A5059805465- Plant Virus Research Studies
- Transgenic Plants and Applications
- Plant tissue culture and regeneration
- Plant and Fungal Interactions Research
- Insect-Plant Interactions and Control
- Insect Resistance and Genetics
- Animal Virus Infections Studies
- Plant-Microbe Interactions and Immunity
- Chromosomal and Genetic Variations
- Plant Pathogenic Bacteria Studies
- Plant Disease Resistance and Genetics
- Plant Molecular Biology Research
- Viral gastroenteritis research and epidemiology
- Genomics and Phylogenetic Studies
- Bacteriophages and microbial interactions
- Plant Parasitism and Resistance
- Agricultural pest management studies
- Agricultural Practices and Plant Genetics
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- Monoclonal and Polyclonal Antibodies Research
- Polyomavirus and related diseases
- Virus-based gene therapy research
- Viral Infections and Vectors
- Parvovirus B19 Infection Studies
Addgene
2024
KeyGene (Netherlands)
2009-2022
University of Amsterdam
2018-2021
Netherlands Institute for Neuroscience
2019-2021
Wageningen University & Research
2001-2010
Graduate School Experimental Plant Sciences
1992-2006
RNA silencing or interference (RNAi) is a gene regulation mechanism in eukaryotes that controls cell differentiation and developmental processes via expression of microRNAs. RNAi also serves as an innate antiviral defence response plants, nematodes, insects. This triggered by virus-specific double-stranded molecules (dsRNAs) are produced during infection. To overcome responses, many plant insect viruses encode suppressors (RSSs) enable them to replicate at higher titers. Recently, several...
The species Brassica rapa includes various vegetable crops. Production of these crops is usually impaired by heat stress. Some microRNAs (miRNAs) in Arabidopsis have been considered to mediate gene silencing plant response abiotic However, it remains unknown whether or what miRNAs play a role resistance B. rapa. To identify genomewide conserved and novel that are responsive stress rapa, we defined temperature thresholds non-heading Chinese cabbage (B. ssp. chinensis) constructed small RNA...
Tomato breeding has been tremendously efficient in increasing fruit quality and quantity but did not focus on improving herbivore resistance. The biosynthetic pathway for the production of 7-epizingiberene a wild tomato was introduced into cultivated greenhouse variety with aim to obtain 7-Epizingiberene is specific sesquiterpene toxic repellent properties that produced stored glandular trichomes. We identified synthase (ShZIS) belongs new class synthases, exclusively using...
Summary In nature, plants have to cope with a wide range of stress conditions that often occur simultaneously or in sequence. To investigate how multi‐stress conditions, we analyzed the dynamics whole‐transcriptome profiles Arabidopsis thaliana exposed six sequential double stresses inflicted by combinations of: (i) infection necrotrophic fungus Botrytis cinerea , (ii) herbivory chewing larvae Pieris rapae and (iii) drought stress. Each these induced specific expression over time, which...
Posttranscriptional silencing of a green fluorescent protein (GFP) transgene in Nicotiana benthamiana plants was suppressed when these were infected with Tomato spotted wilt virus (TSWV), plant-infecting member the BUNYAVIRIDAE: Infection TSWV resulted complete reactivation GFP expression, similar to case for Potato Y, but distinct from that Cucumber mosaic virus, two viruses known carry genes encoding suppressor proteins. Agrobacterium-based leaf injections individual identified NS(S) gene...
RNA silencing comprises a set of sequence-specific degradation pathways that occur in wide range eukaryotes, including animals, fungi and plants. A hallmark is the presence small interfering molecules (siRNAs). The siRNAs are generated by cleavage larger double-stranded RNAs (dsRNAs) provide sequence specificity for cognate molecules. In plants, plays key role developmental processes control virus replication. It has been shown many plant viruses encode proteins, denoted suppressors,...
