A5091783753- Autophagy in Disease and Therapy
- Cellular transport and secretion
- Antibiotics Pharmacokinetics and Efficacy
- bioluminescence and chemiluminescence research
- Innovative Microfluidic and Catalytic Techniques Innovation
- RNA Interference and Gene Delivery
- Calcium signaling and nucleotide metabolism
- Microfluidic and Capillary Electrophoresis Applications
- Lipid Membrane Structure and Behavior
- Endoplasmic Reticulum Stress and Disease
- Advanced Biosensing Techniques and Applications
- Genomic variations and chromosomal abnormalities
- Advanced Fluorescence Microscopy Techniques
- Erythrocyte Function and Pathophysiology
- Photosynthetic Processes and Mechanisms
- Biosensors and Analytical Detection
- Bacterial Genetics and Biotechnology
- Genomics and Rare Diseases
- Cancer Research and Treatments
- Cancer Genomics and Diagnostics
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Biotin and Related Studies
- Origins and Evolution of Life
- Photoreceptor and optogenetics research
- Electrochemical sensors and biosensors
Tempus Labs (United States)
2024-2025
Vanderbilt University Medical Center
2014-2016
Vanderbilt University
2010-2016
Harvard University
2015-2016
Nashville Oncology Associates
2014
Kennesaw State University
2008
Fluorescence recovery after photobleaching (FRAP) is a powerful, versatile, and widely accessible tool to monitor molecular dynamics in living cells that can be performed using modern confocal microscopes. Although the basic principles of FRAP are simple, quantitative analysis requires careful experimental design, data collection, analysis. In this unit, we discuss theoretical basis for FRAP, followed by step-by-step protocols acquisition laser-scanning microscope (1) measuring diffusion...
How the plasma membrane is bent to accommodate clathrin-independent endocytosis remains uncertain. Recent studies suggest Shiga and cholera toxin induce curvature required for their uptake into carriers by binding cross-linking multiple copies of glycosphingolipid receptors on membrane. But it unclear if toxin-induced sphingolipid crosslinking provides sufficient mechanical force deforming membrane, or host cell factors also contribute this process. To test this, we imaged B-subunit...
MAP1LC3B (microtubule-associated protein 1 light chain 3, LC3) is a key component of the autophagy pathway, contributing to both cargo selection and autophagosome formation in cytoplasm. Emerging evidence suggests that nuclear forms LC3 are also functionally important; however, mechanisms facilitate targeting trafficking between nucleus cytoplasm under steady-state conditions poorly understood. In this study, we examine how residues known regulate interactions other proteins or RNA (F52 L53,...
Whole-genome sequencing (WGS) is increasingly preferred for clinical applications due to its comprehensive coverage, effectiveness in detecting copy number variants (CNVs), and declining costs. However, systematic evaluations of WGS CNV callers tailored germline testing-where high sensitivity confirmation reported CNVs are essential-remain necessary. Clinical reporting typically emphasizes affecting coding regions over precise breakpoint detection. This study benchmarks several short-read...
Abstract Identification of NCCN and WHO guideline-indicated genetic variation is crucial for diagnosis, risk assessment, therapeutic decision-making in patients with myeloid malignancies. Current practice often utilizes small mutation panels, cytogenetics (CG), and/or fluorescence situ hybridization—independent tests that require a separate specimen have timeline performance limitations. Whole-genome sequencing (WGS) promising alternative rapid turnaround times low sample requirements, but...
Mutations and alterations in caveolin-1 expression levels have been linked to a number of human diseases. How misregulation contributes disease is not fully understood, but has proposed involve the intracellular accumulation mutant forms protein. To better understand molecular basis for trafficking defects that trap intracellularly, we compared properties GFP-tagged version P132L, form previously breast cancer, with wild-type caveolin-1. Unexpectedly, caveolin-1-GFP also accumulated leading...
MAP1LC3B, an ortholog of yeast Atg8 and a member the family proteins formerly also known as ATG8 in mammals (LC3B henceforth text), functions autophagosome formation autophagy substrate recruitment. LC3 exists both soluble (autophagosome-independent) form well lipid modified that becomes tightly incorporated into autophagosomal membranes. Although is to associate with tens proteins, relatively little about aside from its interactions conjugation machinery. In previous studies we found...
Multianalyte microphysiometry, a real-time instrument for simultaneous measurement of metabolic analytes in microfluidic environment, was used to explore the effects cholera toxin (CTx). Upon exposure CTx PC-12 cells, anaerobic respiration triggered, measured as increases acid and lactate production decrease oxygen uptake. We believe responses observed are due CTx-induced activation adenylate cyclase, increasing cAMP resulting switch respiration. Inhibitors (H-89, brefeldin A) stimulators...
The protein microtubule-associated 1, light chain 3 (LC3) functions in autophagosome formation and plays a central role the autophagy pathway. Previously, we found LC3 diffuses more slowly cells than is expected for freely diffusing monomer, suggesting it may constitutively associate with macromolecular complex containing other components of In current study, used Förster resonance energy transfer (FRET) microscopy fluorescence recovery after photobleaching (FRAP) to investigate interactions...
Selective macroautophagy/autophagy—with the help of molecular receptors—captures cargo for lysosomal degradation. Among best-studied receptors is SQSTM1/p62, a homo-oligomeric ubiquitin binding protein, which binds to both and MAP1LC3B/LC3, protein important autophagosome biogenesis. Although mechanisms underlying interaction LC3 SQSTM1 have been extensively studied, very little known about size or organization soluble complexes formed between prior phagophore (the precursor) in live cells...
Abstract Whole-genome sequencing (WGS) is increasingly favored over other genomic methods for clinical applications due to its comprehensive coverage and declining costs. WGS particularly useful the detection of copy number variants (CNVs), presumed be more accurate than targeted assays such as WES or gene panels, because it can identify breakpoints in addition changes depth. Recent advancements bioinformatics tools, including those employing hardware acceleration machine learning, have...
Extended abstract of a paper presented at Microscopy and Microanalysis 2011 in Nashville, Tennessee, USA, August 7–August 11, 2011.