A5047756593- Lysosomal Storage Disorders Research
- Ovarian function and disorders
- Reproductive Biology and Fertility
- Ubiquitin and proteasome pathways
- RNA Research and Splicing
- Curcumin's Biomedical Applications
- Nanoparticles: synthesis and applications
- Microplastics and Plastic Pollution
- Metabolism and Genetic Disorders
- Child Nutrition and Feeding Issues
- Sperm and Testicular Function
- Moyamoya disease diagnosis and treatment
- Autoimmune and Inflammatory Disorders Research
- Retinal Diseases and Treatments
- Biochemical effects in animals
- TGF-β signaling in diseases
- Sulfur Compounds in Biology
- Aldose Reductase and Taurine
- Infectious Encephalopathies and Encephalitis
- Sleep and Wakefulness Research
- Paraquat toxicity studies and treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Long-Term Effects of COVID-19
- Glaucoma and retinal disorders
- Advanced Glycation End Products research
Aster
2023
Clinica Mediterranea
2023
Sapienza University of Rome
2015-2022
European Brain Research Institute
2020-2022
IRCCS Istituto Auxologico Italiano
2021
Spike protein (S protein) is the virus "key" to infect cells and able strongly bind human angiotensin-converting enzyme2 (ACE2), as has been reported. In fact, structure function known be highly important for cell infection well entering brain. Growing evidence indicates that different types of coronaviruses not only affect respiratory system, but they might also invade central nervous system (CNS). However, very little so far reported on presence COVID-19 in brain, potential exploitation,...
Abstract Mice exposed to continuous light undergo functional and histological changes that mimic those of human Polycystic Ovary Syndrome (PCOS). We herein induced the syndrome by exposing 30‐day‐old females 10 weeks permanent light. Ovarian morphology histology, as well reproductive parameters (time observed pregnancy/delivery) were investigated. Ovaries PCOS‐modeled mice showed lack tertiary follicles corpora lutea, altered ovarian architecture, increased thickness theca layer. When...
The Niemann-Pick type C1 (NPC1) disease is a neurodegenerative lysosomal storage disorder due to mutations in the NPC1 gene, encoding transmembrane protein related Sonic hedgehog (Shh) receptor, Patched, and involved intracellular trafficking of cholesterol. We have recently found that proliferation cerebellar granule neuron precursors significantly reduced Npc1−/− mice downregulation Shh expression. This finding prompted us analyze formation primary cilium, non-motile organelle specialized...
The nuclear RNA-binding protein TDP-43 forms abnormal cytoplasmic aggregates in the brains of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD) patients several molecular mechanisms promoting mislocalization aggregation have been proposed, including defects nucleocytoplasmic transport, stress granules (SG) disassembly post-translational modifications (PTM). SUMOylation is a PTM which regulates variety cellular processes and, similarly to ubiquitination, targets lysine...
The ubiquitous spread of Polystyrene nanoplastics (PS-NPs) has rendered chronic human exposure an unavoidable phenomenon. biodistribution such particles leads to bioaccumulation in target organs including the testis, site sperm maturation. purpose this research been estimate impact PS-NPs (50 and 100 nm) on metabolism mature spermatozoa. analysis semen parameters revealed a higher toxicity smaller sized PS-NPs, which have negatively affected major organelles, leading increased acrosomal...
Abstract Niemann-Pick Type C1 disorder (NPC) is a rare lysosomal storage disease characterized by the accumulation of cholesterol in lysosomes. NPC has no FDA approved treatments yet, however 2-hydroxypropyl-β-cyclodextrin (HPβCD) shown efficacy for treating both mouse and feline models currently being investigated late stage clinical trials. Despite promising results, therapeutic use HPβCD limited need high doses, ototoxicity intrathecal administration. These limitations can be attributed...