RNA silencing is a natural antiviral defence in plants, which can be exploited transgenic plants for preprogramming virus recognition and ensuring enhanced resistance. By arranging viral transgenes as inverted repeats it thus possible to obtain strong repression of incoming viruses. Due the high sequence specificity silencing, this technology has hitherto been limited targeting single Here shown that efficient simultaneous four different tospoviruses achieved by using small transgene based...
Transcription activator-like effector (TALE) proteins of the plant pathogenic bacterial genus Xanthomonas bind to and transcriptionally activate host susceptibility genes, promoting disease. Plant immune systems have taken advantage this mechanism by evolving TALE binding sites upstream resistance (R) genes. For example, pepper Bs3 rice Xa27 genes are hypersensitive reaction R that activated corresponding TALEs. Both a hallmark expression pattern in which their transcripts detectable only...
ABSTRACT The tospovirus NSs protein was previously shown to suppress the antiviral RNA silencing mechanism in plants. Here biochemical analysis of proteins from different tospoviruses, using purified or containing cell extracts, is described. results showed that all tospoviral analyzed exhibited affinity small double-stranded molecules, i.e., interfering RNAs (siRNAs) and micro-RNA (miRNA)/miRNA* duplexes. Interestingly, tomato spotted wilt virus (TSWV), impatiens necrotic spot (INSV),...
RNA silencing plays a key role in antiviral defense as well developmental processes plants and insects. Negative strand viruses such the plant virus Rice hoja blanca tenuivirus (RHBV) replicate their insect transmission vector. Like most plant-infecting viruses, RHBV encodes an suppressor, NS3 protein, here it is demonstrated that this protein capable of suppressing both cells. Biochemical analyses showed efficiently binds siRNA miRNA molecules. Binding greatly influenced by size small...
Abstract Background Non-coding small RNAs play critical roles in various cellular processes a wide spectrum of eukaryotic organisms. Their responses to abiotic stress have become popular topic economic and scientific importance biological research. Several studies recent years reported number non-coding that map chloroplast genomes. However, it remains uncertain whether are generated from genome how they respond environmental stress, such as high temperature. Chinese cabbage is an important...
We present whole genome profiling (WGP), a novel next-generation sequencing-based physical mapping technology for construction of bacterial artificial chromosome (BAC) contigs complex genomes, using Arabidopsis thaliana as an example. WGP leverages short read sequences derived from restriction fragments two-dimensionally pooled BAC clones to generate sequence tags. These tags are assigned individual clones, followed by assembly based on shared regions containing identical Following in silico...
A tospovirus from onion causing a disease known as “sapeca” by growers in Brazil was characterized. Symptoms on consisted of numerous eyelike spots the leaves and flower stalks resulting abortion. Nicotiana benthamiana N. rustica were only systemic hosts experimentally found. Double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) experiments demonstrated that this virus serologically related to iris yellow spot (IYSV), recently described Netherlands. This virus, onion, based...
In nature, plants are exposed to biotic and abiotic stresses that often occur simultaneously. Therefore, plant responses combinations of most representative how respond stresses. We used RNAseq assess temporal changes in the transcriptome Arabidopsis thaliana herbivory by Pieris rapae caterpillars, either alone or combination with prior exposure drought infection necrotrophic fungus Botrytis cinerea. Pre-exposure stress resulted a significantly different timing caterpillar-induced...
Transgenic plants were produced that expressed a wide range of randomly chosen sequences the tripartite tomato spotted wilt virus (TSWV) RNA genome or its complement. Testing progenies these revealed only transgenic expression N NS(M) gene resulted in resistance to TSWV.
Transgenic tobacco plants have been obtained expressing nucleoprotein (N) gene sequences of three different tospoviruses known to affect vegetable crops: tomato spotted wilt virus (TSWV), chlorotic spot (TCSV), and groundnut ringspot (GRSV). The chimeric plant transformation vector used comprised the viral N sequences, each with a copy CaMV 35S promoter nos terminator. Despite high levels homology between (74-82%) presence repeated terminator in this construct, unrearranged copies triple...