Photodynamic therapy (PDT), which relies on photosensitizers (PS) and light to generate reactive oxygen species kill cancer cells or bacteria, has attracted much attention in recent years. PSs with both bright emission efficient singlet generation have also been used for image-guided PDT. However, simultaneously achieving effective 1 O2 generation, long wavelength absorption, stable near-infrared (NIR) low dark toxicity a single PS remains challenging. In addition, it is well known that when...

Bacterial infection is one of the most serious physiological conditions threatening human health. There an increasing demand for more effective bacterial diagnosis and treatment through noninvasive theranostic approaches. Herein, a new strategy reported to achieve in vivo metabolic labeling bacteria use MIL-100 (Fe) nanoparticles (NPs) as nanocarrier precise delivery 3-azido-d-alanine (d-AzAla). After intravenous injection, NPs can accumulate preferentially degrade rapidly within high H2 O2...

Abstract Ferroptosis regulates cell death through reactive oxygen species (ROS)‐associated lipid peroxide accumulation, which is expected to affect the structure and polarity of droplets (LDs), but with no clear evidence. Herein, we report first example an LD/nucleus dual‐targeted ratiometric fluorescent probe, CQPP , for monitoring changes in cellular microenvironment. Due donor–acceptor it offers fluorescence emission lifetime signals that reflect variations. Using nucleus imaging as a...

An activatable fluorescent nanoprobe with aggregation-induced emission signature is developed. The nonfluorescent, but can be induced to emit intensely after reaction peroxynitrite forming an intramolecular hydrogen bond. Excellent performance for selective in vivo imaging of inflammation elevated generation and efficient visualization treatment efficacy anti-inflammatory agents demonstrated.

Abstract Bio‐orthogonal tumor labeling is more effective in delivering imaging agents or drugs to a site than active targeting strategy owing covalent ligation. However, date, tumor‐specific through bio‐orthogonal largely relies on body clearance differentiate target from the intrinsic probe signal lack of light‐up probes for vivo labeling. Now first based fluorogen with aggregation‐induced emission fluorescence turn‐on presented. The has low background aqueous media, showing negligible...

The nucleus is considered the ideal target for anti-tumor therapy because DNA and some enzymes in are main causes of cell canceration malignant proliferation. However, nuclear drugs with good biosafety high efficiency cancer treatment rare. Herein, a nuclear-targeted material MeTPAE aggregation-induced emission (AIE) characteristics was developed based on triphenylamine structure skeleton. can not only interact histone deacetylases (HDACs) to inhibit proliferation but also damage telomere...

Phototherapy, using light as a therapeutic modality, has been widely applied in treating various diseases, especially cancer. This approach is valued for its non-invasive nature and minimal risk of drug resistance. However, phototherapy faces challenges such the undesired delivery phototherapeutic agents, potential phototoxicity, limited penetration into tissues. Lanthanide-doped nanoparticles offer attractive opportunities to improve efficacy photodynamic therapy. These are distinguished by...

A new bottom-up nanocrystallization method is developed to fabricate highly fluorescent organic nanocrystals in aqueous media using an aggregation-induced emission fluorogen (AIEgen) as example. The strategy leads the fabrication of uniform 110 ± 10 nm size media, which shows over 400% increase brightness compared amorphous nanoaggregates.

Monitoring and understanding long-term fate regenerative therapy of administrated stem cells in vivo is great importance. Herein we report organic nanodots with aggregation-induced emission characteristics (AIE dots) for tracking adipose-derived (ADSCs) their capacity living mice. The AIE dots possess high fluorescence (with a quantum yield 25 ± 1%), excellent biological photophysical stabilities, low toxicity, superb retention ADSCs negligible interference on pluripotency secretome. These...

A series of D–A′–π–A type photosensitizers were designed and synthesized to show strong aggregation-induced far red near infrared emission very effective ¹O₂ generation simultaneously, have been successfully used for image-guided photodynamic anticancer therapy.

