Preterm birth (PTB; <37 weeks of gestation) impacts ∼11% all pregnancies and contributes to 1 million neonatal deaths worldwide annually.

Abstract Immature B cells in the bone marrow emigrate into spleen during adult lymphopoiesis. Here, we report that emigration is shifted to earlier B-cell stages mice with orthotopic breast cancer, spontaneous ovarian and possibly human carcinoma. Using mouse aspirates models challenged highly metastatic 4T1 cancer cells, demonstrated this was result of secretion thymic stromal lymphopoietin (TSLP) by cells. First, TSLP downregulated surface expression (BM) retention receptors CXCR4 VLA4...

Background Microbial invasion of the intraamniotic cavity and inflammation are factors associated with spontaneous preterm birth. Understanding route kinetics infection, sites colonization, mechanisms host inflammatory response is critical to reducing birth risk. Objectives This study developed an animal model ascending infection live bacteria ( E . coli ) in pregnant CD-1 mice goal better understanding process microbial inflammation. Study design Multiple experiments were conducted this...

The DNA of all living organisms is persistently damaged by endogenous reactions including deamination and oxidation. Such damage, if not repaired correctly, can result in mutations that drive tumor development. In addition to chemical recent studies have established bases be enzymatically modified, generating many the same modified bases. Irrespective mechanism formation, alter DNA-protein interactions therefore modulate epigenetic control gene transcription. simultaneous presence both...

Oxidative stress (OS) induced activation of p38 mitogen-activated kinase (MAPK) and cell fate from signaling was tested using the human fetal membrane's amnion epithelial cells (AEC). We created KO AEC CRISPR/Cas9 approach in response to OS on an AEC-free membrane extracellular matrix (ECM). Screening image CyTOF indicated causing epithelial-mesenchymal transition (EMT). Further testing revealed deficiency prevented senescence, EMT, migration, inflammation. To functionally validate vitro...

Abstract DNA damage drives genetic mutations that underlie the development of cancer in humans. Multiple pathways have been described mammalian cells which can repair this damage. However, most work to date has focused upon single lesions DNA. We present here a combinatorial system allows assembly duplexes containing or multiple types by ligating together six oligonucleotides damaged modified bases. The dual fluorescent labels allowing examination both strands simultaneously, order study...

This study investigated the efficacy of Omega-7 isolated from sea buckthorn oil (Polyvit Co., Ltd, Gangar Holding, Ulaanbaatar, Mongolia) in ovine burn wound healing models. In vitro, proliferation (colony-forming rate) and migration (scratch) assays using cultured primary keratinocytes were performed with or without 0.025% 0.08% Omega-7, respectively. The colony-forming rate group at 72 96 h significantly higher than control (P < 0.05). percentage closure scratch assay was 17 vivo, 4%...

The lack of a clinically relevant animal models for research in facial nerve reconstruction is challenging. In this study, we investigated the surgical anatomy ovine sural as potential candidate reconstruction, and performed its histological quantitative analysis comparison to buccal branch (BB) using cadaver anesthetized sheep. descended lower leg along short saphenous vein. length was 14.3 ± 0.5 cm. distance from posterior edge lateral malleolus 7.8 1.8 mm. mean number myelinated fibers...

The hydrolytic deamination of cytosine and 5-methylcytosine drives many the transition mutations observed in human cancer. deamination-induced mutagenic intermediates include either uracil or thymine adducts mispaired with guanine. While a substantial array methods exist to measure other types DNA adducts, pose unusual analytical problems, adequate them have not yet been developed. We describe here novel hybrid glycosylase (TDG) that is comprised 29-amino acid sequence from TDG linked...

We investigated the role of endothelial glycocalyx and its component syndecan-1 protein in pathophysiology sepsis-induced vascular hyper-permeability examined therapeutic effects high-molecular-weight sodium hyaluronate (HMW-SH). Methods Sepsis was induced by cotton smoke inhalation followed intranasal administration Pseudomonas aeruginosa female (&gt; 6 months) Balb/c knockout mice. Survival mice, lung capillary integrity, water content, were determined with or without HMW-SH treatment...

