A5012134465- Plant Virus Research Studies
- Plant-Microbe Interactions and Immunity
- Insect-Plant Interactions and Control
- Plant and Fungal Interactions Research
- Plant tissue culture and regeneration
- Plant Pathogenic Bacteria Studies
- Plant Disease Resistance and Genetics
- Bacteriophages and microbial interactions
- Plant Pathogens and Resistance
- Insect Resistance and Genetics
- Transgenic Plants and Applications
- Plant Molecular Biology Research
- Plant Parasitism and Resistance
- Insect Pest Control Strategies
- Research on scale insects
- Legume Nitrogen Fixing Symbiosis
- RNA Research and Splicing
- Potato Plant Research
- Biosensors and Analytical Detection
- Plant Stress Responses and Tolerance
- Agricultural pest management studies
- Herpesvirus Infections and Treatments
- Plant Genetic and Mutation Studies
- Islanding Detection in Power Systems
- Plant nutrient uptake and metabolism
Scottish Agricultural Science Agency
2009-2022
James Hutton Institute
2012
University of Edinburgh
2004-2012
Czech Agrifood Research Center
1998-2010
Rutgers, The State University of New Jersey
2009
Sainsbury Laboratory
2005
John Innes Centre
2005
Laboratoire des Interactions Plantes Micro-Organismes
1995-2001
Centre National de la Recherche Scientifique
1996-2001
Abstract Powdery mildew fungi are obligate biotrophic pathogens that only grow on living hosts and cause damage in thousands of plant species. Despite their agronomical importance, little direct functional evidence for genes pathogenicity virulence is currently available because mutagenesis transformation protocols lacking. Here, we show the accumulation barley (Hordeum vulgare) wheat (Triticum aestivum) double-stranded or antisense RNA targeting fungal transcripts affects development...
Bax, a death-promoting member of the Bcl-2 family proteins, triggered cell death when expressed in plants from tobacco mosaic virus vector. Analysis Bax deletion mutants demonstrated requirement for BH1 and BH3 domains promoting rapid death, whereas carboxyl-terminal transmembrane domain completely abolished lethality plants. The phenotype induced by closely resembled hypersensitive response wild-type carrying N gene. function correlated with accumulation defense-related protein PR1,...
RNA silencing is conserved in a broad range of eukaryotes and includes the phenomena interference animals posttranscriptional gene (PTGS) plants. In plants, PTGS acts as an antiviral system; successful virus infection requires suppression or evasion induced response. Small interfering RNAs (siRNAs) accumulate plants infected with positive-strand viruses provide specificity to this RNA-mediated defense. We present here results survey virus-specific siRNAs characterized by sequence analysis...
Abstract We successfully implemented virus-induced gene silencing (VIGS) in barley (Hordeum vulgare) for the functional characterization of genes required Mla13-mediated resistance toward biotrophic pathogen Blumeria graminis f. sp. hordei. Initially, cultivars were screened their ability to host stripe mosaic virus (BSMV)-VIGS vector by allowing its replication and systemic movement without causing excessive symptoms. Phytoene desaturase leading photobleaching was used as a phenotypic...
Nearly 7000 Arabidopsis thaliana ‐expressed sequence tags (ESTs) from 10 cDNA libraries have been sequenced, of which almost 5000 non‐redundant submitted to the EMBL data bank. The quality used is analysed. Similarity searches in international protein banks allowed detection significant similarities a wide range proteins many organisms. Alignment with ESTs rice systematic sequencing project has amino acid motifs are conserved between two organisms, thus identifying genes encoding highly...
Summary A diverse range of plant proteases are implicated in pathogen perception and subsequent signalling execution disease resistance. We demonstrate, using protease inhibitors virus‐induced gene silencing (VIGS), that the papain cysteine cathepsin B is required for resistance hypersensitive response (HR). VIGS prevented programmed cell death (PCD) compromised induced by two distinct non‐host bacterial pathogens. It also suppressed HR triggered transient co‐expression potato R3a...
Recent advances in high-throughput sequencing technologies and bioinformatics have generated huge new opportunities for discovering diagnosing plant viruses viroids. Plant virology has undoubtedly benefited from these methodologies, but at the same time, faces now substantial bottlenecks, namely biological characterization of newly discovered analysis their impact biosecurity, commercial, regulatory, scientific levels. This paper proposes a scaled progressive framework efficient risk...
Potato virus X (PVX) requires three virally encoded proteins, the triple gene block (TGB), for movement between cells. TGB1 is a multifunctional protein that suppresses host silencing and moves from cell to through plasmodesmata, while TGB2 TGB3 are membrane-spanning proteins associated with endoplasmic reticulum-derived granular vesicles. Here, we show organizes PVX "X-body," induced inclusion structure, by remodeling actin endomembranes (endoplasmic reticulum Golgi). Within X-body, forms...
Summary Plant virus‐based vectors carrying sequences homologous to endogenous genes trigger silencing through a homology‐dependent RNA degradation mechanism. This phenomenon, called virus‐induced gene (VIGS), has potential as powerful reverse‐genetics tool in functional genomic programmes transient, loss‐of‐function screens. Here, we describe method enhance the robustness of VIGS phenotype by increasing level dsRNA molecule production, critical step response. Incorporation 40–60 base direct...
