A5007081168- Fungal and yeast genetics research
- Fungal Biology and Applications
- Biofuel production and bioconversion
- Enzyme Production and Characterization
- Mycorrhizal Fungi and Plant Interactions
- RNA and protein synthesis mechanisms
- Microbial Metabolic Engineering and Bioproduction
- Plant Pathogens and Fungal Diseases
- Marine Biology and Environmental Chemistry
- bioluminescence and chemiluminescence research
- Plant tissue culture and regeneration
- Fermentation and Sensory Analysis
- Plant-Microbe Interactions and Immunity
- DNA Repair Mechanisms
- Protist diversity and phylogeny
- Microbial Natural Products and Biosynthesis
- RNA Research and Splicing
- Cell Image Analysis Techniques
- Microbial Metabolism and Applications
Utrecht University
2005-2017
Cancer Genomics Centre
2008-2010
Mycelial fungi play a central role in element cycling nature by degrading dead organic material such as wood. Fungal colonization of substrate starts with the invasion exploring hyphae. These hyphae secrete enzymes that convert into small molecules can be taken up fungus to serve nutrients. Using green fluorescent protein (GFP) reporter, we show for first time Aspergillus niger differentiate respect enzyme secretion; some strongly express glucoamylase gene glaA, while others hardly it at...
ABSTRACT The fungus Aspergillus niger forms (sub)millimeter microcolonies within a liquid shaken culture. Here, we show that such are heterogeneous with respect to size and gene expression. Microcolonies of strains expressing green fluorescent protein (GFP) from the promoter glucoamlyase glaA or ferulic acid esterase faeA were sorted on basis diameter fluorescence using Complex Object Parametric Analyzer Sorter (COPAS) technology. Statistical analysis revealed culture consisted two...
Summary Colonization of a substrate by fungi starts with the invasion exploring hyphae. These hyphae secrete enzymes that degrade organic material into small molecules can be taken up fungus to serve as nutrients. We previously showed only part Aspergillus niger highly express glucoamylase gene glaA . This was an unexpected finding since all are exposed same environmental conditions. Using GFP reporter, we here demonstrate acid amylase aamA , α‐glucuronidase aguA and feruloyl esterase faeA...
Phleomycin is mutagenic by introducing double-strand breaks in DNA. The ble gene of Streptoalloteychus hindustanus, which confers resistance to this substance, widely used as a selection marker for transformation. Schizophyllum commune grows on 25 microg phleomycin ml(-1) after introduction cassette based the gene. However, we here report that growth resistant colonies concentration resulted aberrant colony morphologies. Apparently, was despite acquired resistance. Therefore, new system...
Black pigmented conidia of Aspergillus niger give rise to micro-colonies when incubated in liquid shaken medium. These are heterogeneous with respect gene expression and size. We here studied the biophysical properties a control strain strains which fwnA, olvA or brnA is inactivated. form fawn-, olive-, brown-coloured conidia, respectively. The ΔolvA produced larger (3.8 μm) compared other (3.2-3.3 μm). Moreover, were highly hydrophilic, whereas those hydrophobic. zeta potential medium was...
Hyphae of ascomycetes are compartmentalized by septa. The central pore in these septa allows for cytoplasmic streaming. However, many pores closed Woronin bodies Aspergillus, which prevents mixing and thus maintains hyphal heterogeneity. Here, glucose uptake transport was studied Aspergillus niger. Glucose higher the population with high transcriptional activity when compared to low activity. transported from colony center periphery, but not vice versa. This unidirectional flow similar...
In both natural and man-made environments, microorganisms live in mixed populations, while laboratory conditions monocultures are mainly used. Microbial interactions often described as antagonistic, but can also be neutral or cooperative, generally associated with a metabolic change of each partner cause the pattern produced bioactive molecules. A. niger oryzae two filamentous fungi widely used industry to produce various enzymes (e.g. pectinases, amylases) metabolites citric acid). The...
Aspergillus niger forms aerial hyphae and conidiophores after a period of vegetative growth. The within the mycelium A. are divided by septa. central pore in these septa allows for cytoplasmic streaming. Here, we studied inter- intra-compartmental streaming reporter protein GFP niger. Expression gene encoding nuclear targeted from gpdA or glaA promoter resulted strong fluorescence nuclei weak structures. These data run on experiments showed that higher expressed when compared to hyphae,...
The presence of a mannitol cycle in fungi has been subject to discussion for many years. Recent studies have found no evidence the this and its putative role regenerating NADPH. However, all enzymes could be measured cultures Aspergillus niger. In study we analyzed localization two from pathway, dehydrogenase mannitol-1-phosphate dehydrogenase, expression their encoding genes nonsporulating sporulating A. Northern analysis demonstrated that mpdA was expressed both mycelia, while mtdA only...