A5074149535- Ubiquitin and proteasome pathways
- RNA modifications and cancer
- interferon and immune responses
- Viral-associated cancers and disorders
- Microtubule and mitosis dynamics
- Protist diversity and phylogeny
- Renal Transplantation Outcomes and Treatments
- Immune Cell Function and Interaction
- Influenza Virus Research Studies
- Cancer-related Molecular Pathways
- DNA Repair Mechanisms
- Herpesvirus Infections and Treatments
- Marine and coastal ecosystems
- Toxoplasma gondii Research Studies
- Advanced Proteomics Techniques and Applications
- Cancer, Hypoxia, and Metabolism
- RNA regulation and disease
- Microbial Community Ecology and Physiology
- Mitochondrial Function and Pathology
- Cytomegalovirus and herpesvirus research
- Acute Myeloid Leukemia Research
McGill University
2019-2023
University of Toronto
2015-2019
University of New Brunswick
2016
Canada Research Chairs
2016
Concordia University
2014
Herpes simplex virus-1 immediate-early protein ICP0 activates viral genes during early stages of infection, affects cellular levels multiple host proteins and is crucial for effective lytic infection. Being a RING-type E3 ligase prone to auto-ubiquitination, relies on human deubiquitinating enzyme USP7 protection against 26S proteasomal mediated degradation. involved in apoptosis, epigenetics, cell proliferation targeted by several herpesviruses. Several partners, including ICP0, GMPS,...
Abstract The ubiquitin specific protease, USP7, regulates multiple cellular pathways relevant for cancer through its ability to bind and sometimes stabilize target proteins deubiquitylation. To gain a more complete profile of USP7 interactions in cells, we performed affinity purification coupled mass spectrometry identify binding targets gastric carcinoma cells. This confirmed reported associations with USP11, PPM1G phosphatase TRIP12 E3 ligase as well identifying novel two DEAD/DEAH-box RNA...
Abstract The ubiquitin specific protease 7 (USP7 or HAUSP) is known to regulate a variety of cellular processes by binding and deubiquitylating target proteins. To gain more comprehensive understanding its interactions functions, we used affinity purification coupled mass spectrometry profile USP7 interactions. This revealed novel interaction with FBXO38, poorly characterized F-box protein. We showed that stabilizes FBXO38 dependent on catalytic activity protecting from proteasomal...
Myb-like SWIRM and MPN domains 1 (MYSM1) is a chromatin binding protein with deubiquitinase (DUB) catalytic activity. Rare MYSM1 mutations in human patients result an inherited bone marrow failure syndrome, highlighting the biomedical significance of hematopoietic system. We others characterized Mysm1-knockout mice as model this disorder established that regulates function leukocyte development such models through different mechanisms. It is, however, unknown whether DUB activity universally...
We used a genome-wide screen in N-ethyl-N-nitrosourea (ENU)-mutagenized mice to identify genes which recessive loss-of-function mutations protect against pathological neuroinflammation. identified an R367Q mutation the ZBTB7B (ThPOK) protein homozygosity causes protection experimental cerebral malaria (ECM) caused by infection with Plasmodium berghei ANKA. Zbtb7bR367Q homozygous show defect lymphoid compartment expressed as severe reduction number of single-positive CD4 T cells thymus and...