In this study, the ultrasound-assisted extraction (UAE) conditions of flavonoids from Lactuca indica L.cv. Mengzao (LIM) leaves was optimized, and content their antioxidant potential in different parts were analyzed. The optimal parameters to obtain highest total (TFC) a ratio liquid solid 24.76 mL/g, ultrasonic power 411.43 W, ethanol concentration 58.86% an time 30 min, average TFC LIM could reach 48.01 mg/g. For yield flavonoids, UAE method had best capacity compared with solvent...

Silage of native grasses can alleviate seasonal forage supply imbalance in pastures and provide additional sources to meet demand. The study aimed investigate the effects Lactobacillus plantarum (LP), buchneri (LB), combination with (PB) on nutritional quality, fermentation microbial community grass silage at 2, 7, 15, 60 days after ensiling 4 8 aerobic exposure. results showed that dry matter content, crude protein number lactic acid bacteria, acetic content increased pH ammonia nitrogen...

Selenium promotes plant growth and improves nutritional quality, the role of nano-selenium in alfalfa regulating quality is unknown. In this study, using 15N labeling method, it was found that could promote nitrogen metabolism photosynthesis by increasing light energy capture capacity activities key enzymes process, leading to an increase accumulation dry matter content. The transcriptome metabolome revealed mainly affected pathways 'biosynthesis amino acids', 'starch sucrose metabolism',...

Better quality and odor of silage normal microbial fermentation metabolism are mostly dependent on an appropriate moisture content. The purpose this study was to determine the effects different content gradients (50, 60, 70, 80%) bacterial community, odor, alfalfa at 60 days by using gas chromatography-mass spectrometry (GC-MS) electronic nose, with six replicates per group. results showed that there were significant differences in response intensity among all groups, which 80% group had...

ABSTRACT Native grasses possess rich diversity and contribute to enhancing the nutritional value of silage, promoting digestion absorption, thus improving health livestock such as cattle sheep. However, in northern China, silage fermentation process occurs at relatively low temperatures, necessitating use cold-tolerant lactic acid bacteria (LAB). This study examined effect Pediococcus acidilactici (L10), a strain selected for its low-temperature tolerance, added native grass 5°C (LT), 15°C...

L. cv. Mengzao, a medicinal plant of the Ixeris genus, is rich in flavonoids. In order to thoroughly analyze distribution and dynamic change major flavonoids its various parts from different growth periods, extracted

The objective of this study was to determine the effect Lactobacillus plantarum (L. plantarum) and propionic acid (PA) on microbial community fermentation performance high-moisture amaranth silage.Amaranth silages were rown without addition (AhGCK) as a control with L. JYLP-002 (AhGLP) or (AhGPA) then opened after 60 days ensiling quality.Crude protein (CP) content, lactic (LA) bacteria (LAB) counts significantly higher in AhGLP AhGPA compared those AhGCK (p < 0.05). In contrast, pH, acetic...

Harvest time and storage had significant effects on nutrient quality Volatile Organic Compounds (VOCs) of alfalfa. The objective this study was to use headspace solid-phase microextraction gas chromatography-mass spectrometry (HS-SPME-GC-MS) analyze alfalfa at different harvest periods (budding stage, early blooming, full blooming), for 0 d, 90 180 270 360 the dynamic changes in VOCs nutritional quality. Results indicated that ketones, aldehydes, alcohols esters were main volatile components...

In this study, the triterpenoids in leaves of Lactuca indica L.cv. Mengzao (LIM) were extracted via microwave-assisted ethanol extraction, and optimum extraction conditions for determined through single-factor experiments Box–Behnken method. The effects three factors (solid–liquid ratio, microwave power time) on total content (TTC) evaluated. TTC different parts (roots, stems, flowers) LIM growth stages was studied, scavenging highest DPPH, ABTS hydroxyl free radicals investigated. results...

Objective The objective of this study was to isolate lactic acid bacteria (LAB) from native grasses and naturally fermented silages, determine their identity, assess effects on silage quality bacterial communities the three steppe types for 60 days. Methods Among 58 isolated LAB strains, Limosilactobacillus fermentum (BL1) Latilactobacillus graminis (BL5) were identified using 16S rRNA sequences. Both strains showed normal growth at 15- 45°C temperature, 3-6.5% NaCl concentration, pH 4-9....

This study aimed to analyze the fermentation quality, microbial community, and volatile metabolites of oat silage harvested at two different stages, while examining correlation between microorganisms metabolites.Oats were growth stages (pre-heading [PRH] post-heading [POH] stages), followed by 90 days natural fermentation, with 6 replicates per treatment. Pre- post-silage samples randomly selected for nutrient composition, parameters, population, high-throughput sequencing analysis. Volatile...

