A5066711727- Probiotics and Fermented Foods
- Microbial Metabolites in Food Biotechnology
- Bacterial Genetics and Biotechnology
- Enzyme Production and Characterization
- RNA and protein synthesis mechanisms
- Pneumonia and Respiratory Infections
- Bacteriophages and microbial interactions
- Gut microbiota and health
- Antibiotic Resistance in Bacteria
- Food composition and properties
- Microbial Metabolic Engineering and Bioproduction
- Pneumocystis jirovecii pneumonia detection and treatment
- DNA and Nucleic Acid Chemistry
- Amino Acid Enzymes and Metabolism
- Protein Hydrolysis and Bioactive Peptides
- Polyamine Metabolism and Applications
- Antimicrobial Resistance in Staphylococcus
- Biopolymer Synthesis and Applications
- Aquaculture disease management and microbiota
- Biofuel production and bioconversion
- Infant Nutrition and Health
- Bacterial Infections and Vaccines
- Helminth infection and control
- Microbial Metabolism and Applications
- Streptococcal Infections and Treatments
Centro de Investigaciones Biológicas Margarita Salas
2015-2024
Consejo Superior de Investigaciones Científicas
2010-2023
Tecnológico de Monterrey
2019
Junta de Andalucía
2017
Unidades Centrales Científico-Técnicas
2014
San Francisco State University
2004
University of Castilla-La Mancha
1996
Brookhaven National Laboratory
1981-1995
Universidad de León
1991-1994
Casa de Velázquez
1986
Probiotics, prebiotics and synbiotics are frequently-used components for the elaboration of functional food. Currently, most commercialized probiotics limited to a few strains genera Bifidobacteria, Lactobacillus Streptococcus, which produce exopolysaccharides (EPS). This suggests that beneficial properties these microorganisms may be related biological activities biopolymers. In this work we report 2-substituted-(1,3)-β-D-glucan non-dairy bacterial origin has prebiotic effect on three...
A chromosomal gene of Streptococcus pneumoniae carrying a spontaneous mutation to sulfonamide resistance was identified. Comparison its DNA sequence with the wild-type showed that mutation, sul-d, consisted an insert 6 base pairs, repeat adjacent 6-base-pair segment. The encoded 34-kilodalton polypeptide, SulA, which as dimer or trimer constituted enzyme dihydropteroate synthase. This shown by activity measurements, expression in minicells Bacillus subtilis, and amino-terminal polypeptide...
A system for molecular cloning in Streptococcus pneumoniae was developed. The multicopy plasmids pMV158 (5.4 kilobases) and pLS1 (4.3 kilobases), which confer tetracycline resistance, were used as vectors to clone chromosomal genes of S. host cells this species. 3.3-kilobase restriction fragment containing the malM gene, codes amylomaltase, cloned a deletion mutant lacking homology with fragment. recombinant plasmid pLS70, could transform over 50% recipient population maltose utilization....
The YycFG two-component system, originally identified in Bacillus subtilis, is highly conserved among gram-positive bacteria with low G+C contents. In Streptococcus pneumoniae, the YycF response regulator has been reported to be essential for cell growth, but signal which it responds and gene members of regulon remain unclear. order investigate role S. we increased expression yycF by using a maltose-inducible vector analyzed genome-wide effects on transcription protein during course...
Exopolysaccharides have prebiotic potential and contribute to the rheology texture of fermented foods. Here we analyzed in vitro bioavailability immunomodulatory properties 2-substituted (1,3)-beta-D-glucan-producing bacterium Pediococcus parvulus 2.6. It resists gastrointestinal stress, adheres Caco-2 cells, induces production inflammation-related cytokines by polarized macrophages.
The frequency of plasmid establishment in the transformation Streptococcus pneumoniae by DNA was increased more than 10-fold when carried homologous to host chromosome. Perfect homology not necessary for such facilitation; small additions or deletions were tolerated, but extensive segment either chromosome reduced eliminated facilitation. facilitated transfer showed a linear dependence on monomeric concentration rather quadratic found absence homology, which indicated that entering fragments...
Abstract Six lactic acid bacteria (LAB) isolated from Algerian sheep’s milk, traditional butter, date palm sap and barley, which produce dextran, mannitol, oligosaccharides vitamin B 2 have been characterized. They were identified as Leuconostoc mesenteroides (A4X, Z36P, B12 O9) Liquorilactobacillus mali (BR201 FR123). Their exopolysaccharides synthesized sucrose by dextransucrase (Dsr) characterized dextrans with (1,6)- d -glucopyranose units in the main backbone branched at positions O -4,...
ABSTRACT The Bacillus subtilis des gene encodes the cold-inducible Δ5 lipid desaturase involved in formation of unsaturated fatty acids from saturated phospholipid precursors. Here, we describe expression pattern response to a temperature downshift 37 20°C. We found that synthesis mRNA is undetectable at 37°C but dramatically induced upon downshift. Decay characteristics transcript as well vivo decay B. bulk were investigated. results showed stability lasted substantially longer 20°C than...