Abstract Protected by the host cells, hidden intracellular bacteria are typically difficult to kill common antibiotics and cannot be visualized without complex cellular pretreatments. Herein, we successfully developed a bacteria‐metabolizable dual‐functional probe TPEPy‐ d ‐Ala, which is based on ‐alanine photosensitizer with aggregation‐induced emission for fluorescence turn‐on imaging of in living cells photodynamic ablation situ. Once metabolically incorporated into bacterial...

Integration of AIEgens with upconversion nanoparticles inhibits tumor recurrence and metastasis.

Abstract There has been a surging interest in the synthesis of activatable photosensitizers (PSs) as they can be selectively activated with minimum nonspecific phototoxic damages for photodynamic therapy (PDT). Conventional strategies to realize PSs are only applicable limited number molecules. Herein, simple and general strategy yield by coupling MIL‐100 (Fe) (MIL: Materials Institute Lavoisier) different kinds is presented. Specifically, when encapsulated into (Fe), photosensitization...

Abstract Liposomes have been used as popular drug delivery systems for cancer therapy. However, it is difficult to track traditional liposome in an efficient and stable fashion assess their efficacy biodistribution after administration. Meanwhile, conventional fluorescent liposomes containing optical tracers face the challenge of aggregation‐caused quenching. Herein, we report a strategy integration aggregation‐induced emission fluorogen with yield AIEgen–lipid conjugate, termed “AIEsome”....

Abstract We demonstrate that the incorporation of one or two amino acids phenylalanine (F) 4‐fluoro ( f F) will greatly lower background fluorescence intensities conventional quenched probes with quenchers. This enhanced quenching effect was due to synergetic aggregation caused and presence a quencher. Such strategy not affect enzyme recognition properties probes. also demonstrated our self‐assembled nanoprobe showed better performance in cells for detection cell apoptosis than unassembled...

Abstract Owing to efficient singlet oxygen ( 1 O 2 ) generation in aggregate state, photosensitizers (PSs) with aggregation‐induced emission (AIE) have attracted much research interests photodynamic therapy (PDT). In addition high efficiency, strong molar absorption long‐wavelength range and near‐infrared (NIR) are also highly desirable, but difficult achieve for AIE PSs since the twisted structures moieties usually lead short‐wavelength range. this contribution, through acceptor...

Direct monitoring of multiple enzyme activities in a given biological process is extremely important for disease diagnosis.

Abstract The complex tumor microenvironment (TME) and nonspecific drug targeting limit the clinical efficacy of photodynamic therapy in combination with chemotherapy. Herein, a metal–organic framework (MOF) assisted strategy is reported that modulates TME by reducing hypoxia intracellular glutathione (GSH) offers targeted delivery controlled release trapped chemodrug. Platinum(IV)‐diazido (Pt(IV)) loaded inside Cu(II) carboxylate‐based MOF, MOF‐199, an aggregation‐induced‐emission...

Bioactive peptides derived from proteins generally need to be folded into secondary structures activate downstream signaling pathways. However, synthetic typically form random-coils, thus losing their bioactivities. Here, we show that by introducing a self-assembling peptide motif and using different preparation pathways, insulin-like growth factor-I (IGF-1) can an α-helix β-sheet. The β-sheet one exhibits low dissociation constant the IGF-1 receptor (IGF-1R, 11.5 nM), which is only about 3...

Traditional antibacterial procedures are getting inefficient due to the emergence of antimicrobial resistance, which makes alternative treatments in urgent demand. However, selectivity toward infectious bacteria is still challenging. Herein, by taking advantage self-directed capture macrophages, we developed a strategy realize precise vivo photodynamic therapy (APDT) through adoptive photosensitizer-loaded macrophage transfer. TTD with strong reactive oxygen species (ROS) production and...

Abstract The development of Type I photosensitizers (PSs) is great importance due to the inherent hypoxic intolerance photodynamic therapy (PDT) in microenvironment. Compared II PSs, PSs are less reported absence a general molecular design strategy. Herein, we report that combination typical PS and natural substrate carvacrol (CA) can significantly facilitate pathway efficiently generate superoxide radical (O 2 –• ). Detailed mechanism study suggests CA activated into thymoquinone (TQ) by...