A5063944758- Ocular Infections and Treatments
- Ocular Diseases and Behçet’s Syndrome
- Antifungal resistance and susceptibility
- Intraocular Surgery and Lenses
- Ocular Surface and Contact Lens
- Retinal and Macular Surgery
- Bacterial biofilms and quorum sensing
- Facial Rejuvenation and Surgery Techniques
- Glaucoma and retinal disorders
- Bacterial Identification and Susceptibility Testing
- Herpesvirus Infections and Treatments
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Vibrio bacteria research studies
- Yersinia bacterium, plague, ectoparasites research
- Proteoglycans and glycosaminoglycans research
- Antibiotic Resistance in Bacteria
- Inflammasome and immune disorders
Manipal Academy of Higher Education
2021-2024
L V Prasad Eye Institute
2021-2024
Purpose: Heparanase (HPSE) cleaves heparan sulfate proteoglycans during herpes simplex virus-1 (HSV-1) infection, aiding in viral egress and disease progression. Its action has been well established vitro vivo models, but its relevance human patients remains unclear. This study aimed to specifically evaluate tear HPSE levels of with keratitis (HSK) correlate these findings a commonly used murine model. Methods: Tear samples from patient mice were collected at LV Prasad Eye Institute,...
Purpose: Neutrophils are known mediators of innate immunity, yet their effector function in herpesvirus infections remains poorly understood. Here, we elucidate the mechanistic action and pivotal role neutrophil extracellular traps (NETs) during herpes simplex virus type 1 (HSV-1) ocular infection. Methods: were collected from mice for HSV-1 infection, fluorescence imaging, immunoblotting assay. Tear samples healthy subjects patients with at L. V. Prasad Eye Institute, India, University...
Abstract Background Global concerns have been raised due to upward trend of Multi-drug Resistant (MDR) Pseudomonas aeruginosa reports in ocular infections. Our aim was characterize the virulence determinants MDR P. causing Methods strains were isolated from 46 patients with conjunctivitis (2), endophthalmitis (11) and active keratitis (25) seen at our Institute, between 2016 2020. The isolates identified by Vitek-2 characterized based on growth kinetics, biofilm formation, motility,...
To evaluate vitreous Galactomannan(GM) and 1,3 β-D-Glucan (BDG) levels in the diagnosis of fungal endophthalmitis, with emphasis on culture-negative cases.Vitreous from 31 clinically suspected endophthalmitis patients 11 controls were evaluated for GM BDG using ELISA Kits. The Receiver Operating Characteristic (ROC) curves diagnostic significance was calculated.The median culture-positive (60.83pg/ml) (59.9pg/ml) samples higher than (51.2pg/ml) control group. (1.47pg/ml) (1.52pg/ml) also...
Purpose: To determine if retrobulbar injection of hyaluronidase reaches the vitreous cavity, and to its concentration in vitreous. Methods: Prospective case-control study. Patients undergoing evisceration with implant for noninfective blind eyes were enrolled Before evisceration, a 3,000 IU (2 ml) was injected. Time from vivo sampling posterior noted. Vitreous samples controls obtained patients vitrectomy retinal detachment or diabetic retinopathy. Concentration assessed all 30 samples. An...