Abstract Virus induced gene silencing (VIGS) is increasingly used to generate transient loss-of-function assays and has potential as a powerful reverse-genetics tool in functional genomic programs more rapid alternative stable transformation. A previously described potato virus X (PVX) VIGS vector been shown trigger the permissive host Nicotiana benthamiana. This paper demonstrates that PVX-based also effective triggering response both diploid cultivated tetraploid Solanum species. We show...
Abstract Due to their capability of eliciting a form posttranscriptional gene silencing (termed virus-induced or VIGS), plant viruses are increasingly used as reverse-genetics tools for functional characterization genes. RNA have been shown trigger in variety host plants, including members Solanacae and Arabidopsis (Arabidopsis thaliana). Several factors affect the response, range viral tropism within plant. The work presented here demonstrates that modified tobacco rattle virus (TRV) vector...
We describe a method for localizing plant viral RNAs in vivo using Pumilio, an RNA-binding protein, coupled to bimolecular fluorescence complementation (BiFC). Two Pumilio homology domain (PUMHD) polypeptides, fused either the N- or C-terminal halves of split mCitrine, were engineered recognize two closely adjacent eight-nucleotide sequences genomic RNA tobacco mosaic virus (TMV). Binding PUMHDs their target sites brought mCitrine into close proximity, allowing BiFC occur and revealing...
• Multidrug and toxic compound extrusion (MATE) proteins comprise the most recently identified family of multidrug transporters. In plants, numbers MATE has undergone a remarkable expansion, underscoring importance these transporters within this kingdom. Here, we describe identification characterization Activated Disease Susceptibility 1 (ADS1) which encodes putative transport protein. An activation tagging screen uncovered ads1-Dominant (ads1-D) mutant, was subsequently characterized by...
BAX INHIBITOR-1 (BI-1) is one of the few proteins known to have cross-kingdom conserved functions in negative control programmed cell death. Additionally, barley BI-1 (HvBI-1) suppresses defense responses and basal resistance powdery mildew fungus Blumeria graminis f. sp. hordei enhances death-provoking fungi when overexpressed barley. Downregulation HvBI-1 by transient-induced gene silencing or virus-induced limited susceptibility B. hordei, suggesting that a factor toward mildew. Transient...
Abstract The European Commission requested the EFSA Panel on Plant Health to evaluate probability of entry pests (likelihood pest freedom at entry), including both, regulated and non-regulated pests, associated with unrooted cuttings genera Petunia Calibrachoa produced under physical isolation in Guatemala. relevance any for this opinion was based evidence following defined criteria, methodology used high-risk plants adapted specificity assessment. Nineteen EU (Bemisia tabaci, pepper golden...
Summary A novel myb oncogene homologue ( AtMYB30 ) has been isolated by differential screening of a cDNA library prepared from Xanthomonas campestris pv. X. )‐inoculated Arabidopsis thaliana cells cultured in the presence cycloheximide. is single‐copy gene, and encoded protein contains MYB domain highly homologous to other plant animal proteins. Analyses transcript levels A. plants, or infected with either virulent avirulent strains pathogens Pseudomonas syringae tomato, showed that maximal...
New molecular markers of the hypersensitive response (HR) Arabidopsis thaliana to bacterial pathogen Xanthomonas campestris pv. ( X.c.c ..) have been identified by differential screening a cDNA library constructed from suspension cells inoculated an HR‐inducing strain in presence cycloheximide. Seven families genes (called Athsr ) isolated, show similarities voltage‐dependent anion channels (VDAC) and alternative oxidases, or are novel proteins. shown be specifically preferentially expressed...
Almost nothing is known of the earliest stages plant virus infections. To address this, we microinjected Cy3 (UTP)-labelled tobacco mosaic (TMV) into living trichome cells. The Cy3-virions were infectious, and viral genome trafficked from cell to cell. However, neither fluorescent vRNA pool nor co-injected green protein (GFP) left injected trichome, indicating that synthesis (unlabelled) progeny (v)RNA required initiate cell-to-cell movement, movement not accompanied by passive...
Replication of Barley stripe mosaic virus (BSMV), genus Hordeivirus, is thought to be associated with vesicles in proplastids and chloroplasts, but the molecular details process identity proteins involved establishing replication complexes are unknown. In addition, BSMV encodes a triple-gene block movement (TGBs) that putatively share functional roles their counterparts other hordei-, pomo- pecluviruses, detailed information on intracellular locations individual TGBs lacking. Here,...
ABSTRACT Storage of potato tubers at low temperatures leads to the accumulation glucose and fructose in a process called ‘cold sweetening’. The aim this work was investigate role sucrose‐phosphatase (SPP) tuber carbohydrate metabolism temperature (4 °C). To end, RNA interference (RNAi) used reduce SPP expression transgenic tubers. Analysis specific small interfering RNAs (siRNAs), protein enzyme activity indicated that silencing stable during cold treatment. soluble carbohydrates showed...