We report the synthesis of highly luminescent, water soluble quantum clusters (QCs) gold, which are stabilized by an iron binding transferrin family protein, lactoferrin (Lf). The synthesized AuQC@Lf were characterized using UV-Visible spectroscopy, X-ray photoelectron spectroscopy (XPS), transmission electron microscopy (TEM), photoluminescence (PL), matrix assisted laser desorption ionization mass spectrometry (MALDI-MS), FTIR and circular dichroism (CD) along with picosecond-resolved...

The synthesis of a luminescent quantum cluster (QC) gold with yield approximately 4 % is reported. It was synthesized in gram quantities by the core etching mercaptosuccinic acid protected nanoparticles bovine serum albumin (BSA), abbreviated as Au(QC)@BSA. characterized and Au(38) assigned tentatively from mass spectrometric analysis. Luminescence QC exploited "turn-off" sensor for Cu(2+) ions "turn-on" glutathione detection. Metal-enhanced luminescence (MEL) this presence silver...

A novel interfacial route has been developed for the synthesis of a bright-red-emitting new subnanocluster, Au(23), by core etching widely explored and more stable cluster, Au(25)SG(18) (in which SG is glutathione thiolate). slight modification this procedure results in formation two other known subnanoclusters, Au(22) Au(33). Whereas Au(23) are water soluble brightly fluorescent with quantum yields 2.5 1.3 %, respectively, Au(33) organic less fluorescent, yield 0.1 %. exhibits quenching...

Nowadays, through wall imaging (TWI) is an emerging topic of research in which one the most important tasks to minimize clutter detection accuracy can be improved. Clutter TWI due many reasons like coupling, antenna multiple re∞ections etc. To analyze reduction techniques, flrstly we indigenously assembled a system (i.e., step frequency continuous wave radar (SFCW)) UWB range (freq. 3.95GHz 5.85GHz), and difierent observations have been taken. We considered metallic plate more material with...

Water is an extensively self-associated liquid due to its extensive hydrogen bond (H-bond) forming ability. The resulting H-bonded network fluid exhibits nearly continuous absorption of light from the terahertz near-IR region. relatively weak bend+libration water combination band (centered at 2130 cm–1) has been largely overlooked as a reporter water's structure and dynamics despite location in convenient region IR for spectroscopic study. intermolecular nature leads unique signal that...

We synthesized fluorescent, porphyrin-anchored, Au22 clusters in a single step, starting from well-characterized Au25 protected with glutathione (−SG) by combined core reduction/ligand exchange protocol, at liquid−liquid interface. The prepared cluster was characterized UV/vis, photoluminescence, X-ray photoelectron spectroscopy (XPS), scanning electron microscopy, elemental analysis, and matrix-assisted laser desorption ionization mass spectrometry. absence of 672 nm intraband transition...

In photoinduced molecular reaction dynamics, the effects of electronic charge redistribution can lead to multiple pathways that are determined by nature initial structures involved and environment molecule interest is studied in. The β-diketones a common example this complexity. They show keto–enol tautomerism almost totally shifted toward enolic form. However, compared gas phase, photochemistry proceeds completely differently virtue solvent for these compounds, which used in commercial...

Polyethylene glycol (PEG) is a unique polymer material with enormous applicability in many industrial and scientific fields. Here, its use as macromolecular crowder to mimic the cellular environment vitro focus of present study. We show that femtosecond mid-IR pump–probe spectroscopy using three different IR probes, HDO, HN3, azido-derivatized crowder, provides complete stereoscopic information on water structure dynamics cytoplasm-like crowding environment. Our experimental results suggest...

Unit-cell volumes of four single-phase intermediate halite-sylvite solid solutions have been measured to pressures and temperatures ~28 kbar ~700 °C. Equation-of-state fitting the data yields thermal expansion compressibility as a function composition across chloride series. The variation product α0·K0 is linear (ideal) in between accepted values for halite sylvite. Taken separately, individual α0 K0 are not composition. shows maximum near consolute (XNaCl = 0.64) that exceeds value either...

We report the experimental determination of intramolecular enol-enol tautomerization rate an unsymmetric β-diketone, benzoylacetone, with femtosecond transient absorption in ultraviolet. Initially, there is equilibrium two possible enolic structures solution, which disturbed upon UV excitation by exciting a disproportionate fraction one form. Comparison to symmetric β-diketones, acetylacetone and dibenzoylmethane, suggests that ground-state proton transfer gives rise additional dynamics...

Recently, it was shown that the spectral graph theory is exceptionally useful for understanding not only morphological structural differences in ion aggregates but also similarities between an network and a water H-bonding highly concentrated salt solutions. Here, we present analysis results on osmolyte structures aqueous renal The quantitative analyses of adjacency matrices are graph-theoretical representations molecules H-bond provide ensemble average eigenvalue spectra degree...

In this contribution, we attempt to correlate the change in water dynamics a reverse micellar (RM) core caused by modification of interface mixing an anionic surfactant, sodium bis(2-ethylhexyl) sulfosuccinate (AOT), and nonionic tetraethylene glycol monododecyl ether (Brij-30), at different proportions, its consequent effect on reactivity water, measured monitoring solvolysis reaction benzoyl chloride (BzCl). The dimension RM droplets ratios AOT Brij-30 (XBrij-30) has been using dynamic...

Osmolytes are an integral part of living organism, e.g., the kidney uses sorbitol, trimethylglycine, taurine and myo-inositol to counter deleterious effects urea salt. Therefore, knowing that osmolytes' act either directly protein or mediated through water is great importance. Our experimental computational results show protecting osmolytes, trimethylglycine significantly modulate H-bonding network structure, although magnitude spatial extent osmolyte-induced perturbation greatly vary. In...

Fluorescent proteins undergoing green to red (G/R) photoconversion have proved be potential tools for investigating dynamic processes in living cells and photo-localization nanoscopy. However, the photochemical reaction during light induced G/R of fluorescent remains unclear. Here we report direct observation ultrafast time-resolved electron transfer (ET) photoexcitation EGFP mEos2 presence acceptor, p-benzoquinone (BQ). Our results show that excited state, neutral chromophore accepts...

We employ transient absorption from the deep-UV to visible region and fluorescence upconversion investigate photoinduced excited-state intramolecular proton-transfer dynamics in a biologically relevant drug molecule, 2-acetylindan-1,3-dione. The molecule is ß-diketone which electronic ground state exists as exocyclic enol with an H-bond. Upon excitation at 300 nm, first excited of initially populated, followed by ultrafast proton transfer (≈160 fs) form vibrationally hot endocyclic enol....