A5007484294- Lung Cancer Treatments and Mutations
- Head and Neck Cancer Studies
- Cancer Immunotherapy and Biomarkers
- Polyomavirus and related diseases
- Virus-based gene therapy research
- Prostate Cancer Treatment and Research
- Cancer Genomics and Diagnostics
- Cancer therapeutics and mechanisms
- Bacteriophages and microbial interactions
- Plant Virus Research Studies
- Brain Metastases and Treatment
- Effects of Radiation Exposure
- Metabolism and Genetic Disorders
- Immunotherapy and Immune Responses
- RNA and protein synthesis mechanisms
- Lung Cancer Research Studies
- Dysphagia Assessment and Management
- Metabolomics and Mass Spectrometry Studies
- Lung Cancer Diagnosis and Treatment
- interferon and immune responses
- Inflammatory Biomarkers in Disease Prognosis
- DNA and Nucleic Acid Chemistry
- Pharmacy and Medical Practices
- Acute Myeloid Leukemia Research
- Ethics in Clinical Research
University of California, San Diego
2011-2025
Moores Cancer Center
2023-2024
Memorial Sloan Kettering Cancer Center
1993-1994
Princeton University
1988
Weizmann Institute of Science
1985
Covalent attachment of myristic acid to pp60v-src, the transforming protein Rous sarcoma virus, was studied in a cell-free system. Using synthetic peptide containing first 11 amino acids mature pp60v-src polypeptide sequence as substrate, we probed lysates from variety cells and tissues for N-myristyl transferase (NMT) activity. Nearly every eucaryotic cell type tested contained NMT, including avian, mammalian, insect, plant cells. Since NMT activity detected rabbit reticulocyte lysates,...
Abstract Background The role of the inflammatory milieu in prostate cancer progression is not well understood. Differences signaling between localized and metastatic disease may point to opportunities for early intervention. Methods We modeled PCa by analyzing RNA-seq vs. patient samples, followed CIBERSORTx assess their immune cell populations. VHA CDW registry patients was analyzed anti-TNF clinical outcomes. Results observed statistically significant opposing patterns IL-6 TNFα expression...
257 Background: Immune surveillance in prostate cancer (PCa) relies on leukocyte trafficking into target tissues, a process mediated by endothelial adhesion molecules. This study investigates the role of E-selectin (SELE) PCa immune evasion and patient outcomes. Methods: We analyzed transcriptomic data from 7,523 samples (4,768 localized; 2,755 metastatic) molecularly profiled at Caris Life Sciences to assess SELE expression. Correlations between TNF molecule expression were examined normal...
Abstract Introduction: Head and neck squamous cell carcinoma (HNSCC) encompass a heterogenous classification of solid tumors that account for ∼5% the total new cancer cases globally. HNSCC presents as metastatic disease at estimated rates 4-25%, most commonly in bone, liver lung. Brain metastasis (BM) is uncommon particularly deadly. There need to better understand biomarkers may presage brain hopes identifying those patients requiring aggressive treatment head-off disease. Furthermore,...
Methylmalonate semialdehyde dehydrogenase (MMSDH) is a mitochondrial enzyme which can be acylated by myristoyl-CoA analogs (Deichaite, I., Berthiaume, L., Peseckis, S. M., Patton, W. F., and Resh, M. D. (1993) J. Biol. Chem. 268, 13788-13747). Here we describe the mechanisms mediate regulation of enzymatic activity bovine MMSDH long chain fatty acylation. The substrate specificity acylation reaction was measured in vitro using purified coenzyme A derivative an 125I-labeled acid...
Background Leukemia initiating cells (LIC) contribute to therapeutic resistance through acquisition of mutations in signaling pathways, such as NOTCH1, that promote self-renewal and survival within supportive niches. Activating NOTCH1 occur commonly T cell acute lymphoblastic leukemia (T-ALL) have been implicated resistance. However, the type context specific consequences activation, its role human LIC regeneration, sensitivity inhibition hematopoietic microenvironments had not elucidated....
The myristyl group makes a critical contribution to the processing, trafficking, and function of myristylated proteins. A series [omega-125I]iodo-fatty acids was synthesized in order elucidate group's membrane association pp60v-src, transforming protein Rous sarcoma virus. In vitro translation v-src mRNA employed monitor incorporation analogs into pp60v-src polypeptide. 12-Iodododecanoic, 13-iodotridecanoic, 14-iodotetradecanoic were selectively incorporated pp60v-src. One-dimensional...
Abstract Purpose Anti-PD-1 therapy provides clinical benefit in 40–50% of patients with relapsed and/or metastatic head and neck squamous cell carcinoma (RM-HNSCC). Selection anti- PD-1 is typically based on patient PD-L1 immunohistochemistry (IHC) which has low specificity for predicting disease control. Therefore, there a critical need biomarker that will predict to anti-PD-1 treatment high specificity. Methods Clinical outcomes data 103 RM-HNSCC were paired RNA-sequencing from...
Covalent attachment of myristic acid to pp60v-src, the transforming protein Rous sarcoma virus, was studied in a cell-free system. Using synthetic peptide containing first 11 amino acids mature pp60v-src polypeptide sequence as substrate, we probed lysates from variety cells and tissues for N-myristyl transferase (NMT) activity. Nearly every eucaryotic cell type tested contained NMT, including avian, mammalian, insect, plant cells. Since NMT activity detected rabbit reticulocyte lysates,...
