A5058422116- RNA modifications and cancer
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- Congenital heart defects research
- CRISPR and Genetic Engineering
- Protease and Inhibitor Mechanisms
- Pluripotent Stem Cells Research
- Tissue Engineering and Regenerative Medicine
- Piperaceae Chemical and Biological Studies
- Polysaccharides and Plant Cell Walls
- Microbial Metabolites in Food Biotechnology
- RNA Interference and Gene Delivery
- Biofuel production and bioconversion
- Genetics and Neurodevelopmental Disorders
- Traditional and Medicinal Uses of Annonaceae
- Ginger and Zingiberaceae research
- Epigenetics and DNA Methylation
- Molecular Biology Techniques and Applications
- Bioactive Compounds and Antitumor Agents
- Biological Research and Disease Studies
- Heavy Metals in Plants
- Mesenchymal stem cell research
- Breast Cancer Treatment Studies
- S100 Proteins and Annexins
- Bone and Dental Protein Studies
Fundação Oswaldo Cruz
2017-2025
Fundação Carlos Chagas
2018-2025
University of Colorado Boulder
2023-2024
Universidade Federal do Paraná
2011-2017
Piper aleyreanum is a small tree that widely distributed in tropical and subtropical regions, mostly North South America, used as an immunomodulator, analgesic antidepressant folk medicine.This study was designed to investigate the antinociceptive, anti-inflammatory gastric antiulcer activities of essential oils from aerial parts (EOPa) rodents.The antinociceptive effects orally administered EOPa were evaluated mice subjected formalin pleurisy models, respectively. We also pretreated rats...
Cell differentiation during development is orchestrated by precisely coordinated gene expression programs. While some regulatory mechanisms are well understood, there a significant room to explore unresolved aspects of lineage choice and cell-fate decisions, as many events in these processes still not fully elucidated. Given that, influenced only transcriptional control but also post-transcriptional events. Here, we described the presence regulation on commitment across all three embryonic...
Numerous studies have now demonstrated that lncRNAs can influence gene expression programs leading to cell and organismal phenotypes. Typically, lncRNA perturbations concomitant changes in are measured on the timescale of many hours days. Thus, we currently lack a temporally grounded understanding primary, secondary, tertiary relationships lncRNA-mediated transcriptional epigenetic regulation—a prerequisite elucidating mechanisms. To begin address when where regulates expression, genetically...
Abstract The adipogenic process is characterized by the expression of adipocyte differentiation markers that lead to changes in cell metabolism and accumulation lipid droplets. Moreover, during early adipogenesis, cells undergo a strong downregulation translational activity with decrease size, proliferation migration. In present study, we identified after 24 hours induction, human adipose tissue-derived stem (hASCs) G1-cell cycle arrest consistent reduced proliferation, this effect was...
Tabebuia avellanedae (syn. Handroanthus impetiginosus ) is popularly known as ‘ipê‐roxo’ and has been used in folk medicine anti‐inflammatory the treatment of ulcers, bacterial fungal infections. This study evaluated gastric ulcer healing property ethanolic extract (EET) barks from investigated mechanisms that may underlie this effect. Rats were treated with EET (twice a day for 7 days) after induction chronic ulcers by 80% acetic acid. Following treatment, histological immunohistochemical...
Abstract Breast cancer is a heterogeneous disease with differences in its clinical, molecular and biological features. Traditionally, immunohistochemical markers together clinicopathologic parameters are used to classify breast predict outcome. Triple-negative (TNBC) particular type of that defined by lack expression hormonal receptors the HER2 gene. Most cases TNBC also have basal-like phenotype (BLBC) cytokeratin 5/6 and/or EGFR. A basal marker alone insufficient for better understanding...
Metastasis accounts for more than 90% of cancer deaths. Cells from primary solid tumors may invade adjacent tissues and migrate to distant sites where they establish new colonies. The tumor microenvironment is now recognized as an important participant in the signaling that induces cell migration. An essential process metastasis extracellular matrix (ECM) degradation by metalloproteases (MMPs), which allows cells local reach blood vessels. members this protein family include gelatinase A, or...
