A5009941211- Neuroscience and Neuropharmacology Research
- Glioma Diagnosis and Treatment
- Pancreatic and Hepatic Oncology Research
- Pancreatitis Pathology and Treatment
- Cytokine Signaling Pathways and Interactions
- Colorectal Cancer Treatments and Studies
- Mitochondrial Function and Pathology
- Radiation Therapy and Dosimetry
- ATP Synthase and ATPases Research
- Genetics, Aging, and Longevity in Model Organisms
- Neuroscience and Neural Engineering
- Cancer, Hypoxia, and Metabolism
- Melanoma and MAPK Pathways
- Protein Tyrosine Phosphatases
- Effects of Radiation Exposure
- Metabolism and Genetic Disorders
- Planarian Biology and Electrostimulation
- Muscle activation and electromyography studies
- PI3K/AKT/mTOR signaling in cancer
- Single-cell and spatial transcriptomics
- Liver Disease Diagnosis and Treatment
- Radiation Effects in Electronics
- Frailty in Older Adults
- Growth Hormone and Insulin-like Growth Factors
- Ion channel regulation and function
University of Rostock
2015-2024
Universitätsmedizin Rostock
2014-2023
Semmelweis University
1989-1990
Epileptic seizures are frequent in patients with glioblastoma, and anticonvulsive treatment is often necessary. While clinical guidelines recommend all approved anticonvulsants, so far it still unclear which of the available drugs best therapeutic option for treating glioma-associated seizures, also view possible anti-tumorigenic effects. In our study, we employed four patient-derived low-passage cell lines glioblastoma three brain metastases, challenged these cultures anticonvulsants...
Abstract Aim Mitochondrial DNA (mtDNA) mutations can negatively influence lifespan and organ function. More than 250 pathogenic mtDNA are known, often involving neurological symptoms. Major neurodegenerative diseases share key etiopathogenetic components ie mutations, mitochondrial dysfunction oxidative stress. Methods Here, we characterized a conplastic mouse strain (C57BL/6 J‐mtNOD) carrying an electron transport chain complex IV mutation that leads to altered cytochrome c oxidase subunit...
Interferon-gamma (IFNγ) is a multifunctional cytokine with antifibrotic and antiproliferative efficiency. We previously found that pancreatic stellate cells (PSC), the main effector in cancer-associated fibrosis, are targets of IFNγ action pancreas. Applying combined experimental computational approach, we have demonstrated pivotal role STAT1 signaling PSC. Using vivo vitro models cancer, now studied effects on tumor themselves. hypothesize inhibits progression through two mechanisms,...
AIMTo study the molecular effects of three different vitamin D2, D3 and calcipotriol, in pancreatic stellate cells (PSCs). METHODSQuiescent PSCs were isolated from mouse pancreas activated vitro by seeding on plastic surfaces.The exposed to D-vitamins as primary cultures (early-activated PSCs) upon re-culturing (fullyactivated cells).Exhibition A-containing lipid droplets was visualized oil-red staining.Expression α-smooth muscle actin (α-SMA), a marker PSC activation, monitored...
The present work exemplifies how parameter identifiability analysis can be used to gain insights into differences in experimental systems and uncertainty estimates handled. case study, presented here, investigates interferon-gamma (IFNγ) induced STAT1 signalling two cell types that play a key role pancreatic cancer development: stellate cells. IFNγ inhibits the growth for both of cells may prototypic agents simultaneously hit stroma We combined time-course experiments with mathematical...
An abnormal glutamate signaling of glioblastoma may contribute to both tumor progression and the generation glioma-associated epileptic seizures. We hypothesized that AMPA receptor antagonist perampanel (PER) could attenuate growth events. F98 glioma cells, grown orthotopically in Fischer rats, were employed as a model investigate therapeutic efficiency PER (15 mg/kg) adjuvant standard radiochemotherapy (RCT). The epileptiform phenotype was investigated by video-EEG analysis field potential...
Low-passage cancer cell lines are versatile tools to study tumor biology. Here, we have employed four such lines, established from primary tumors of colorectal (CRC) patients, evaluate effects the small molecule kinase inhibitors (SMI) vemurafenib, trametinib, perifosine, and regorafenib in an vitro setting. The mutant BRAF (V600E/V600K) inhibitor but also MEK1/2 trametinib efficiently inhibited DNA synthesis, signaling through ERK1/2 expression genes downstream cells only. In case AKT three...