Linear forms of simian virus 40 (SV40) DNA, when added to transfection mixtures containing circular SV40 and phi X174 RFI DNAs, enhanced the frequency SV40/phi recombination, as measured by infectious center in situ plaque hybridization monkey BSC-1 cells. The sequences required for enhancement recombination linear DNA reside within replication origin/regulatory region (nucleotides 5,171 5,243/0 128). Linearization did not increase frequency. recombinant structures arising from transfections...
Abstract Background Precision medicine incorporating genetic profiling is becoming a standard of care in medical oncology. However, the field radiation oncology there limited use and impact germline biomarkers on radiosensitivity, radioresistance, or patient outcomes after therapy poorly understood. In HNSCC, toxicity associated with treatment can cause delays early cessation which has been worse outcomes. Identifying potential help predict toxicity, as well response to treatment,...
Abstract Background Optimizing the therapeutic ratio for radiation therapy (RT) in head and neck squamous cell carcinoma (HNSCC) is uniquely challenging owing to high rates of early late toxicity involving nearby organs at risk. These toxicities have a profound impact on treatment compliance quality life. Emerging evidence suggests that RT dose alone cannot fully account variable severity RT-related adverse events (rtAEs) observed HNSCC patients. Next-generation sequencing has become an...
ABSTRACT Background Despite advances in cancer care and detection, more than 65% of patients with squamous cell the head neck (HNSCC) will develop recurrent and/or metastatic disease. The prognosis for these is poor a 5 year overall survival 39%. Recent treatment immunotherapy, including immune checkpoint inhibitors like pembrolizumab nivolumab, have resulted clinical benefit subset patients. There critical need to identify who from anti-PD-1 inhibitors. Methods Here we report findings...
Background Despite advances in cancer care and detection, >65% of patients with squamous cell the head neck (HNSCC) will develop recurrent and/or metastatic disease. The prognosis for these is poor a 5-year overall survival 39%. Recent treatment immunotherapy, including immune checkpoint inhibitors like pembrolizumab nivolumab, have resulted clinical benefit subset patients. There critical need to identify who from antiprogrammed death protein 1 (anti-PD-1) inhibitors. Methods Here, we...
We describe here the identification, purification, and characterization of a semialdehyde dehydrogenase with novel fatty acid binding function. The coenzyme A derivative an 125I-labeled long chain saturated (13-iodo-tridecanoate) was used to tag proteins which bind myristoyl-CoA. prominent 57 kDa band identified, isolated from bovine liver by high salt extraction followed ammonium sulfate precipitation. Sequential chromatographic separation using phenyl-Sepharose, hydroxyapatite,...
Linear forms of simian virus 40 (SV40) DNA, when added to transfection mixtures containing circular SV40 and phi X174 RFI DNAs, enhanced the frequency SV40/phi recombination, as measured by infectious center in situ plaque hybridization monkey BSC-1 cells. The sequences required for enhancement recombination linear DNA reside within replication origin/regulatory region (nucleotides 5,171 5,243/0 128). Linearization did not increase frequency. recombinant structures arising from transfections...
Abstract Purpose The purpose of this study was to evaluate if HPV status serves as an independent predictor early and late dysphagia outcomes when considered alongside standard patient characteristics dose metrics for head neck cancer patients treated with radiotherapy. Methods materials age, sex, smoking history, type (oropharyngeal vs non-oropharyngeal), status, were obtained 99 retrospective at our clinic Additionally each patient, the mean radiation pharynx, superior/middle/inferior...
A linear simian virus 40 origin-containing DNA fragment replicated in monkey COS cells, generating tandemly repeated (head-to-tail) structures. Electron microscopy revealed circle-and-tail configurations characteristic of rolling-circle replication intermediates. Circularization the same before transfection led to a theta type which generated supercoiled molecules.
6036 Background: BM is a rare complication of HNSCC that carries high rate morbidity and poor prognosis. Clinical risk factors, molecular characteristics, the immunogenicity are not well defined, leaving critical knowledge gap in this field. We performed one largest multi-institutional analyses summarizing clinical, molecular, immunologic profile 61 cases BM-HNSCC. Methods: conducted pooled analysis clinical characteristics pertaining to BM-HNSCC from 3 academic institutions (n=24)....
Brain metastasis (BM) is a rare but severe complication of head and neck squamous cell carcinoma (HNSCC), with limited knowledge molecular characteristics immunogenicity. We analyzed 61 cases HNSCC-BM from three academic institutions (n = 24) Foundation Medicine Inc (FMI, n 37). A subset underwent next-generation sequencing, multiple immunofluorescence, proximity ligation sequencing. Gene enrichment analysis compared alterations in FMI BM samples 37) local 4082). Demographics included:...
A linear simian virus 40 origin-containing DNA fragment replicated in monkey COS cells, generating tandemly repeated (head-to-tail) structures. Electron microscopy revealed circle-and-tail configurations characteristic of rolling-circle replication intermediates. Circularization the same before transfection led to a theta type which generated supercoiled molecules.
Determining the ownership of a patient's personal genomic data is important because it impacts how governed and shared, which has both clinical research implications for precision oncology. The 21st Century Cures Act enacted in December 2016 defined data, but governance research-grade remains hotly contested topic. many stakeholders often have competing perspectives about raw processed derived settings to weigh risks versus benefits sharing this with study participants. A growing number...
5022 Background: Advances in immunotherapy have had little impact prostate cancer (PCA). We previously examined the immune microenvironment both localized and metastatic PCA found that primary shows local inflammation/adaptive immunity whereas disease a shift towards suppression (Deichaite, 2022). Herein, we examine remodeling by evaluating changes gene expression between with heterogeneous treatment patterns samples. Methods: Tumors from (N = 5,419) were tested at Caris Life Sciences...