A5038167056- Hippo pathway signaling and YAP/TAZ
- Cellular Mechanics and Interactions
- Skin and Cellular Biology Research
- Microtubule and mitosis dynamics
- Wnt/β-catenin signaling in development and cancer
- Cancer-related gene regulation
- Exercise and Physiological Responses
- Muscle Physiology and Disorders
- Muscle metabolism and nutrition
- Cell Adhesion Molecules Research
- Adipose Tissue and Metabolism
- Cancer-related Molecular Pathways
- Vitamin D Research Studies
Cologne Excellence Cluster on Cellular Stress Responses in Aging Associated Diseases
2016-2024
University of Cologne
2016-2023
Melanoma, an aggressive skin malignancy with increasing lifetime risk, originates from melanocytes (MCs) that are in close contact surrounding epidermal keratinocytes (KCs). How the microenvironment controls melanomagenesis remains poorly understood. In this study, we identify unexpected non-cell autonomous role of polarity proteins, molecular determinants cytoarchitecture, malignant melanoma. Epidermal Par3 inactivation mice promotes MC dedifferentiation, motility, and hyperplasia and,...
Abstract Epithelial homeostasis requires balanced progenitor cell proliferation and differentiation, whereas disrupting this equilibrium fosters degeneration or cancer. Here we studied how polarity signaling orchestrates epidermal self-renewal differentiation. Using genetic ablation, quantitative imaging, mechanochemical reconstitution atomic force microscopy, find that mammalian Par3 couples genome integrity fate through shaping keratinocyte mechanics, rather than mitotic spindle...
The polarity proteins Par3 and aPKC are key regulators of processes altered in cancer. Par3/aPKC thought to dynamically interact with Par6 but increasing evidence suggests that also exert complex-independent functions. Whereas aPKCλ serves as tumor promotor, can either promote or suppress tumorigenesis. Here we asked whether how genetically control two-stage skin carcinogenesis. Epidermal loss Par3, aPKCλ, both, strongly reduced multiplicity increased latency inhibited invasion similar...
Morphological studies of skeletal muscle tissue provide insights into the architecture fibers, surrounding cells, and extracellular matrix (ECM). However, a spatial proteomics analysis including muscle-tendon transition zone is lacking. Here, we prepare cryotome sections mouse soleus measure each slice using short liquid chromatography-mass spectrometry (LC-MS) gradients. We generate 3,000 high-resolution protein profiles that serve as basis for network to reveal complex junction. Among...
ABSTRACT Epithelial homeostasis requires balanced progenitor cell proliferation and differentiation, whereas disrupting this equilibrium fosters degeneration or cancer. Here we studied how polarity signaling orchestrates epidermal self-renewal differentiation. Using genetic ablation, quantitative imaging, mechanochemical reconstitution atomic force microscopy, find that mammalian Par3 couples genome integrity fate through shaping keratinocyte mechanics, rather than mitotic spindle...
Abstract Phosphorylation of the myosin regulatory light chain 2 is a post-translational modification commonly used to report for ATPase activity and actomyosin contractility. While its use in cell culture has been broadly reported several studies, data on immunostaining tissues sparse inconsistent. In this protocol we methodology stain phospho-myosin (pMLC2 Ser19) double (ppMLC2Thr18/ adult murine epidermis.
Abstract Dephosphorylation of epitopes is a common way testing phospho-specificity antibodies. Whereas it regularly used protocol for dephosphorylation in cell lysates, its use immunohistochemistry has been limited. Here we describe methodology to dephosphorylate adult murine tissues by using Antarctic Phosphatase, an alkaline phosphatase. This compatible with subsequent immunohistochemistry, making suitable validating phospho-immunoreactivity tissues.
Background: Smooth muscle cells (SMCs) are a key factor in the development of various cardiovascular diseases. It is known that they can change their phenotype response to external stimuli into macrophage-like and create proinflammatory environment, which could be trigger for In vitro experiments with SMCs should help clarify molecular mechanisms behind these observations. However, primary tend dedifferentiate after time under 2D conditions. Hence, this study we cultivated 3D culture...