A5035372249- Fungal Biology and Applications
- Mycorrhizal Fungi and Plant Interactions
- Plant Pathogens and Fungal Diseases
- Fungal and yeast genetics research
- Powdery Mildew Fungal Diseases
- Fermentation and Sensory Analysis
- Biofuel production and bioconversion
- Plant and fungal interactions
- Plant Pathogens and Resistance
- Genomics and Phylogenetic Studies
- Enzyme-mediated dye degradation
- Microbial Metabolism and Applications
- Protist diversity and phylogeny
- Plant and Biological Electrophysiology Studies
- Yeasts and Rust Fungi Studies
- Antifungal resistance and susceptibility
- Horticultural and Viticultural Research
- Polysaccharides and Plant Cell Walls
- Plant Stress Responses and Tolerance
- Plant Disease Resistance and Genetics
- Plant tissue culture and regeneration
- Fungal Infections and Studies
- Invertebrate Taxonomy and Ecology
- Slime Mold and Myxomycetes Research
- Phytochemistry and Bioactivity Studies
Duke University
2024-2025
Duke University Hospital
2024-2025
Duke Medical Center
2024-2025
Wageningen University & Research
2018-2024
Fujian Agriculture and Forestry University
2014-2019
Iwate Biotechnology Research Center
2013-2015
Rural Development Administration
2011
Utrecht University
2007-2009
Cancer Genomics Centre
2008-2009
Background Mating-type loci of mushroom fungi contain master regulatory genes that control recognition between compatible nuclei, maintenance nuclei as heterokaryons, and fruiting body development. Regions near mating-type in often show adapted recombination, facilitating the generation novel mating types reducing production self-compatible types. Compared to other fungi, have complex systems, showing both with redundant function (subloci) subloci many alleles. The genomic organization has...
ABSTRACT White rot fungi can degrade lignin and improve the nutritional value of highly lignified biomass for ruminants. We screened excellent fungi‐biomass combinations by investigating improvement digestibility wheat straw, barley oat rapeseed miscanthus, new reed, spent reed from thatched roofs, cocoa shells after colonisation Ceriporiopsis subvermispora (CS), Lentinula edodes (LE), Pleurotus eryngii (PE) (indicated increased in vitro gas production [IVGP]). First, growth was evaluated...
ABSTRACT Fungal sexual reproduction is controlled by the mating-type ( MAT ) locus. In contrast to a majority of species in phylum Basidiomycota that have tetrapolar systems, opportunistic human pathogen Cryptococcus neoformans employs bipolar system, with two mating types and α) determined single locus unusually large (~120 kb) contains more than 20 genes. While several genes are associated development, others control conserved cellular processes (e.g., cargo transport protein synthesis),...
Abstract The selection of appropriate internal control genes (ICGs) is a crucial step in the normalization real-time quantitative PCR (RT-qPCR) data. Housekeeping are habitually selected for this purpose, despite accumulating evidence on their instability. We screened novel, robust ICGs mushroom forming fungus Volvariella volvacea . Nine commonly used and five newly were evaluated expression stability using RT-qPCR data eight different stages life cycle V. Three algorithms consistently...
Mushroom-forming basidiomycetes colonize large areas in nature. Their hyphae are compartmentalized by perforated septa, which usually covered a septal pore cap (SPC). Here, we describe, for the first time, composition and function of SPCs using model system Schizophyllum commune. The SPC S. commune was shown to consist proteinaceous matrix lipid membrane. demonstrated define ultrastructure two main proteins, Spc14 Spc33. Gene spc14 encodes protein 86 amino acids, lacks known domain, signal...
Phleomycin is mutagenic by introducing double-strand breaks in DNA. The ble gene of Streptoalloteychus hindustanus, which confers resistance to this substance, widely used as a selection marker for transformation. Schizophyllum commune grows on 25 microg phleomycin ml(-1) after introduction cassette based the gene. However, we here report that growth resistant colonies concentration resulted aberrant colony morphologies. Apparently, was despite acquired resistance. Therefore, new system...
The initiation of sexual development in the important edible and medicinal mushroom Flammulina velutipes is controlled by special genes at two different, independent, mating type (MAT) loci: HD PR. We expanded our understanding F. system analyzing MAT loci from a series strains. locus houses homeodomain (Hd genes) on separated locations: sublocus HD-a HD-b. HD-b subloci contained strain-specific Hd1/Hd2 gene pairs, crosses between strains with different indicated role mating. function...
In heterothallic basidiomycete fungi, sexual compatibility is restricted by mating types, typically controlled two loci: PR, encoding pheromone precursors and receptors, HD, types of homeodomain transcription factors. We analysed the single mating-type locus commercial button mushroom variety, Agaricus bisporus var. bisporus, related variety burnettii. identified location using genetic map genome information, corresponding to HD locus, PR having lost its role. found mip1 β-fg genes flanking...