Mesenchymal stem cells (MSCs) have been widely studied with regard to their potential use in cell therapy protocols and regenerative medicine. However, a better comprehension about the factors molecular mechanisms driving differentiation is now mandatory improve our chance manipulate MSC behavior benefit future applications. In this work, we aimed study gene regulatory networks at an early step of osteogenic differentiation. Therefore, analyzed both total mRNA fraction associated polysomes...
Cardiac cell fate specification occurs through progressive steps, and its gene expression regulation features are still being defined. There has been an increasing interest in understanding the coordination between transcription post-transcriptional during differentiation processes. Here, we took advantage of polysome profiling technique to isolate high-throughput sequence ribosome-free polysome-bound RNAs cardiomyogenesis.We showed that exhibit cardiomyogenic commitment mesoderm-to-cardiac...
After industrial processing, one-third of sugarcane culms is converted into residual bagasse. The xylan-rich hemicellulose components the bagasse were extracted with hot aqueous alkali (AX-CRUDE). Approximately 82% hemicelluloses was precipitated ethanol (AX-PET). Both AX-CRUDE and AX-PET contained an arabinoxylan as confirmed by 13C NMR methylation analysis. Fraction fed to female Wistar rats ethanol-induced gastric lesions. Oral administrations 30, 100, 300 mg/kg reduced lesion area over...
Abstract The regulation of gene expression acts at numerous complementary levels to control and refine protein abundance. analysis mRNAs associated with polysomes, called polysome profiling, has been used investigate the post-transcriptional mechanisms that are involved in different biological processes. Pluripotent stem cells able differentiate into a variety cell lineages, commitment progression is carefully orchestrated. Genome-wide profiling provided possibility transcriptional changes...
Human pluripotent stem cells are an important tool for the study of developmental processes, such as cardiomyogenic differentiation. Despite advances made in this field, molecular and cellular signals involved commitment embryonic to cardiac phenotype still under investigation. Therefore, focuses on identifying extracellular vitro differentiation human cells. Using a three-dimensional protocol, conditioned medium matrix embryoid body cultures were collected characterized at four specific...
Successful heart development depends on the careful orchestration of a network transcription factors and signaling pathways. In recent years, in vitro cardiac differentiation using human pluripotent stem cells (hPSCs) has been used to uncover intricate gene-network regulation involved proper formation function heart. Here, we searched for uncharacterized cardiac-development genes by combining temporal evaluation specification with an analysis gene expression fetal adult tissue. We discovered...
Summary Introduction: Breast cancer is the most cause of death, and approximately 90% these deaths are due to metastases. Matrix metalloproteinase-2 (MMP-2) gelatinase activity able degrade a major constituent tumor microenvironment, type IV collagen. Two well-established proteins used as markers in clinical practice for breast receptors estrogen (ER) progesterone (PR). Although presence has been associated with better prognosis, loss can occur during progression, subsequent resistance...
Understanding the cell differentiation process involves characterization of signaling and regulatory pathways. The coordinated action involved in multilevel regulation determines commitment stem cells their into a specific lineage. Cellular metabolism plays relevant role modulating expression genes, which act as sensors extra-and intracellular environment. In this work, we analyzed mRNAs associated with polysomes by focusing on profile metabolism-related genes during cardiac human embryonic...
Abstract Numerous studies have now demonstrated that lncRNAs can influence gene expression programs leading to cell and organismal phenotypes. Typically, lncRNA perturbations concomitant changes in are measured on the timescale of many hours days. Thus, we currently lack a temporally grounded understanding primary, secondary, tertiary relationships lncRNA-mediated transcriptional epigenetic regulation – despite being prerequisite elucidating mechanisms. To begin address when where regulates...
<title>Abstract</title> <bold>Background:</bold> Cell differentiation during development is orchestrated by precisely coordinated gene expression programs. While mechanisms such as the maintenance of pluripotent states are well-understood, others like lineage choice and cell-fate decisions remain poorly comprehended. Given that influenced not only transcriptional control but also post-transcriptional events, we employed monolayer protocols to delineate early events in human embryonic stem...
Long non-coding RNAs (lncRNAs) have been found to be involved in many biological processes, including the regulation of cell differentiation, but a complete characterization lncRNA is still lacking. Additionally, there evidence that lncRNAs interact with ribosomes, raising questions about their functions cells. Here, we used developmentally staged protocol induce cardiogenic commitment hESCs and then investigated differential association polysomes. Our results identified both ribosome-free...