Understanding the mechanisms underlying plant resistance to virus infections is crucial for viral disease management in agriculture. However, defense mechanism of watermelon (Citrullus lanatus) against cucumber green mottle mosaic (CGMMV) infection remains largely unknown. In this study, we performed transcriptomic, metabolomic, and phytohormone analyses a CGMMV susceptible cultivar 'Zhengkang No.2' ('ZK') resistant wild accession PI 220778 (PI) identify key regulatory genes, metabolites,...

Introduction Ethylene (ET) is involved in plant responses to viral infection. However, its molecular mechanisms and regulatory network remain largely unknown. Methods results In the present study, we report that cucumber green mottle mosaic virus (CGMMV) watermelon ( Citrullus lanatus ) triggers ET production by inducing expression of ClACO5 , a key gene biosynthesis pathway through transcriptome data analysis function validation. The knock-down virus-induced silencing overexpressing...

Watermelon silver mottle virus (WSMoV) and melon yellow spot (MYSV) (Tospoviridae, Orthotospovirus) are responsible for mosaic symptoms, posing threats to (Cucumis melo), watermelon (Citrullus lanatus), cucumber leading significant economic losses in China. Early disease detection monitoring of these two viruses necessary management, which a rapid, reliable, adaptable diagnostic method is required. In this study, using droplet digital PCR (ddPCR) method, the conserved region nucleocapsid...

Cucumber green mottle mosaic virus (CGMMV) is a member of the genus Tobamovirus (family Virgaviridae) that causes serious economic losses in cucurbit crops. A possibility for CGMMV control use cross-protection, which stable attenuated isolates are required. In this study, an infectious clone was constructed hn isolate CGMMV. Unexpectedly, carried nonconserved mutation involving single nucleotide change resulting replacement Arg by Cys at residue 284 replicase protein; correlated with delayed...

Abstract RNA silencing, a core part of plants' antiviral defence, requires the ARGONAUTE, DICER‐like, and RNA‐dependent polymerase proteins. However, how these proteins contribute to watermelon's interference (RNAi) pathway response cucumber green mottle mosaic virus (CGMMV) has not been characterized. Here, we identify seven ClAGO , four ClDCL 11 ClRDR genes in watermelon analyse their expression profiles when infected with CGMMV. ClAGO1 ClAGO5 levels were highly induced by CGMMV infection....

It has been reported that squash leaf curl China virus (SLCCNV) infects some Cucurbitaceae crops except for melon (Cucumis melo L.). A new disease of exhibiting severe and dwarfing was observed in Hainan Province China. In this study, the pathogen identified as SLCCNV through biological molecular characterization. The isolate (SLCCNV-HN) possess a bipartite genome, DNA-A (HM566112.1) with highest nucleotide identity (99%) to SLCCNV-Hn (MF062251.1) pumpkin SLCCNV-Hn61 (AM260205.1) isolates...

Squash leaf curl China virus (SLCCNV) is a bipartite Begomovirus. The function of the protein AC5, which encoded by SLCCNV, unknown. Here, we confirmed that 172-amino acids (aa) long AC5 SLCCNV could suppress single-stranded RNA but not double-stranded RNA-induced post-transcriptional gene silencing (PTGS). Furthermore, determined C-terminal domain (96-172 aa) was responsible for suppressor (RSS) activity via deletion mutant analysis. can reverse GFP and inhibit systemic interfering with...

The fungus causing target spot disease, Corynespora cassiicola (Berk. & M. A. Curtis) C. T. Wei, poses an increasing threat to watermelon (Citrullus lanatus), muskmelon (Cucumis melo), and cucumber sativus); the most economically important cucurbit crops grown in China. An understanding of molecular mechanisms underlying pathogenicity is essential for development new strategies control this disease-causing fungus. Agrobacterium tumefaciens-mediated transformation (ATMT) might be useful...

