media platforms becoming popular in recent years, Big Data has become an effective way to explore human-oriented service capability of parks. In this research, the spatial accessibility urban parks under four different travel modes (driving, transiting, biking, and walking) multiple was analyzed by integrating actual experiences visitors real time. We took built-up area Chengdu, Sichuan Province, China as research drew conclusions follows: 1) park Chengdu still a large space for...
Lung cancer is the number one cause of cancer-related deaths in United States and worldwide. The complex protein changes and/or signature expression lung cancer, particularly non-small cell (NSCLC) has not been well defined. Although several studies have investigated profile cancers, knowledge far from complete. Among early studies, mucin5B (MUC5B) suggested to play an important role tumor progression. MUC5B major gel-forming mucin airway. In this study, we overall adenocarcinomas, most...
Bioretention systems for collecting, filtering, and treating pollutants (e.g., heavy metals) in stormwater runoff have recently attracted increased attention from the scientific community. However, excess metal accumulation after long-term operation may deteriorate effluent quality, affect plant growth landscape benefits, shorten service life of bioretention systems. It has been shown that most metals accumulated were distributed soil/media layer, their concentration decreased with...