A5065833472- Methane Hydrates and Related Phenomena
- Seismic Waves and Analysis
- Earthquake Detection and Analysis
- Geophysical Methods and Applications
- Monoclonal and Polyclonal Antibodies Research
- Peptidase Inhibition and Analysis
- Cancer-related Molecular Pathways
- HIV Research and Treatment
- vaccines and immunoinformatics approaches
- Cancer, Hypoxia, and Metabolism
- Immunotherapy and Immune Responses
- Ubiquitin and proteasome pathways
- Cancer Immunotherapy and Biomarkers
- Immune Cell Function and Interaction
- Ocular Oncology and Treatments
- DNA and Nucleic Acid Chemistry
- Glycosylation and Glycoproteins Research
- interferon and immune responses
- HIV/AIDS drug development and treatment
- Fluorine in Organic Chemistry
- Tissue Engineering and Regenerative Medicine
- Galectins and Cancer Biology
- Genomics and Chromatin Dynamics
- Microtubule and mitosis dynamics
- Inorganic Chemistry and Materials
Ansun BioPharma (United States)
2024
Amgen (United States)
2023
Fred Hutch Cancer Center
2019-2021
University of California, Santa Cruz
2013-2017
University of Wisconsin–Madison
2010
The elicitation of broadly neutralizing antibodies (bNAbs) against the HIV-1 envelope glycoprotein (Env) trimer remains a major vaccine challenge. Most cross-conserved protein determinants are occluded by self-N-glycan shielding, limiting B cell recognition underlying polypeptide surface. exceptions to contiguous glycan shield include conserved receptor CD4 binding site (CD4bs) and (gp)41 elements proximal furin cleavage site. Accordingly, we performed heterologous trimer-liposome...
The DREAM complex represses cell cycle genes during quiescence through scaffolding MuvB proteins with E2F4/5 and the Rb tumor suppressor paralog p107 or p130. Upon entry, dissociates from p107/p130 recruits B-Myb FoxM1 for up-regulating mitotic gene expression. To understand biochemical mechanisms underpinning function regulation, we investigated structural basis assembly. We identified a sequence in component LIN52 that binds directly to pocket domains of p130 when phosphorylated on DYRK1A...
We report the generation of a novel anti-LAG-3/TIGIT bispecific IgG4 antibody, ZGGS15, and evaluated its anti-tumor efficacy in mouse models as monotherapy or combination with PD-1 antibody. ZGGS15 exhibited strong affinities for human LAG-3 TIGIT, KDs 3.05 nM 2.65 nM, respectively. has EC50s 0.69 1.87 binding to TIGIT on CHO-K1 cells, competitively inhibited MHC-II (IC50 = 0.77 nM) CD155 0.24 nM). does not induce ADCC, CDC, obvious cytokine production. In vivo results showed that had better...
Significance The retinoblastoma (Rb) pocket protein and E2F transcription factor families regulate cell division are commonly deregulated in proliferating cancer cells. An important question has been what distinguishing molecular features of Rb its interaction with result unique potency as a tumor suppressor relative to homologous proteins p107 p130. Here we identify structures Rb, p107, E2Fs that determine the specificity their association. We explain binding preferences an X-ray crystal...
Understanding the molecular mechanisms by which antibodies target and neutralize HIV-1 envelope glycoprotein (Env) is critical in guiding immunogen design vaccine development aimed at eliciting cross-reactive neutralizing (NAbs). Here, we analyzed monoclonal (mAbs) isolated from non-human primates (NHPs) immunized with variants of a native flexibly linked (NFL) Env stabilized trimer derived tier 2 clade C 16055 strain. The displayed activity against autologous virus potencies ranging 0.005...
Abstract ZG005 is a humanized anti-PD-1/TIGIT bispecific antibody that effectively blocks the binding between PD-1 and TIGIT, on immune cells, with their specific ligands PD-L1 PVR, tumors. As dual blockade of PD-1/TIGIT checkpoints, exhibits sustained occupancy to both targets inhibits pathways specifically simultaneously, leading synergistic effects boosting ability system in killing tumor cells. With high affinities, elicits activation T cells NK resulting increased release cytokines...
Abstract Understanding the molecular mechanisms by which antibodies target and neutralize HIV-1 envelope glycoprotein (Env) is critical in guiding immunogen design vaccine development aimed at eliciting cross-reactive neutralizing (NAbs). Here, we analyzed monoclonal (mAbs) isolated from non-human primates (NHPs) immunized with variants of a native flexibly linked (NFL) Env stabilized trimer derived tier 2 clade C 16055 strain. The displayed activity against autologous virus potencies...
Abstract ZGGS18 is a bifunctional fusion protein targeting both human vascular endothelial growth factor (VEGF) and transforming factor-β (TGF-β), which consist of monoclonal antibody against VEGF an engineered extracellular domain TGF-β receptor II (TGF-βRII ECD). In vitro studies show specifically simultaneously binding towards various VEGFs capturing different isoforms by trap domains that play synergistic role to block signaling VEGF/TGF-β with their receptors. For instance, inhibits...
Uracil DNA glycosylases (UDGs) catalyze the removal of uracil bases that arise in by spontaneous cytosine deamination and dUTP misincorporation polymerases. UDGs are found all kingdoms life many cases have been shown to interact functionally with single‐strand DNA‐binding proteins (SSBs). The structural mechanisms govern UDG/SSB interactions vivo significance complex currently unknown. We present X‐ray crystal structures Escherichia coli UDG 9 C‐terminal residues E. SSB Deinococcus...