During April 2007, systemic necrotic spots were observed on melon (Cucumis melo) grown in plastic greenhouses Haimen city of Jiangsu Province, China. By mid May disease rates different houses ranged from 19 to 100% with symptoms characteristic Melon spot virus (MNSV). These included many the young leaves followed by coalescence form larger irregular lesions older leaves. Necrotic stripes appeared stems, and infected also became curled wilted. Sap extracts 0·01 M phosphate buffer, pH 7·0,...

During the spring of 2009, a number outbreaks new disease were observed on melon (Cucumis melo) grown in plastic houses Sanya City, Hainan Province, China. Symptoms included numerous yellow, necrotic leaf spots (Fig. 1) and subsequent yellowing 2). By mid-April incidence different ranged from 30% to 100% plants. Suspecting tospovirus infection, DAS-ELISA was performed using specific antisera for Tomato spotted wilt virus (TSWV), Groundnut ringspot (GRSV), Impatiens spot (INSV) Watermelon...

Cucumber green mottle mosaic virus (CGMMV) is one of the most devastating viruses infecting members family Cucurbitaceae. The assembly initiation site CGMMV located in coding region coat protein, which not only involved virion but also a key factor determining long-distance movement virus. To understand effect and adjacent on infectivity, we created GTT deletion mutation GAGGTTG infectious clone CGMMV, termed V97 (deletion at residue 97 protein), followed by construction V94A T104A mutants....

Recombination drives evolution of single-stranded RNA viruses and contributes to virus adaptation new hosts environmental conditions. Intraspecific recombinants are common in potyviruses, the largest family viruses, whereas interspecific rare. Here, we report an recombination event between papaya ringspot potyvirus (PRSV) zucchini tigre mosaic (ZTMV), two potyviruses infecting cucurbit crops sharing similar biological characteristics close phylogenetic relationship. The PRSV-ZTMV were...

Cucumber green mottle mosaic virus (CGMMV), a member of the genus Tobamovirus (family Virgaviridae), is an economically important that has detrimental effects on cucurbit crops worldwide. Understanding interaction between host factors and CGMMV viral proteins will facilitate design new strategies for disease control. In this study, yeast two-hybrid assay revealed helicase (HEL) domain interacts with Citrullus lanatus small heat shock protein (sHSP), we verified observation by performing in...

HomePlant DiseaseVol. 106, No. 5First Report of Watermelon Charcoal Rot (Macrophomina phaseolina) in China PreviousNext DISEASE NOTE OPENOpen Access licenseFirst ChinaHuijie Wu, Chaohan Li, Priyanka Chakraborti, Zhen Guo, Bin Peng, Weihong Gu, Baoshan Kang, and Qinsheng GuHuijie WuZhengzhou Fruit Research Institute, Chinese Academy Agricultural Sciences, Zhengzhou 450009, Henan, China, LiHorticultural Shanghai Sciences/Shanghai Key Lab Protected Horticultural Technology, 201403, Shanghai,...

Potyviral P3 is involved in viral replication, movement, and pathogenicity; however, its biochemical function unknown. In this study, the of zucchini yellow mosaic virus (ZYMV) interacted with ClBBD, a protein high ortholog bifunctional nuclease activity, watermelon. The binding site was shown via yeast two-hybrid screening BiFC assay to be located at N-terminus rather than P3N-PIPO. ClBBD localized predominantly chloroplast plasma membrane. ZYMV also present nucleus cytoplasm as aggregates....

HomePlant DiseaseVol. 103, No. 9First Report of Luffa aphid-borne yellows virus in Cucurbita moschata China PreviousNext DISEASE NOTES OPENOpen Access licenseFirst ChinaB. Peng, B. X. Qin, J. H. Cai, P. Sun, Wu, and Q. S. GuB. Penghttp://orcid.org/0000-0001-8743-153XHenan Provincial Key Laboratory Fruit Cucurbit Biology, Zhengzhou Research Institute, CAAS, Zhengzhou, Henan, 450009, P.R. China, QinGuangxi Biology for Crop Diseases Insect Pests, Plant Protection Guangxi Academy Agricultural...