A5073849250- Plant Pathogens and Fungal Diseases
- Probiotics and Fermented Foods
- Mycotoxins in Agriculture and Food
- Mycorrhizal Fungi and Plant Interactions
- Gut microbiota and health
- RNA Research and Splicing
- Plant Virus Research Studies
- Fermentation and Sensory Analysis
- Plant and Fungal Interactions Research
- Plant-Microbe Interactions and Immunity
- RNA and protein synthesis mechanisms
- Fungal Biology and Applications
- Horticultural and Viticultural Research
- Plant Reproductive Biology
- Legume Nitrogen Fixing Symbiosis
- Yeasts and Rust Fungi Studies
- Plant pathogens and resistance mechanisms
- Microbial Metabolism and Applications
- Identification and Quantification in Food
- Insect-Plant Interactions and Control
- Plant and fungal interactions
- Plant Pathogenic Bacteria Studies
- Microbial Metabolites in Food Biotechnology
- Plant Parasitism and Resistance
- Protein Hydrolysis and Bioactive Peptides
Agricultural Biotechnology Institute
1999-2019
Hungarian Meat Research Institute
2018
National Agricultural Research and Innovation Centre
2016
Corvinus University of Budapest
2013
Budapest Institute
2013
Magyar Agrár- és Élettudományi Egyetem
2006
Ministry of Agriculture
2002-2005
Ministry of Agriculture and Rural Development
2000
Hungarian Academy of Sciences
1996
Plant Protection Institute
1996
In plants, RNA silencing (RNA interference) is an efficient antiviral system, and therefore successful virus infection requires suppression of silencing. Although many viral suppressors have been identified, the molecular basis poorly understood. It proposed that various inhibit by targeting different steps. However, as double-stranded RNAs (dsRNAs) play key roles in silencing, it was speculated dsRNA binding might be a general strategy. Indeed, shown related aureusvirus P14 tombusvirus P19...
Nonsense-mediated mRNA decay (NMD) is a eukaryotic quality control mechanism that identifies and eliminates aberrant mRNAs containing premature termination codon (PTC). Although, key trans-acting NMD factors, UPF1, UPF2 UPF3 are conserved in yeast mammals, the cis-acting elements different. In yeast, short specific sequences or long 3′-untranslated regions (3′-UTRs) render an subject to NMD, while mammals' 3′-UTR located introns trigger NMD. Plants also possess system, although little known...
RNA silencing is a conserved eukaryotic gene regulatory system in which sequence specificity determined by small RNAs. Plant also acts as an antiviral mechanism; therefore, viral infection requires expression of suppressor. The mechanism and the evolution suppression are still poorly understood. Tombusvirus open reading frame (ORF) 5-encoded P19 size-selective double-stranded (dsRNA) binding protein that suppresses sequestering interfering RNAs (siRNAs), determinant system. To better...
ABSTRACT To assess the potential for mating in several Fusarium species with no known sexual stage, we developed degenerate and semidegenerate oligonucleotide primers to identify conserved type ( MAT ) sequences these fungi. The putative α high-mobility-group (HMG) box from avenaceum , F. culmorum poae semitectum were compared similar that described previously other members of genus. DNA regions flanking amplified obtained by inverse PCR. These data used develop diagnostic suitable clear...
ABSTRACT Mating type in the Gibberella fujikuroi species complex is controlled by a single locus with two alleles and usually identified following sexual crosses standard, female-fertile tester isolates. The mating have been arbitrarily designated “+” “−” within each biological species, nomenclature tied to standard strains. We developed pair of PCR primers that can be used amplify unique fragment one ( MAT-2 ) from at least seven this complex. Based on amplification pattern, we propose...
Nonsense-mediated mRNA decay (NMD) is a eukaryotic quality control system that identifies and degrades mRNAs containing premature termination codons (PTCs). If translation terminates at PTC, the UPF1 NMD factor binds terminating ribosome recruits UPF2 UPF3 to form functional complex, which triggers rapid of PTC-containing transcript. Although deficiency seedling lethal in plants, mechanism plant remains poorly understood. To understand how formation complex leads transcript we functionally...
Abstract Lactic acid bacteria (LAB) in the microbiota play an important role human and animal health and, when used as probiotics, can contribute to increased growth performance livestock management. Animals living their native habitat serve natural sources of microorganisms, so isolation LAB strains from wild boars could provide opportunity develop effective probiotics improve production swine industry. In this study, probiotic potential 56 isolates, originated ileum, colon, caecum faeces 5...
During sclerotial infection of Sclerotinia sclerotiorum the mycoparasite Coniothyrium minitans penetrates through host cell wall, which contains beta-1,3-glucan as its major component. A PCR-based strategy was used to clone a beta-1,3-glucanase-encoding gene, designated cmg1, from cDNA library fungus. The nucleotide and deduced amino acid sequences this gene showed high levels similarity other fungal exo-beta-1,3-glucanase genes. calculated molecular mass protein (without predicted...
When a ribosome reaches stop codon, the eukaryotic Release Factor 1 (eRF1) binds to A site of and terminates translation. In yeasts plants, both over- underexpression eRF1 lead altered phenotype indicating that expression should be strictly controlled. However, regulation level is still poorly understood. Here we show plant controlled by complex negative autoregulatory circuit, which based on unique features 3΄untranslated region (3΄UTR) eRF1-1 transcript. The codon mRNA in translational...
The present work compares volatile aroma components identified in dry base wines, young Aszú wines and noble rotted (aszú) grape berries from Tokaj wine district Hungary. Volatile were determined by GC-MS, for sample preparation liquid-liquid extraction was used. greatest differences between the of found monoterpene alcohols, hydroxy-, oxo- dicarboxylic acid esters lactones. contained lower relative amounts terpene while ester lactone concentrations higher. Some lactones detected only...
Certain apple cultivars accumulate to high levels in their nectar and stigma exudate an acidic chitinase III protein that can protect against pathogens including fire blight disease causing Erwinia amylovora. To prevent microbial infections, flower nectars exudates contain various antimicrobial compounds. amylovora, the bacterium of devastating disease, is model pathogen multiplies secretions infects through nectaries. Although Erwinia-resistant apples are not available, certain tolerant. It...
Vegetative compatibility tests and random amplification of polymorphic DNA (RAPD) were used to assess genetic relationships amongst 54 strains Fusarium poae obtained from various geographical regions. Twenty‐seven assigned eight multiple member vegetative groups (VCGs), while the other 27 isolates found form single‐member VCGs. There was a partial correlation between VCG origin, but relationship not always clear. However, no observed host plant origin. RAPD patterns closely associated with...
For two fungal strains to be vegetatively compatible and capable of forming a stable vegetative heterokaryon they must carry matching alleles at series loci variously termed het or vic genes. Cloned het/vic genes from Neurospora crassa Podospora anserina have no obvious functional similarity various cellular functions. Our objective was identify the homologue het-c gene in Fusarium proliferatum determine if this has compatibility function economically important widely dispersed pathogen. In...