A5041402452- Plant-Microbe Interactions and Immunity
- Plant Pathogens and Fungal Diseases
- Plant Disease Resistance and Genetics
- Plant Pathogens and Resistance
- Insect Resistance and Genetics
- Legume Nitrogen Fixing Symbiosis
- Plant Pathogenic Bacteria Studies
- Banana Cultivation and Research
- Plant and soil sciences
- Plant tissue culture and regeneration
- Plant Virus Research Studies
- Mycorrhizal Fungi and Plant Interactions
- Pineapple and bromelain studies
- Plant Stress Responses and Tolerance
- Insect and Pesticide Research
- Entomopathogenic Microorganisms in Pest Control
- Potato Plant Research
- Plant Parasitism and Resistance
- Yeasts and Rust Fungi Studies
- Sugarcane Cultivation and Processing
- Microbial Metabolites in Food Biotechnology
- Garlic and Onion Studies
- Studies on Chitinases and Chitosanases
- Sentiment Analysis and Opinion Mining
- Viral Infectious Diseases and Gene Expression in Insects
Qilu University of Technology
2017-2024
Shandong Academy of Sciences
2018-2024
Centro de Ingeniería Genética y Biotecnología
2010-2021
Escuela Politécnica del Ejército
2017-2018
National Center for Genetic Engineering and Biotechnology
2010-2015
Wageningen University & Research
2008
Universidad de Ciego de Ávila
1998
Summary During tomato leaf colonization, the biotrophic fungus Cladosporium fulvum secretes several effector proteins into apoplast. Eight effectors have previously been characterized and show no significant homology to each other or fungal genes. To discover novel C. that might play a role in virulence, we utilized two‐dimensional polyacrylamide gel electrophoresis (2D‐PAGE) visualize secreted during –tomato interactions. Three were identified: CfPhiA, Ecp6 Ecp7. CfPhiA shows found on...
Summary A diverse range of plant proteases are implicated in pathogen perception and subsequent signalling execution disease resistance. We demonstrate, using protease inhibitors virus‐induced gene silencing (VIGS), that the papain cysteine cathepsin B is required for resistance hypersensitive response (HR). VIGS prevented programmed cell death (PCD) compromised induced by two distinct non‐host bacterial pathogens. It also suppressed HR triggered transient co‐expression potato R3a...
The molecular signals and pathways that govern biotic abiotic stress responses in sugarcane are poorly understood. Here we describe SodERF3, a (Saccharum officinarum L. cv Ja60-5) cDNA encodes 201-amino acid DNA-binding protein acts as transcriptional regulator of the ethylene responsive factor (ERF) superfamily. Like other ERF transcription factors, SodERF3 binds to GCC box, its deduced amino sequence contains an N-terminal putative nuclear localization signal (NLS). In addition, C-terminal...
Plant defensins are small cysteine-rich peptides that inhibit the growth of a broad range microbes. In this article, we describe NmDef02, novel cDNA encoding putative defensin isolated from Nicotiana megalosiphon upon inoculation with tobacco blue mould pathogen Peronospora hyoscyami f.sp. tabacina. NmDef02 was heterologously expressed in yeast Pichia pastoris, and purified recombinant protein found to display antimicrobial activity vitro against important plant pathogens. Constitutive...
Plants are continuously challenged by pathogens, affecting most staple crops compromising food security. They have evolved different mechanisms to counterattack pathogen infection, including the accumulation of pathogenesis-related (PR) proteins. These proteins been implicated in active defense, and their overexpression has led enhanced resistance nuclear transgenic plants, although many cases constitutive expression resulted lesion-mimic phenotypes. We decided evaluate plastid...
Huanglongbing (HLB) constitutes the most destructive disease of citrus worldwide, yet no established efficient management measures exist for it. Brassinosteroids, a family plant steroidal compounds, are essential growth, development and stress tolerance. As possible control strategy HLB, epibrassinolide was applied to as foliar spray plants infected with causal agent 'Candidatus Liberibacter asiaticus'. The bacterial titers were reduced after treatment under both greenhouse field conditions...
