A5071624853- Plant Virus Research Studies
- Plant and Fungal Interactions Research
- Phytoplasmas and Hemiptera pathogens
- Plant Pathogenic Bacteria Studies
- Insect-Plant Interactions and Control
- Plant Disease Resistance and Genetics
- Plant Disease Management Techniques
- Cocoa and Sweet Potato Agronomy
- Plant-Microbe Interactions and Immunity
- Sugarcane Cultivation and Processing
- Animal Virus Infections Studies
- Horticultural and Viticultural Research
- Viral Infections and Immunology Research
- Rice Cultivation and Yield Improvement
- Plant Pathogens and Fungal Diseases
- Plant tissue culture and regeneration
- CRISPR and Genetic Engineering
- Plant Parasitism and Resistance
- Insect symbiosis and bacterial influences
- Polyomavirus and related diseases
- Nematode management and characterization studies
- Transgenic Plants and Applications
- Photosynthetic Processes and Mechanisms
- Legume Nitrogen Fixing Symbiosis
- Plant pathogens and resistance mechanisms
Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures
2018-2025
Leibniz Association
2018-2024
Pennsylvania State University
2014-2022
Institute for Sustainable Plant Protection
2014-2018
Centro di Ricerca per la Patologia Vegetale
2007-2014
Istituto Nazionale di Fisica Nucleare, Sezione di Torino
2014
Consejo Nacional de Investigaciones Científicas y Técnicas
2012
National Academies of Sciences, Engineering, and Medicine
2011
National Research Council
2011
All known pepper cultivars resistant to Tomato spotted wilt virus (TSWV) possess a single dominant resistance gene, Tsw. Recently, naturally occurring resistance-breaking (RB) TSWV strains have been identified, causing major concerns. We used collection of such identify the specific genetic determinant that allows overcome Tsw gene in Capsicum spp. A reverse approach is still not feasible for TSWV; therefore, we analyzed reassortants between wild-type (WT) and RB strains. Our results...
Tomato spotted wilt virus (TSWV) is the type member of tospoviruses (genus Tospovirus), plant-infecting viruses that cause severe damage to ornamental and vegetable crops. Tospoviruses are transmitted by thrips in circulative propagative mode. We generated a collection NSs-defective TSWV isolates showed coding for truncated NSs protein could not be Frankliniella occidentalis. Quantitative reverse transcription (RT)-PCR immunostaining individual insects detected mutant second-instar larvae...
Abstract Background Translational and post-translational protein modifications play a key role in the response of plants to pathogen infection. Among latter, phosphorylation is critical modulating structure, localization interaction with other partners. In this work, we used multiplex staining approach 2D gels study quantitative changes proteome phosphoproteome Flavescence dorée-affected recovered ‘Barbera’ grapevines, compared healthy plants. Results We identified 48 proteins that...
Flavescence dorée is a serious phytoplasma disease affecting grapevine in several European countries. We studied the interaction of with its natural plant host by monitoring effects infection on protein expression profile. Among 576 analyzed spots, 33 proteins were differentially regulated infected grapevines. Grouping into MIPS functional categories showed involved metabolism (21%), energy processes (9%), synthesis (3%), fate (18%), cellular transport and routes (6%), cell defense virulence...
Flavescence dorée phytoplasma (FDp) infections seriously affect production and survival of grapevine. We analysed the changes in flavonoid pathway occurring two red cultivars, highly susceptible Barbera less Nebbiolo, following FDp infection. A combination metabolic transcript analyses was used to quantify compounds expression a set genes involved their biosynthesis. Quantification anthocyanins, flavonols, proanthocyanidins related biosynthetic enzymes performed over vegetative season, at...
Cassava witches' broom disease (CWBD) has emerged as a significant threat to cassava production in the Oiapoque region of Amap&aacute, Brazil. Diseased plants exhibit stunted growth, vascular necrosis, abnormal shoot proliferation, and distinctive broom-like appearance. This study aimed characterize identify its causal agent(s). The assessment by high-throughput sequencing total nucleic acids (DNA RNA) from affected tissues revealed Rhizoctonia theobromae (syn. Ceratobasidium )...