Niemann-Pick type C1 (NPC1) disease is a lysosomal storage disorder caused by defective intracellular trafficking of exogenous cholesterol. Purkinje cell (PC) degeneration the main sign cerebellar dysfunction in both NPC1 patients and animal models. It has been recently shown that significant decrease Sonic hedgehog (Shh) expression reduces proliferative potential granule neuron precursors developing cerebellum Npc1 −/− mice. Pursuing hypothesis this developmental defect translates into...
Administration of 1000–1500 mg/day D-Chiro-Inositol (DCIns) or a combination Myo-Inositol (MyoIns) and DCIns in their plasma molar ratio (40:1) for three more months are among recommended treatments metabolic syndrome and/or Polycystic Ovary Syndrome (PCOS). We previously confirmed the efficacy this formulation (8.2 MyoIns 0.2 10 days) mouse PCOS model, but also observed negative effects on ovarian histology function formulations containing 0.4–1.6 DCIns. therefore analyzed higher doses...
<b><i>Objectives:</i></b> Luteinizing hormone (LH) plays a key role in normal follicular development and oocyte maturation controlled ovarian stimulation. LH stimulates the proliferation differentiation of theca cells for secretion androgens, synergistically increasing estrogen production. This study aimed to investigate effects low concentrations on retrieval, fertilization, embryo patients undergoing vitro fertilization/intracytoplasmic sperm injection....
Abstract Retinal ganglion cell (RGC) loss is a pathologic feature common to several retinopathies associated optic nerve damage, leading visual and blindness. Although scientific efforts have been spent understand the molecular cellular changes occurring in retinal degeneration, an effective therapy counteract damage still not available. Here we show that eyeballs, enucleated with concomitant cut (ONC), when kept PBS for 24 h showed degeneration. Examining retinas nerves at different time...
Abstract Proteins belonging to the TGFβ‐stimulated clone 22 domain (TSC22D) family display a repertoire of activities, regulating cell proliferation and differentiation. The tumor suppressor activity first identified member family, TSC22D1 (formerly named TSC‐22), has been extensively studied, but afterward longer isoform encoded by same gene turned out play an opposite role. We have previously characterized role TSC22D4 in differentiation using granule neurons (GNs) isolated from mouse...
The loss of NPC1 or NPC2 function results in cholesterol and sphingolipid dyshomeostasis that impairs developmental trajectories, predisposing the postnatal brain to appearance pathological signs, including progressive stereotyped Purkinje cell microgliosis. Despite increasing evidence reporting activation pro-inflammatory microglia as a cardinal event disease progression at symptomatic stages both patients preclinical models, how cells respond altered neurodevelopmental dynamics remains not...
Retinal degeneration is the major and principal cause behind many incurable blindness diseases. Several studies indicated neuroprotective effect of Curcuma longa in eye pathologies, specifically retinopathy. However, molecular mechanism its has not been completely elucidated. Using an ex vivo model retinal obtained from optic nerve cut (ONC), we demonstrated that extract (Cur) exerted a effect. Importantly, Cur was able to modulate apoptosis MAPK signaling pathway activation prevent ganglion...
Nowadays, developing countries have seen a reduction in male reproductive parameters, and it has been linked to the exposure of endocrine disrupting chemicals (EDCs), which are able mimic or disrupt steroid hormone actions. Also, nanoparticles shown effects on system, particular use TiO
Abstract Luteinizing hormone plays a key role in normal follicular development and oocyte maturation controlled ovarian stimulation. stimulates the proliferation differentiation of theca cells for secretion androgens, synergistically increasing estrogen production. This study aimed to investigate effects low luteinizing concentrations on retrieval, fertilization embryo patients undergoing vitro fertilization/intracytoplasmic sperm injection. We prospectively analyzed injection, subdividing...
Abstract The nuclear RNA-binding protein TDP-43 forms abnormal cytoplasmic aggregates in the brains of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD) patients several molecular mechanisms promoting mislocalization aggregation have been proposed, including defects nucleocytoplasmic transport, stress granules (SG) disassembly post-translational modifications (PTM). SUMOylation is a PTM which regulates variety cellular processes and, similarly to ubiquitination, targets...