In this study, alfalfa silage with different packing densities (500 kg/m3, 600 kg/m3 and 700 kg/m3) were set up under outdoor high temperature indoor normal temperature. The chemical composition, fermentation quality microbial community of analyzed. results showed that DM WAC content decreased the increase density. When density is 600kg/m3, CP reaches maximum ADF lowest. Temperature has a certain effect on silage. Lactobacillus plantarum fermented at lower than At same time, However, was...

Objective The aim of this study was to investigate the effects Lactiplantibacillus plantarum ( L. ) and propionic acid (PA) on fermentation characteristics microbial community amaranth Amaranthus hypochondriaus silage with different moisture contents. Methods Amaranth harvested at maturity stage prepared for ensiling. There were two content gradients (80%: AhG, 70%: AhS; fresh material: FM) three treatments (control: CK, : LP, acid: PA) set up, silages opened after 60 d Results results...

Hydrothermal biochar has demonstrated potential in enhancing crop growth by improving soil properties and microbial activity; however, its effectiveness varies with application rate, excessive amounts potentially inhibiting plant growth. This study employed a pot experiment approach to compare varying rates of hydrothermal (ranging from 0 50 t/ha) analyze effects on alfalfa biomass, photosynthetic efficiency, nutrient content, community composition. Biochar increased dry weight 12.22-21.20%...

This study aimed to screen and identify lactic acid bacteria (LAB) strains from the Stipa grandis naturally fermented silage, assess their effects on silage quality bacterial community of after 60 days fermentation process. A total 38 LAB were isolated, ZX301 YX34 identified as Lactiplantibacillus plantarum Pediococcus pentosaceus using 16S rRNA sequences; they can normally grow at 10−30 °C, with a tolerance pH NaCl 3.5 8.0 3 6.5%, respectively. Subsequently, two isolated one commercial...

Lactuca indica L. cv. Mengzao (LIM), acknowledged as a pivotal "One Root of Medicine and Food", boasts dual applications in both culinary medicinal domains. This research delves into the influence various harvest periods (vegetative, budding, blossom, fruiting) on distinct parts (roots, stems, leaves, flowers, seeds) LIM, employing plant metabolomics to assess its chemical constituents. A total 66 constituents were identified with 11 components emerging potential markers for distinguish...

Abstract Background The objective was to determine the effects of different lactic acid bacteria (LAB) on amaranth ( Amaranthus hypochondriaus ) silage fermentation characteristics, changes in bacterial communities and their functional properties, investigate preferential mechanisms action Limosilactobacillus fermentum L. Latilactobacillus graminis silage. LAB were screened identified from native grasses its naturally fermented silage, performance community after 60 days investigated. BL1...

Lactuca indica L. contains high amounts of flavonoids and phenolic acids. However, there is limited information on the composition these compounds in different parts plant. The present study analyzed secondary metabolite profiles stem, leaf, flower, seed indicaL.cv. Mengzao (LIM) using a widely targeted metabolomic approach. A total 576 metabolites were identified, including 218, 267, 232, 286, 302, 308 differentially accumulated (DAMs) stem_vs_leaf, stem_vs_flower, stem_vs_seed,...

Spirulina (Arthrospira platensis) is rich in a variety of fermentable fibers and prebiotics, which can promote the proliferation beneficial flora intestinal tract ruminants optimize balance microorganisms rumen. The aim this study was to evaluate whether dietary supplementation with has effect on rumen microbial community serum indices lambs. For purpose, 36 lambs mean weight 21.68 kg (standard deviation 1.04 kg) an age approximately 5 months 4 days) were selected for study. same scale used...

Abstract Aims The aim of this study was to investigate the dynamics bacterial communities and natural fermentation quality in three steppe types [meadow (MS); typical (TS); desert (DS)] on Mongolian Plateau. Methods results PacBio single molecule with real-time sequencing technology applied provide insights into physicochemical characteristics complex microbiome native grass after 1, 7, 15, 30 days process. dry matter, crude protein, water soluble carbohydrate (WSC) contents groups slowly...

Lactuca indica L. cv. Mengzao (LIM) has both edible and medicinal uses. Its leaves is used as vegetables, but the whole plant important value. In order to explore effects of different harvest periods on parts LIM, chemical constituents LIM were evaluated using ultrahigh-performance liquid chromatography coupled with quadrupole tandem Q exactive mass spectrometry (UPLC-QE-MS/MS). 66 detected, 21 components identified potential markers distinguish LIM. Furthermore, sensitive UPLC-MS/MS method...

Lactuca indica L. cv. Mengzao (LIM) is classified as one of the “One Root Medicine and Food”, it has both edible medicinal uses. To exploring effects in different harvest periods on parts LIM, chemical constituents LIM were evaluated based plant metabolomics. 66 detected, 11 components identified potential markers to distinguish parts. Among nutritional organs contain highest levels cichoric acid, rutin chlorogenic acid. Particularly, leaves at budding stage chicoric acid content 11.70 mg...