RNA viruses of the genera Ambivirus, Mitovirus, Sclerotimonavirus, and Partitivirus were found in a single isolate Fusarium graminearum. The genomes mitovirus, sclerotimonavirus, partitivirus assigned to previously described viruses, whereas ambivirus genome putatively represents new species, named graminearum 1 (FgAV1). To investigate effect mycoviruses on fungal phenotype, spontaneous loss during meiosis transmission into strain via anastomosis used obtain isogenic F. strains both with...
Tomato spotted wilt virus (TSWV) is endemic in the Almería region of Spain, where local agro-climatic conditions allow Frankliniella occidentalis, main vector TSWV, continuous generations throughout year. So far, only efficient method to control for this pepper crops has relied on Tsw resistance gene. During autumn 2003, some varieties previously shown be resistant TSWV displayed symptoms commonly associated with infection. A serological test carried out field lateral flow devices prepared...
Flavescence dorée (FD) is considered one of the most severe phytoplasma diseases affecting grapevine. The spontaneous, complete, and stable remission symptoms FD (recovery) a phenomenon that may occur in infected grapevines. molecular bases this are still unclear, although some works suggest recovery could be linked to accumulation hydrogen peroxide (H 2 O ). Several genes coding for enzymes involved H metabolism, ascorbate-glutathione cycle, defense responses, biosynthesis hormones were...
Multiple detection of the phytoplasmas associated with Flavescence dorée (FD) and Bois noir (BN) diseases viruses Grapevine leafroll virus ‐1 ‐3 ( Ampelovirus ) A Vitivirus is described, using same crude extract as template. Sap was prepared by semi‐automatic maceration requiring minimal time effort, consisting a tissue grinding step in carbonate buffer boiling glycine buffer; two microlitres were used template each pathogen‐specific assay. RealTime reverse transcription (RT)‐PCR for FD...
We report here efforts to benchmark performance of two widespread approaches for virome analysis, which target either virion-associated nucleic acids (VANA) or highly purified double-stranded RNAs (dsRNAs). This was achieved using synthetic communities varying complexity levels, up a complex community 72 viral agents (115 molecules) comprising isolates from 21 families and 61 genera plant viruses. The results obtained confirm that the dsRNA-based approach provides more complete...
RNA-viruses of the genera Ambivirus, Mitovirus, Sclerotimonavirus, and Partitivirus were found in a single isolate Fusarium graminearum. The genomes mitovirus, sclerotimonavirus, partitivirus assigned to previously described viruses, whereas ambivirus genome putatively represents new species, named graminearum 1 (FgAV1). To investigate effect mycoviruses on fungal phenotype, spontaneous loss during meiosis transmission into strain by anastomosis used obtain isogenic F. strains with without...
The rootstocks <i>Vitis rupestris</i> cv. St. George, Kober 5BB, LN33, and Freedom were graft-inoculated with viruses associated the rugose wood complex (RW) to study their growth response symptom expression. virus sources used in this isolates LR127, CB111, CB105, PA94-142 for <i>Grapevine A</i> (GVA), CB120 B</i> (GVB), LV92-07 a field selection of Chardonnay D</i> (GVD), Cabernet Sauvignon rupestris stem pitting-associated virus</i> (GRSPaV). Each rootstock plant was one, two, or three...
The widespread use of High-Throughput Sequencing (HTS) for detection plant viruses and sequencing virus genomes has led to the generation large amounts data bioinformatics challenges process them. Many pipelines are available, making choice a suitable one difficult. A robust benchmarking is needed unbiased comparison pipelines, but there currently lack reference datasets that could be used this purpose. We present 7 semi-artificial composed real RNA-seq from virus-infected plants spiked with...
Since the first report in 2009, at least ten additional viruses have been identified and assigned to proposed virus family Alternaviridae. Here we two new mycoviruses tentatively this family, both as members of fungal Nectriaceae, which were isolated from surface-disinfected apple roots (Malus x domestica, Borkh.) affected by replant disease (ARD). ARD is a highly complex, worldwide-occurring resulting plant reactions disturbed (micro)-biome leads high economic losses every year. The...