A5077285227- SARS-CoV-2 detection and testing
- Magnesium in Health and Disease
- SARS-CoV-2 and COVID-19 Research
- Parathyroid Disorders and Treatments
- Ion Transport and Channel Regulation
- Respiratory viral infections research
- Biosensors and Analytical Detection
- Viral gastroenteritis research and epidemiology
- Parasite Biology and Host Interactions
- Aquaculture disease management and microbiota
- Clostridium difficile and Clostridium perfringens research
- Hepatitis B Virus Studies
- Bacterial Identification and Susceptibility Testing
- Hepatitis C virus research
- Antibiotic Resistance in Bacteria
- Escherichia coli research studies
- Bird parasitology and diseases
- Diphtheria, Corynebacterium, and Tetanus
- Toxic Organic Pollutants Impact
- Effects and risks of endocrine disrupting chemicals
- COVID-19 diagnosis using AI
- COVID-19 Clinical Research Studies
- Mycobacterium research and diagnosis
- Influenza Virus Research Studies
- Electrolyte and hormonal disorders
St. Paul's Hospital
2008-2025
University of British Columbia
2015-2025
Providence Health Care
2013-2025
St. Paul's Hospital
2024
Mowi (Norway)
2020-2022
AIDS Vancouver
2021
British Columbia Children's Hospital
2019
Edinburgh Royal Infirmary
2015
York University
2014
Norwegian Biodiversity Information Centre
2002
False-negative severe acute respiratory syndrome coronavirus 2 test results can negatively impact the clinical and public health response to disease 2019 (COVID-19). We used droplet digital polymerase chain reaction (ddPCR) demonstrate that human DNA levels, a stable molecular marker of sampling quality, were significantly lower in samples from 40 confirmed or suspected COVID-19 cases yielded negative diagnostic (ie, false-negative results) compared with representative pool 87 specimens...
Abstract To screen all severe acute respiratory syndrome coronavirus 2–positive samples in Vancouver, British Columbia, Canada, and determine whether they represented variants of concern, we implemented a real-time reverse transcription PCR–based algorithm. We rapidly identified 77 with variants: 57 B.1.1.7, 7 B.1.351, an epidemiologic cluster 13 B.1.1.28/P.1.
We conducted patient chart reviews and whole-genome sequencing of wound specimens containing presumptive Corynebacterium ulcerans from Vancouver, British Columbia, Canada, during July 2019-July 2023. Sequencing confirmed 8/14 isolates were C. ramonii identified 2 distinct clusters. Molecular methods should be used to clinically differentiate potential toxin-producing spp.
ABSTRACT We performed phenotypic susceptibility testing and whole-genome sequencing to investigate in vitro mecillinam activity against multidrug-resistant (MDR) Shigella . Ninety-five isolates (53 [56%] sonnei 42 [44%] flexneri ) were included. The MIC 50 90 1 2 µg/mL, respectively, by agar dilution, all zone diameters ≥15 mm disc diffusion testing. No known clinically relevant resistance mutations (including cys B) detected. favorable profile absence of markers make pivmecillinam an agent...
Atlantic salmon (Salmo salar) were fed graded levels of dioxins (polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans) dioxin-like biphenyls (DLPCBs) in their diets for 7 months. The dioxin DLPCB concentrations both fillet whole body increased with increasing dietary exposure. DLPCBs transferred more efficiently from the feed to edible flesh than dioxins, contributed a higher proportion total toxic equivalents (TEQ). At end trial, maximum fish 1.9 2.3 pg WHO-TEQ g−1 fresh...
Infectious pancreatic necrosis virus (IPNV) is the causative agent of IPN, an important disease salmonids. IPNV infections result in either sub-clinical or overt and basis this difference not well understood. The objective present study was to determine VP2 gene associated with different forms clinical manifestation. Groups Atlantic salmon (Salmo salar L.) reared farms located IPN pressures were monitored from brood stock until grow-out over a 3 year period. Hatcheries A1 B1 as cooperating...
The ability of mobile stages Lepeophtheirus salmonis to transfer between salmon, Salmo salar L., hosts was examined by two methods. first these the removal certain on farmed fish, while second comprised placement lice clean fish in laboratory. In one‐sea‐winter 63% males and 52% female transferred new over a period 4 days. laboratory trials post‐smolts, 61% 69% 3 another experiment, free‐swimming adult male were added tank, 90% found after 24 h. Immigration trial cages large proportion...
Adult Atlantic salmon (Salmo salar) were fed on four diets containing polychlorinated dibenzo-p-dioxins (PCDDs), dibenzofurans (PCDFs), and biphenyls (PCBs) for 30 weeks. Lipid-normalized concentrations showed that all congeners equally partitioned between whole-fish fillet samples. Skinned accumulated approximately 30% of the total PCDD/F PCB content in fish. Accumulation efficiencies whole fish 43% 2,3,7,8-chlorinated dibenzofurans, 83% dioxin-like PCBs, 78% other congeners. Among PCDD/Fs,...
The consumer safety of farm-raised salmon could be improved by determining the transfer efficiency hazardous pollutants from fish feed to salmon. A controlled feeding trial for 30 weeks was carried out investigate polybrominated diphenyl ethers (PBDEs) in Atlantic (Salmo salar). Using three concentrations, an average 95% total PBDE content accumulated whole Skinned fillet 42–59% intake. Equal partitioning according lipid tissue demonstrated. formation less brominated PBDEs via preferential...
Abstract Nasopharyngeal swabs are critical to the diagnosis of respiratory infections including coronavirus disease 2019, but collection techniques vary. We compared 2 recommended nasopharyngeal swab in adult volunteers and found that rotation following contact did not recover additional nucleic acid (as measured by human DNA/RNA copy number). Rotation was also less tolerable for participants. Notably, both discomfort recovery were significantly higher Asian participants, consistent with...
Quantitative viral load assays have transformed our understanding of diseases. They hold similar potential to advance COVID-19 control and prevention, but SARS-CoV-2 tests are not yet widely available. molecular diagnostic tests, which typically employ real-time RT-PCR, yield semiquantitative results only. Droplet digital RT-PCR (RT-ddPCR) offers an attractive platform for RNA quantification. Eight primer/probe sets originally developed RT-PCR-based were evaluated use in RT-ddPCR; three...
Sanger sequencing or DNA hybridization have been the primary modalities for hepatitis B (HBV) resistance testing and genotyping; however, there are limitations, such as low sensitivity inability to detect novel mutations. Next-generation (NGS) HBV can overcome these but is limited guidance clinical microbiology laboratories validate this technology. In study, we describe an approach implementing deep pyrosequencing genotyping in a virology laboratory. A nested PCR targeting pol region of...
The clinical use of aminoglycosides often leads to renal magnesium wasting and hypomagnesemia. Of the nephron segments, both thick ascending limb Henle's loop distal tubule play significant roles in conservation but convoluted exerts final control urinary excretion. An immortalized mouse (MDCT) cell line has been extensively used study cellular mechanisms transport this segment. Peptide hormones, such as parathyroid hormone (PTH), glucagon, calcitonin, arginine vasopressin (AVP) stimulate...
The efficacy of teflubenzuron (Calicide®) for the treatment farmed Atlantic salmon (Salmo salar L.) infested with sea lice [Lepeophtheirus salmonis (Krøyer)] was investigated on two commercial farms. Teflubenzuron coated onto feed pellets administered orally at 10 mg per kg body weight day seven consecutive days. Fish were randomly sampled and numbers recorded from both treated control groups four sampling occasions post-medication. Statistically significant reductions in number L. fish...