Fungal pretreatment of plant biomass is often assessed by using single genotypes and fungal strains, but can the process be improved harmonizing both, thus selecting specific substrate–fungus combinations? To tackle this question, we treated four Miscanthus sinensis with Ceriporiopsis subvermispora strains thoroughly analyzed substrates residues. The M. differed in cellulose, hemicellulose, lignin contents lignin-wise diverged subunit linkage composition incorporation hydroxycinnamic acids...
Mycelia of higher fungi consist interconnected hyphae that are compartmentalized by septa. These septa contain large pores allow streaming cytoplasm and even organelles. The such mycelia is therefore considered to be continuous.Here, we show laser dissection Schizophyllum commune can closed depending on the environmental conditions. most apical septum growing was open when this basidiomycete grown in minimal medium with glucose as a carbon source. In contrast, second third were more than 50%...
Fungal sexual reproduction is controlled by the mating-type (
The hyphae of filamentous fungi are compartmentalized by septa that have a central pore. fungal and septum-associated structures play an important role in maintaining cellular intrahyphal homeostasis. dolipore the higher Basidiomycota (i.e., Agaricomycotina) associated with septal pore caps. Although ultrastructure caps has been studied extensively, neither biochemical composition nor function these organelles is known. Here, we report identification glycoprotein SPC18 was found cap-enriched...
The edible white rot fungus Lentinula edodes possesses a variety of lignin degrading enzymes such as manganese peroxidases and laccases. Laccases belong to the multicopper oxidases, which have wide range catalytic activities including polyphenol degradation synthesis, degradation, melanin formation. exact number laccases in L. is unknown, are their complete properties biological functions. We analyzed draft genome sequence D703PP-9 identified 13 oxidase-encoding genes; 11 sensu stricto,...
Pleurotus ostreatus, one of the most widely cultivated edible mushrooms, produces high numbers spores causing severe respiratory health problems for people, clogging filters and spoilage produce. A non-sporulating commercial variety (SPOPPO) has been successfully introduced into market in 2006. This was generated by introgression breeding a natural mutation variety. Our cytological studies revealed that meiosis derived sporeless strains blocked metaphase I, apparently resulting loss spore...
Abstract Agaricus bisporus , the most cultivated edible mushroom worldwide, is represented mainly by subspecies var. and burnettii . has a secondarily homothallic life cycle with recombination restricted to chromosome ends, while heterothallic seemingly equally distributed over chromosomes. To better understand relationship between genomic make-up different lifestyles, we have de novo sequenced homokaryon synchronised gene annotations updated versions of published genomes The were assembled...
The edible mushroom Volvariella volvacea is an important crop in Southeast Asia and predominantly harvested the egg stage. One of main factors that negatively affect its yield value rapid transition from to elongation stage, which has a decreased commodity shelf life. To improve our understanding changes during stipe development stage particular, we analyzed gene transcription tissue V. using 3′-tag based digital expression profiling. Stipe turned out be fairly complex with high numbers...
Oyster mushrooms have a high biological efficiency and are easy to cultivate, which is why they produced all over the world. Cap color an important commercial trait for oyster mushrooms. Little known about genetic mechanism of cap in mushrooms, limits molecular breeding improvement color-type cultivars. In this study, 0.8-Mb major quantitative locus (QTL) region controlling mushroom Pleurotus cornucopiae was mapped on chromosome 7 through bulked-segregant analysis sequencing (BSA-seq)...
Abstract Volvariella volvacea is an important crop in Southeast Asia, but erratic fruiting presents a serious challenge for its production and breeding. Efforts to explain inconsistent have been complicated by the multinucleate nature, typical lack of clamp connections, incompletely identified sexual reproductive system. In this study, we addressed life cycle V. using whole genome sequencing, cloning MAT loci, karyotyping spores, assays. Microscopy analysis spores had previously indicated...
Pleurotus ostreatus is a global mushroom crop with nutritional and medicinal benefits. However, the genetic basis of several commercial traits remains unknown. To address this, we analyzed quantitative trait loci (QTLs) for two representative cultivars, "Heuktari" "Miso," apparently distinct alleles. A map 11 linkage groups was constructed, in which 27 QTLs were assigned to 14 traits. The explained phenotypic variations ranged from 7.8% 22.0%. Relatively high LOD values 6.190 5.485 estimated...
White jelly mushroom, Tremella fuciformis, is a popular edible mushroom with interesting medicinal properties (e.g., immunostimulating, antidiabetic). The formation of T. fuciformis basidiomes highly dependent on the presence specific host fungus, both in nature and for industrial production. This has traditionally been indicated as "Xianghui" China, yet which or how many fungal species Xianghui comprises unclear, various authorities claiming different species. At present, Annulohypoxilon...