A5084871588- Trace Elements in Health
- Molecular Junctions and Nanostructures
- Smoking Behavior and Cessation
- Iron Metabolism and Disorders
- Epigenetics and DNA Methylation
- Lipid Membrane Structure and Behavior
- RNA modifications and cancer
- Drug Transport and Resistance Mechanisms
- Electrochemical sensors and biosensors
- Gut microbiota and health
- thermodynamics and calorimetric analyses
- Protein Interaction Studies and Fluorescence Analysis
- Biochemical Analysis and Sensing Techniques
- Calpain Protease Function and Regulation
- Folate and B Vitamins Research
- Protein Structure and Dynamics
- Heavy Metal Exposure and Toxicity
University of California, Davis
2022-2023
University of Southern California
2019-2022
University of California, Santa Barbara
2018
We have investigated the regulation of genes and associated molecular pathways, genome-wide, in oral cells electronic cigarette (e-cigs) users smokers as compared to non-smokers. Interrogation transcriptome by RNA-sequencing (RNA-seq) analysis showed significant number aberrantly expressed transcripts both e-cig (vapers) relative non-smokers; however, had ~50% more differentially than vapers (1726 versus 1152). Whereas deregulated were predominately from protein-coding (79% 53% vapers),...
The outbreak of vaping-related severe lung injuries and deaths the epidemic teen vaping in U.S. underscore urgent need for determining biological consequences electronic cigarette (e-cig) use. We have investigated association between epigenetic changes by quantifying DNA methylation levels Long Interspersed Nucleotide Element 1 (LINE-1) global hydroxymethylation (5-hmC) measuring expression level enzymes catalysing respective processes peripheral blood exclusive vapers, smokers, controls,...
Iron is an essential biometal, critical in processes that include oxygen transport, mitochondrial respiration, and cell signaling. dyshomeostasis linked with hyperglycemia associated metabolic disorders, but the underlying mechanisms are poorly understood. To investigate these mechanisms, we conducted a short-term, four-week, vivo study on mice given water supplemented glucose. The short time frame was sufficient to cause shifts liver towards triglyceride synthesis. We sought comprehensively...
Whereas proteins generally remain stable upon interaction with biological surfaces, they frequently unfold on and adhere to artificial surfaces. Understanding the physicochemical origins of this discrepancy would facilitate development protein-based sensors other technologies that require surfaces do not compromise protein structure function. To date, however, only a small number such have been reported, physics why these support functional biomolecules while others has established. Thus...
Despite the importance of protein-surface interactions in both biology and biotechnology, our understanding their origins is limited due to a paucity experimental studies thermodynamics behind such interactions. In response, we have characterized extent which interaction with chemically well-defined macroscopic surface alters stability protein L. To do so, site-specifically attached redox-reporter-modified variant hydroxy-terminated monolayer on gold then used electrochemistry monitor its...
Background: The clinical manifestations of Wilson disease (WD) are related to copper accumulation in the liver and brain, but little is known about other tissue involvement regarding metabolic changes WD. In vitro studies suggested that loss intestinal ATP7B affects dysregulation We tested this hypothesis by evaluating gut microbiota lipidome 2 mouse models WD characterizing a new model with targeted deletion Atp7b intestine. Methods: Cecal content 16S sequencing untargeted hepatic plasma...
Abstract Copper is essential in a host of biological processes, and disruption its homeostasis associated with diseases including neurodegeneration metabolic disorders. Extracellular copper shifts speciation between healthy disease states, identifying molecular components involved these perturbations could widen the panel biomarkers for status. While there have been exciting advances approaches studying extracellular proteome mass spectrometry–based methods, typical workflows disrupt...
Abstract Despite the importance of protein–surface interactions in both biology and biotechnology, our understanding their origins is limited due to a paucity experimental studies thermodynamics behind such interactions. In response, we have characterized extent which interaction with chemically well‐defined macroscopic surface alters stability protein L. To do so, site‐specifically attached redox‐reporter‐modified variant hydroxy‐terminated monolayer on gold then used electrochemistry...