Abstract Background Sepsis is associated with a highest mortality rate in the ICU. Present study tests efficacy of combined therapy vitamin C, hydrocortisone and thiamine (combined therapy) ovine model sepsis induced by Pseudomonas aeruginosa . In this study, was sheep instillation (1 × 10 11 CFU) into lungs via bronchoscope, under anesthesia. Nine hours after injury, intravenous infusion C (0.75 g every 6 h), (25 mg (100 12 h) or saline given to treatment control groups. Cardiopulmonary...

Recently, we constructed a hybrid thymine DNA glycosylase (hyTDG) by linking 29-amino acid sequence from the human with catalytic domain of mismatch (MIG) M. thermoautotrophicum, increasing overall activity glycosylase. Previously, it was shown that tyrosine to lysine (Y126K) mutation in site MIG could convert lyase activity. We made corresponding our hyTDG create hyTDG-lyase (Y163K). Here, report mutant has robust activity, over broad temperature range, and is active under multiple buffer...

Abstract Glioblastoma is a heterogenous tumor comprised of multiple molecular subtypes cancer cells and stem-like cells. Of particular importance are the cell niche population as they believed to be more resistant existing therapies re-seed following initial response standard-of-care treatment. To better understand glioblastoma we completed an untargeted proteomic analysis 35 patient derived stem lines. This revealed increase in several important proteins involved uridine salvage pathway,...

Abstract Glioblastoma is a heterogenous tumor comprised of multiple molecular subtypes cancer cells and stem-like cells. Of particular importance are the cell niche population as they believed to be more resistant existing therapies re-seed following initial response standard-of-care treatment. To better understand glioblastoma we completed an untargeted proteomic analysisof 35 patient derived stem lines. This system driven analysis revealed increase in several important proteins,...

Abstract Temozolomide is the only FDA-approved drug that has demonstrated improved overall survival in patients with Glioblastoma. Despite clinical impact it had, today largely remains less than two years current standard-of-care. a prodrug undergoes hydrolysis releasing 5-amino-4-imidazole carboxamide (AIC) before can alkylate DNA. The DNA damage response invoked by TMZ depletes NAD+ levels. We present evidence from mass spectrometry LC-MS/MS studies AIC released be aberrantly incorporated...

Delayed re-epithelialization of grafted and donor site wounds burn patient is the challenging problem. The cell proliferation in wound negatively affected by many factors. Omega-7 (ω-7) a most abundant fatty acid sea buckthorn seed oil that has been shown to be critically involved skin mucosal tissue regeneration. We have previously reported promoted healing sheep. aim this study was test hypothesis topical application ω-7 promotes via increasing telomerase activity wounds. In vitro, scratch...

ABSTRACT In preclinical studies, the protective effects of female sex hormones and immunosuppressive male were demonstrated. However, gender-related differences in multiorgan failure mortality clinical trials have not been consistently explained. This study aims to investigate development progression sepsis using a clinically relevant ovine model sepsis. Adult Merino (n=7) sheep surgically prepared with multiple catheters before study. To induce sepsis, bronchoscopy instilled...

&lt;div&gt;Abstract&lt;p&gt;Immature B cells in the bone marrow emigrate into spleen during adult lymphopoiesis. Here, we report that emigration is shifted to earlier B-cell stages mice with orthotopic breast cancer, spontaneous ovarian and possibly human carcinoma. Using mouse aspirates models challenged highly metastatic 4T1 cancer cells, demonstrated this was result of secretion thymic stromal lymphopoietin (TSLP) by cells. First, TSLP downregulated surface expression (BM) retention...

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&lt;div&gt;Abstract&lt;p&gt;Immature B cells in the bone marrow emigrate into spleen during adult lymphopoiesis. Here, we report that emigration is shifted to earlier B-cell stages mice with orthotopic breast cancer, spontaneous ovarian and possibly human carcinoma. Using mouse aspirates models challenged highly metastatic 4T1 cancer cells, demonstrated this was result of secretion thymic stromal lymphopoietin (TSLP) by cells. First, TSLP downregulated surface expression (BM) retention...