A5057545215- Plant tissue culture and regeneration
- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Plant nutrient uptake and metabolism
- Plant-Microbe Interactions and Immunity
- Plant Reproductive Biology
- Plant Gene Expression Analysis
- Seed Germination and Physiology
- Cancer Research and Treatments
- Fungal and yeast genetics research
- Postharvest Quality and Shelf Life Management
- Endometrial and Cervical Cancer Treatments
- Ovarian cancer diagnosis and treatment
- Enzyme Production and Characterization
- Plant Physiology and Cultivation Studies
- Phytase and its Applications
- Transgenic Plants and Applications
- Aquaculture disease management and microbiota
- Photosynthetic Processes and Mechanisms
- Legume Nitrogen Fixing Symbiosis
- Chromosomal and Genetic Variations
- Vibrio bacteria research studies
- Wheat and Barley Genetics and Pathology
- Probiotics and Fermented Foods
- 14-3-3 protein interactions
Heriot-Watt University
2007-2024
Methodist Hospital
1998-2021
Arizona Oncology
2021
Augusta University
2021
Broward Health
2021
Cancer Research Center
2021
Billings Clinic
2021
Nebraska Methodist College
2020
Houston Methodist
2020
Methodist Hospital
2020
The Arabidopsis ABI1 locus is essential for a wide spectrum of abscisic acid (ABA) responses throughout plant development. Here, was shown to regulate stomatal aperture in leaves and mitotic activity root meristems. gene cloned predicted encode signaling protein. Although its carboxyl-terminal domain related serine-threonine phosphatase 2C, the protein has unique amino-terminal extension containing an EF hand calcium-binding site. These results suggest that Ca 2+ -modulated functions...
Abstract The Arabidopsis (Arabidopsis thaliana) MKK1 and MKK2 mitogen-activated protein kinase kinases have been implicated in biotic abiotic stress responses as part of a signaling cascade including MEKK1 MPK4. Here, the double loss-of-function mutant (mkk1/2) is shown to marked phenotypes development disease resistance similar those single mekk1 mpk4 mutants. Because mkk1 or mkk2 mutants appear wild type, basal levels MPK4 activity are not impaired them, functionally redundant unchallenged...
Nearly 7000 Arabidopsis thaliana ‐expressed sequence tags (ESTs) from 10 cDNA libraries have been sequenced, of which almost 5000 non‐redundant submitted to the EMBL data bank. The quality used is analysed. Similarity searches in international protein banks allowed detection significant similarities a wide range proteins many organisms. Alignment with ESTs rice systematic sequencing project has amino acid motifs are conserved between two organisms, thus identifying genes encoding highly...
• MAP kinases have been linked to guard cell signalling. Arabidopsis thaliana Kinase 3 (MPK3) is known be activated by abscisic acid (ABA) and hydrogen peroxide (H2O2), which also control stomatal movements. We therefore studied the possible role of MPK3 in signalling through cell-specific antisense inhibition expression. Such transgenic plants contained reduced levels mRNA cells displayed partial insensitivity ABA opening, but responded normally this hormone closure. However, ABA-induced...
Abstract The modulation of mitogen-activated protein kinase (MAPK) activity regulates many intracellular signaling processes. In animal and yeast cells, MAP kinases are activated via phosphorylation by the dual-specificity MEK (MAP kinase). Several plant homologs MAPK have been identified, but biochemical events underlying activation MAPKs remain unknown. We describe in vitro an Arabidopsis homolog kinase, ATMPK4. ATMPK4 was phosphorylated homolog, AtMEK1. This occurred principally on...
The early‐methionine‐labelled (Em) polypeptide is the single most abundant cytosolic protein of dry wheat embryos. It encoded by messenger RNA which accumulates during later (maturation) stages embryogenesis. accumulation Em mRNA can be induced in isolated developing embryos, culture, application plant growth regulator, abscisic acid, prevents precocious germination. Precocious germination also inhibited culture embryos under conditions osmotic stress when induced. This induction occurs...
Summary The mitogen‐activated protein kinase (MAP kinase) signal transduction cascades are routes through which eukaryotic cells deliver extracellular messages to the cytosol and nucleus. These signalling pathways direct cell division, cellular differentiation, metabolism, both biotic abiotic stress responses. In plants, MAP kinases upstream components of represented by multigene families, organized into different stimulated interact in complex ways. Experimental strategies for analysis...
A possible MAP kinase (MAPK) cascade of Arabidopsis thaliana was identified on the basis both yeast 2-hybrid analysis and complementation mutants. Specific protein-protein interactions between ATMPK4 (a MAPK) MEK1 MAPKK) ATMEKK1 MAPKKK) were detected by using system. growth defect mpk1delta mutant reversed coexpression MEK1. Coexpression N-terminal deletion form increased ability to suppress a pbs2delta mutant. These results suggest that ATMPK4, MEK1, may interact with each other constitute...
14-3-3 proteins are important eukaryotic regulatory proteins. Barley (Hordeum vulgare L.) 14-3-3A was over-expressed, immobilised and used to affinity purify binding from developing barley grains. Binding shown be phosphorylation-dependent. These were fractionated by PAGE identified MALDI-TOF MS. In total, 54 identified, 49 of these interactions novel plants. fell into a number functional categories. The largest category for carbohydrate metabolism, including plastidic enzymes starch...
ABSTRACT A characteristic feature of bacteriophage genomes is that they are architecturally mosaic, with each individual genome representing a unique assemblage exchangeable modules. Plausible mechanisms for generating mosaicism include homologous recombination at shared boundary sequences module junctions, illegitimate in non-sequence-directed process, and site-specific recombination. Analysis the novel mycobacteriophage Giles not only extends our current perspective on genetic diversity,...
The MYB-related gene ASYMMETRIC LEAVES 1 (AS1) and its orthologs have an evolutionarily conserved role in specification of leaf cell identity. AS1 is expressed founder cells, where it functions as a heterodimer with the structurally unrelated AS2 proteins to repress activity KNOTTED 1-like homeobox (KNOX) genes. therefore confines KNOX shoot apical meristem, promotes stem function through regulation phytohormone activities. Here, we show that loss-of-function mutations unexpectedly convey...
Drought is a major threat to world agriculture. In order identify proteins associated with plant drought tolerance, barley varieties bred in the UK ( G olden P romise) and I raq B asrah) were compared. The variety asrah showed physiological adaptations when compared romise, for example relative water content of roots shoots after 1 week was much higher than romise. DIGE analysis carried out on from leaves under control conditions. Twenty‐four leaf 45 root identified by MALDI ‐ TOF MS ....