The identification and use of endophytic bacteria capable triggering plant growth is an important aim in sustainable agriculture. In nature, plants live alliance with multiple growth-promoting microorganisms. the current study, we isolated identified a new bacterium from wild species Glyceria chinensis (Keng). was designated as Bacillus altitudinis strain using 16S rDNA sequencing. B. had notable influence on growth. results our assays revealed that raised different species. Remarkably,...
Mosquitoes of many species are key disease vectors, killing millions people each year. Bacillus thuringiensis-based insecticide formulations largely recognized as among the most effective, ecologically safe, and long-lasting methods managing insect pests. New B. thuringiensis strains with high mosquito control effectiveness were isolated, identified, genetically defined, physiologically characterized. Eight identified shown to carry endotoxin-producing genes. Using a scanning electron...
Summary SfT6 has been identified in a subtracted cDNA library of Spodoptera frugiperda larval midgut transcripts as serine‐protease gene downregulated within 24 h intoxication with Bacillus thuringiensis Cry1Ca1 protein. In the present study, specific role during was investigated by RT‐PCR and vivo RNA interference. Quantitative real‐time analysis showed mRNA levels tissue were significantly reduced after injecting or feeding 4th‐instar larvae long‐size dsRNA. Gut juice‐mediated vitro...
Background Allium fistulosum L. has good nutritional value and is cultivated worldwide as an efficacious traditional medicinal plant. Its biological activities are attributable to its phytochemicals. Nitrogen essential nutrient for plant growth development; however, the effect of nitrogen levels on level active components in this species not well understood. Methods In study, using urea fertilizer, we investigated effects different (N0, N1, N2 at 0, 130, 260 kg/ha, respectively)...
Stress caused by pathogens strongly damages plants. Developing products to control plant disease is an important challenge in sustainable agriculture. In this study, a heat-killed endophytic bacterium (HKEB), Bacillus aryabhattai, used induce defense against fungal and bacterial pathogens, the main pathways HKEB activate are revealed. The induced high protection different through salicylic jasmonic acid pathways. We report presence of gentisic for first time. These results show that HKEBs...
In nature, plants interact with a wide range of microorganisms, and most these microorganisms could induce growth through the activation important molecular pathways. The current study evaluated whether endophytic bacterium Bacillus aryabhattai encourages plant transcriptional changes that might be implicated in this effect. promotes Arabidopsis tobacco plants. treated were also identified, results showed various genes, such as cinnamyl alcohol dehydrogenase, apyrase, thioredoxin H8,...
We have tested whether a gene encoding polygalacturonase-inhibiting protein (PGIP) protects tobacco against fungal pathogen (Rhizoctonia solani) and two oomycetes (Phytophthora parasitica var. nicotianae Peronospora hyoscyami f. sp. tabacina). The trials were performed in greenhouse conditions for R. solani P. the field hyoscyami. Our results show that expression of PGIP is powerful way engineering broad-spectrum disease resistance.
Peritrophins are associated with structural and functional integrity of peritrophic membranes (PM), structures composed chitin proteins. PM lines the insect midgut has roles in digestion protection from toxins. We report full-length cDNA cloning, molecular characterization analysis SfPER, a novel peritrophin A protein, Spodoptera frugiperda. The predicted amino acid sequence indicated SfPER's domain structure as CMCMC-type, consisting signal peptide three chitin-binding (C) domains two...
Fungal diseases lead to significant losses in soybean yields and a decline seed quality; such is the case of Asian rust anthracnose caused by Phakopsora pachyrhizi Colletotrichum truncatum, respectively. Currently, development transgenic plants carrying antifungal defensins offers an alternative for plant protection against pathogens. This paper shows production expressing NmDef02 defensin gene using biolistic delivery system, attempt improve resistance reduce need chemicals. Transgenic...
Epothilones constitute a new class of microtubule-stabilizing anti-cancer agents with promising preclinical and clinical activity. However, its systemic application still causes some toxic side effects. To reduce these undesired effects, advanced drug delivery systems based on cell targeting carriers are needed currently. In this study, the high quality bacterial ghosts probiotic Escherichia coli Nissle 1917 (EcN) were prepared in large scale retained fully intact surface structures for...