A5086036636- Diet and metabolism studies
- Pancreatic function and diabetes
- Metabolism, Diabetes, and Cancer
- Ion Transport and Channel Regulation
- Erythrocyte Function and Pathophysiology
- Diet, Metabolism, and Disease
- Diabetes Treatment and Management
- Gastrointestinal motility and disorders
- Cancer, Hypoxia, and Metabolism
- Iron Metabolism and Disorders
- Protein Structure and Dynamics
- Amino Acid Enzymes and Metabolism
- Clinical Nutrition and Gastroenterology
- Lipid Membrane Structure and Behavior
- Metabolism and Genetic Disorders
- Helicobacter pylori-related gastroenterology studies
- Bariatric Surgery and Outcomes
- Digestive system and related health
- Barrier Structure and Function Studies
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Vitamin C and Antioxidants Research
- Hormonal Regulation and Hypertension
- Drug Transport and Resistance Mechanisms
- Reproductive System and Pregnancy
- Diabetes Management and Research
King's College London
2015-2025
British Heart Foundation
2015-2023
King's College School
2013-2018
Institut de Radioprotection et de Sûreté Nucléaire
2000
King's College - North Carolina
1997
Harvard University
1996
New England Baptist Hospital
1996
Parker Hannifin (United States)
1996
Deaconess Hospital
1996
University of Urbino
1995
On a three-dimensional templated model of GLUT1 (Protein Data Bank code 1SUK), molecular recognition program, AUTODOCK 3, reveals nine hexose-binding clusters spanning the entire "hydrophilic" channel. Five these cluster sites are within 3-5 Å 10 glucose transporter deficiency syndrome missense mutations. Another three 8 two other d-Glucose binds to five in external channel opening, with increasing affinity toward pore center and then passes via narrow into an internal vestibule containing...
Transmembrane glucose transport, facilitated by transporters (GLUTs), is commonly understood through the simple mobile carrier model (SMCM), which suggests that central binding site alternates exposure between inside and outside of cell, facilitating exchange. An alternative "multisite model" posits transport a stochastic diffusion process ligand-operated gates within transporter's channel. This study aims to test these models conducting atomistic molecular dynamics simulations multiple...
Ron Whittam had a distinguished academic career in the field of cellular physiology and biochemistry, significantly study transmembrane ion transport mechanisms. His work spanned several decades institutions including universities Manchester, Sheffield, Cambridge, Oxford Leicester. He served Royal Air Force from 1943 to 1947. Undergraduate success was followed by various prestigious fellowships positions. held founding Chair General Physiology at Leicester University. research addressed an...
This study investigates the effects of androgens, antiandrogen flutamide and green tea catechins on glucose transport inhibition in human erythrocytes. These may relate to antidiabetogenic tea. Testosterone, 4‐androstene‐3,17‐dione, dehydroepiandrosterone (DHEA) DHEA‐3‐acetate inhibit exit from erythrocytes with half‐maximal inhibitions ( K i ) 39.2±8.9, 29.6±3.7, 48.1±10.2 4.8±0.98 μ M , respectively. The competitively relieves these phloretin. Dehydrotestosterone has no effect transport,...
Water flows generated by osmotic and hydrostatic pressure electrical currents were measured in sheets of isolated rabbit ileum at 20 degrees C. Flows across the mucosal serosal surfaces monitored continuously simultaneous measurement tissue volume change (with an optical lever) net water one surface a capacitance transducer). Osmotic gradients imposed separately, using probe molecules various sizes from ethanediol (68 Da) to dextrans (161 000 Da). each elicited with very short delay. The...
1. Confocal microscopic studies of rat colonic mucosa showed that the pericryptal sheath surrounding distal crypts is an effective barrier both to dextran and NaCl movement, whereas no such structure surrounds caecal crypts. 2. The was functionally demonstrated by accumulation Sodium Green within space. After exposure benzamil, decreased. Fluorescein isocyanate-labelled (FITC dextran; molecular mass 10000 Da) accumulated in crypt lumens spaces. Both were absent from zone 3. Low dietary Na+...
1. To test whether colonic crypts are secretory or absorptive interstitial [Na+] in rat descending mucosa is determined using video-enhanced imaging of the impermeant acid form fluorescent Na+ probe SBFI (Molecular Probes) and intracellular monitored with (AM form). In perifused isotonic Tyrode solution = 500-650 mM. Following exposure to containing theophylline (10 mM) falls by 300-450 mM within 1 min. Exposure amiloride (0.2 reduces from ca 25 12 15 min concurrently decreases fluid...
17 beta-Oestradiol (ED when subscript to K) and the phytoestrogen isoflavone genistein (GEN) inhibit glucose transport in human erythrocytes erythrocyte ghosts. The selective oestrogen receptor modulators or anti-oestrogens, faslodex (ICI 182780) (FAS) tamoxifen (TAM), competitively antagonize oestradiol inhibition of exit from (K(i(ED/FAS))=2.84+/-0.16 microM K(i(ED/TAM))=100+/-2 nM). Faslodex has no significant inhibitory effect on exit, but alone inhibits (K(i(TAM))=300+/-100 In ghosts,...
Carrier-mediated water cotransport is currently a favored explanation for movement against an osmotic gradient. The vestibule within the central pore of Na+-dependent cotransporters or GLUT2 provides necessary precondition mechanism, explaining this phenomenon without carriers. Simulating equilibrative glucose inflow via narrow external orifice raises vestibular tonicity relative to solution. Vestibular hypertonicity causes inflow, which hydrostatic pressure and forces water, salt, into...
1. Theophylline (10 mM) and choleragen change the direction of net Cl‐ movements across rabbit ileum, in short‐circuit current condition, from absorption to secretion. The specific activity ratio R tracers within tissue coming mucosal serosal solutions respectively is increased, which consistent with an increase exchange flux border. 2. Net Na+ movement also changed secretion by theophylline choleragen; raised theophylline. Because large paracellular component transepithelial movements,...