Reactive oxygen species (ROS) have been implicated in the pathogenesis of acute pancreatitis (AP) for many years but experimental evidence is still limited. Uncoupling protein 2 (UCP2)-deficient mice are an accepted model age-related oxidative stress. Here, we analysed how UCP2 deficiency affects severity AP young and older (3 12 months old, respectively) triggered by up to 7 injections secretagogue cerulein (50 μg/kg body weight) at hourly intervals. Disease was assessed time points from 3...
AIM:To gain insights into the molecular action of erlotinib in pancreatic cancer (PC) cells. METHODS:Two PC cell lines, BxPC-3 and Capan-1, were treated with various concentrations erlotinib, specific mitogen-activated protein kinase (MEK) inhibitor U0126, B (AKT) XIV.DNA synthesis was measured by 5-bromo-2'-deoxyuridine (BrdU) assays.Expression phosphorylation epidermal growth factor receptor (EGFR) downstream signaling molecules quantified Western blot analysis.The data processed to...
Patient-derived tumor cell lines are a powerful tool to analyze the sensitivity of individual tumors specific therapies in mice. An essential prerequisite for such an approach reliable quantitative techniques monitor progression vivo.We have employed HROC24 cells, grown heterotopically NMRI Foxn1nu mice, as model microsatellite instable colorectal cancer investigate therapeutic efficiencies 5'-fluorouracil (5'-FU) and mutant BRAF inhibitor PLX4720, vemurafenib analogue, by three independent...
Galvanotaxis, the migration along direct current electrical fields, may contribute to invasion of brain cancer cells in tumor-surrounding tissue. We hypothesized that pharmacological perturbation epidermal growth factor (EGF) receptor and downstream phosphatidylinositol 3-kinase (PI3K)/AKT pathway prevent galvanotactic migration. In our study, patient-derived glioblastoma metastases were exposed field conditions. Velocity direction estimated. To determine effects EGF antagonist afatinib AKT...
To establish patient-individual tumor models of rectal cancer for analyses novel biomarkers, individual response prediction and therapy regimens.Establishment cell lines was conducted by direct in vitro culturing vivo xenografting with subsequent culturing. Cell were in-depth characterized concerning morphological features, invasive migratory behavior, phenotype, molecular profile including mutational analysis, protein expression, confirmation origin DNA fingerprint. Assessment...
Large-scale, heteroplasmic and generally pathogenic mtDNA defects (as induced by defective mitochondrial DNA polymerase, clonal mutations or deletions) are known to negatively impact on life span can result in apoptosis tissue loss in, e.g., skeletal muscle reduce learning abilities. The functional of homoplasmic specific point mutations, genes coding for the electron transport chain, however, remains a matter debate. present study contributes this discussion provides evidence that single...
Introduction Ageing-related processes such as cellular senescence are believed to underlie the accumulation of diseases in time, causing (co)morbidity, including cancer, thromboembolism and stroke. Interfering with these may delay, stop or reverse morbidity. The aim this study is investigate link between (co)morbidity ageing by exploring biomarkers molecular mechanisms disease-triggered deterioration patients pancreatic ductal adenocarcinoma (PDAC) (thromboembolic) ischaemic stroke (IS)....
Microbeam radiotherapy could help to cure malignant tumours which are currently still considered therapy-resistant. With an irradiation target in the thoracic cavity, heart would be one of most important organs at risk. To assess acute adverse effects microbeam heart, a powerful ex vivo tool was created by combining Langendorff model isolated beating mammalian with X-Tream dosimetry. In first pilot experiment conducted Biomedical and Imaging Beamline Australian Synchrotron, system tested...
Glioblastoma is the most common and aggressive primary brain tumor. Multiple genetic epigenetic alterations in several major signaling pathways-including phosphoinositide 3-kinases (PI3K)/AKT/mTOR Raf/MEK/ERK pathway-could be found. We therefore aimed to investigate biological molecular effects of small-molecule kinase inhibitors that may interfere with those pathways. For this purpose, patient-derived glioblastoma cells were challenged dactolisib, ipatasertib, MK-2206, regorafenib, or...
Several cues for a directional migration of colorectal cancer cells were identified as being crucial in tumor progression. However, galvanotaxis, the direct-current electrical fields, has not been investigated so far. Therefore, we asked whether fields could be used to mobilize along field vectors. For this purpose, five patient-derived low-passage cell lines exposed strengths 150-250 V/m vitro, and vectors was investigated. To further study role voltage-gated calcium channels on...