A5079851122- Ultrasound and Hyperthermia Applications
- Nanoparticle-Based Drug Delivery
- Nanoplatforms for cancer theranostics
- Photoacoustic and Ultrasonic Imaging
- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- Radiomics and Machine Learning in Medical Imaging
- Ultrasound Imaging and Elastography
- Radiation Therapy and Dosimetry
- Advanced Drug Delivery Systems
- Cancer Research and Treatments
- RNA Interference and Gene Delivery
- Bacterial biofilms and quorum sensing
- Erythrocyte Function and Pathophysiology
- Bacteriophages and microbial interactions
- Cholinesterase and Neurodegenerative Diseases
- Virus-based gene therapy research
- Spectroscopy Techniques in Biomedical and Chemical Research
- Extracellular vesicles in disease
- Veterinary Oncology Research
- Blood properties and coagulation
- Lipid Membrane Structure and Behavior
- Orthopedic Infections and Treatments
- Salmonella and Campylobacter epidemiology
- Brain Tumor Detection and Classification
The University of Texas Southwestern Medical Center
2024
Southwestern Medical Center
2024
Oklahoma State University
2013-2023
Kansas State University
2023
Oklahoma State University Oklahoma City
2013-2021
Maulana Azad National Institute of Technology
2021
Motorola (United Kingdom)
2017
Creative Commons
2017
ESI - Post Graduate Institute of Medical Science and Research
2017
National Institutes of Health Clinical Center
2011-2016
Purpose: Objectives of this study were to: 1) develop iLTSL, a low temperature sensitive liposome co-loaded with an MRI contrast agent (ProHance® Gd-HP-DO3A) and doxorubicin, 2) characterise doxorubicin Gd-HP-DO3A release from iLTSL 3) investigate the ability magnetic resonance-guided high intensity focused ultrasound (MR-HIFU) to induce monitor content in phantoms vivo.Methods: was passively loaded actively doxorubicin. Doxorubicin quantified by fluorescence spectroscopic techniques,...
Purpose: Mild hyperthermia (40–45°C) is a proven adjuvant for radiotherapy and chemotherapy. Magnetic resonance guided high intensity focused ultrasound (MR-HIFU) can non-invasively heat solid tumours under image guidance. Low temperature-sensitive liposomes (LTSLs) release their drug cargo in response to (>40°C) may improve delivery when combined with mild hyperthermia. The objective of this study was develop implement clinically relevant MR-HIFU heating algorithm combination...
Using attenuated Salmonella that efficiently homes in solid tumors, here we developed thermobots actively transported membrane attached low-temperature sensitive liposome (LTSL) inside colon cancer cells for triggered doxorubicin release and simultaneous polarized macrophages to M1 phenotype with high intensity focused ultrasound (HIFU) heating (40-42 °C). Biocompatibility studies showed the synthesized were highly efficient LTSL loading without impacting its viability. Thermobots...
Rationale: Some studies have shown that the local activation of immunogenic cell death (ICD) by upregulating calreticulin (CRT) expression in solid tumors can improve antitumor effects.Although a promising approach, key current challenge ICD tumor therapy is absence clinically translatable method for reproducibly inducing CRT expression.Herein, we report novel calreticulin-nanoparticle (CRT-NP) enhances and synergizes with focused ultrasound (FUS) to achieve systemic effects.Methods:...
Treatment and eradication of intracellular pathogens such as Brucella is difficult because infections are localized within phagocytic cells most antibiotics, although highly active in vitro, do not actively pass through cellular membranes. Thus, an optimum strategy to treat these should address targeting drugs the compartment where bacteria replicate, prolong release antibiotics so that number doses associated toxicity can be reduced. We incorporated streptomycin doxycycline into...
We investigated the capability of biodegradable silica xerogel as a novel carrier antibiotic and efficacy treatment compared to that with same dose free drug against murine salmonellosis. The molecules (31%) entrapped in sol-gel matrix remained biologically active form, bactericidal effect was retained upon release. vitro release profiles gentamicin from xerogel-polyethylene glycol (PEG) were distinctly different at pH 7.4. A delayed observed network (57% 33 h), addition 2% PEG, rate reached...
Background: Advanced stage cancers with a suppressive tumor microenvironment (TME) are often refractory to immune checkpoint inhibitor (ICI) therapy. Recent studies have shown that focused ultrasound (FUS) TME-modulation can synergize ICI therapy, but enhancing survival outcomes in poorly immunogenic tumors remains challenging. Here, we investigated the role of based boiling histotripsy (HT) and in-situ anti-CD40 agonist antibody (αCD40) combinatorial therapy therapeutic efficacy against...
The success of melanoma immunotherapy is dependent on the presence activated and functional T-cells in tumors. objective this study was to investigate impact local-focused ultrasound (FUS) heating (∼42–45 °C) in-situ anti-CD-40 agonistic antibody enhancing T-cell function for immunotherapy. We compared following groups mice with bilateral flank B16 F10 melanoma: (1) Control, (2) FUS, (3) CD-40, (4) CD-40 + FUS (FUS40). applied ∼15 min right tumor, intratumoral injections were performed...
Introduction Anti-CD40 agonistic antibody (αCD40), an activator of dendritic cells (DC) can enhance antigen presentation and activate cytotoxic T-cells against poorly immunogenic tumors. However, cancer immunotherapy trials also suggest that αCD40 is only moderately effective in patients, falling short achieving clinical success. Identifying factors decrease immune-stimulating effects aid the translation this agent to reality. Method/Results Here, we reveal β-adrenergic signaling on DCs...
Multicellular Tumor Spheroids (MCTS) strongly resemble tumor tissues, which makes them useful tools for radiation biology studies and screening of various chemotherapeutics.The goal this pilot study was to use MCTS as an in vitro model determine the response cells low temperature-sensitive liposomes (LTSLs) encapsulating doxorubicin (Dox) proton beam radiotherapy (PBRT).Prior treatment, were characterized morphology LTSLs size, encapsulation efficiency, ability thermally release Dox (a...
Chronic non-healing wound infections require long duration antibiotic therapy, and are associated with significant morbidity health-care costs. Novel approaches for efficient, readily-translatable targeted localised antimicrobial delivery needed. The objectives of this study were to 1) develop low temperature-sensitive liposomes (LTSLs) containing an agent (ciprofloxacin) induced release at mild hyperthermia (∼42 °C), 2) characterise in vitro ciprofloxacin release, efficacy against...
Purpose: Localised hyperthermia in rodent studies is challenging due to the small target size. This study describes development and characterisation of an MRI-compatible high-intensity focused ultrasound (HIFU) system perform localised mild treatments models. Material methods: The platform consisted animal HIFU system, transducers with sector-vortex lenses, a custom-made receive coil, means maintain systemic temperatures rodents. was integrated into 3T MR imager. Control software developed...
Pluronic based core-shell nanostructures encapsulating gentamicin were designed in this study. Block copolymers of (PAA(+/-)Na-b-(PEO-b-PPO-b-PEO)-b-PAA(+/-)Na) blended with PAA(-) Na(+) and complexed the polycationic antibiotic to form nanostructures. Synthesized had a hydrodynamic diameter 210 nm, zeta potentials -0.7 (+/-0.2), incorporated approximately 20% by weight gentamicin. Nanostructures upon co-incubation J774A.1 macrophage cells showed no adverse toxicity vitro. administered vivo...
Novel approaches allowing efficient, readily translatable image-guided drug delivery (IGDD) against solid tumours is needed. The objectives of this study were to: 1) develop echogenic low temperature sensitive liposomes (E-LTSLs) loaded with an ultrasound (US) contrast agent (perfluoropentane, PFP), 2) determine the in vitro and vivo stability encapsulation, 3) co-encapsulate characterise doxorubicin (Dox) E-LTSL, cellular uptake cytotoxicity combination high intensity focused (HIFU).E-LTSLs...