A5002759004- RNA Research and Splicing
- SARS-CoV-2 detection and testing
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- COVID-19 epidemiological studies
- Genetics, Aging, and Longevity in Model Organisms
- Cardiomyopathy and Myosin Studies
- Neuroscience and Neuropharmacology Research
- Cancer-related molecular mechanisms research
- SARS-CoV-2 and COVID-19 Research
- T-cell and B-cell Immunology
- MicroRNA in disease regulation
- Ion channel regulation and function
- Circular RNAs in diseases
- CAR-T cell therapy research
- Cancer-related gene regulation
- Lymphatic System and Diseases
- RNA Interference and Gene Delivery
- Molecular Biology Techniques and Applications
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Neurogenetic and Muscular Disorders Research
- Extracellular vesicles in disease
- Genetics and Neurodevelopmental Disorders
University of Colorado Anschutz Medical Campus
2010-2025
University of Colorado Boulder
2007-2024
Howard Hughes Medical Institute
2014-2016
University of Colorado Denver
2007-2011
Duke University
2002-2008
Duke University Hospital
2004-2008
Duke Medical Center
2003-2008
Fitzsimons Army Medical Center
2006
Backspliced circular RNAs (circRNAs) are prevalent in many eukaryotic systems and spliced from thousands of different genes. Where examined, circRNAs often highly stable the mechanisms by which cells degrade and/or clear unknown. Here we investigated possibility that can eliminate into extracellular space, possibly within released vesicles such as exosomes microvesicles. From three cell lines examining multiple circRNAs, show vesicle (EVs) preparations recovered culture conditioned media...
We analyze data from the fall 2020 pandemic response efforts at University of Colorado Boulder, where more than 72,500 saliva samples were tested for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) using qRT-PCR. All collected individuals who reported no symptoms associated with COVID-19 on day collection. From these, 1,405 positive cases identified. The distribution viral loads within these asymptomatic was indistinguishable what has been previously observed in symptomatic...
The Ca(2+)/calmodulin (CaM)-dependent protein kinase II (CaMKII)beta has morphogenic functions in neurons not shared by the alpha isoform. CaMKIIbeta contains three exons (v1, v3, and v4) present CaMKIIalpha gene, two of these (v1 are subject to differential alternative splicing. We show here that CaMKIIbeta, but alpha, mediated bundling F-actin filaments vitro. Most importantly, inclusion exon v1 was required for association with cytoskeleton within cells. CaMKIIbetae, which is dominant...
Here, we develop a simple molecular test for SARS-CoV-2 in saliva based on reverse transcription loop-mediated isothermal amplification. The has two steps: (1) heat with stabilization solution and (2) detect virus by incubating primer/enzyme mix. After incubation, samples containing the genome turn bright yellow. Because this is pH dependent, it can react falsely to some naturally acidic samples. We report unique protocols that rendered 295 healthy compatible test, producing zero false...
To assist in the COVID-19 public health guidance on a college campus, daily composite wastewater samples were withdrawn at 20 manhole locations across University of Colorado Boulder campus. Low-cost autosamplers fabricated in-house to enable an economical approach this distributed study. These sample stations operated from August 25th until November 23rd during fall 2020 semester, with 1512 collected. The concentration SARS-CoV-2 each was quantified through two comparative reverse...
Abstract Whereas it is known that voltage‐gated calcium channels play important roles during development, potential embryonic of sodium have received much less attention. Voltage‐gated consist pore‐forming α‐subunits (Na v 1) and auxiliary β‐subunits. Here, we report the larval expression patterns for all eight members gene family ( scna ) coding zebrafish Na 1 proteins. We find each displays a distinct pattern temporally spatially dynamic stages. Overall, our findings indicate occurs...
Four CaMKII isoforms are encoded by distinct genes, and alternative splicing within the variable linker-region generates additional diversity. The α β largely brain-specific, where they mediate synaptic functions underlying learning, memory cognition. Here, we determined splice-variant distribution among different mouse brain regions. Surprisingly, nuclear variant αB was detected in all regions, even dominated hypothalamus stem. For CaMKIIβ, full-length most regions (with higher amounts of...
The coronavirus disease 2019 pandemic spread to >200 countries in <6 months. To understand spread, determining transmission rate and defining factors that increase risk are essential. Most cases asymptomatic, but people with asymptomatic infection have viral loads indistinguishable from those symptomatic people, they do transmit severe acute respiratory syndrome 2 (SARS-CoV-2). However, often undetected. Given high residence hall student density, the University of Colorado Boulder...
The lymphatic system plays a central role in lipid absorption, which transports chylomicrons from the small intestine to circulation. However, molecular mechanism by get into intestinal lymphatics is unknown. Here we demonstrated that GPR182, receptor endothelial cells (LECs), mediates dietary fat absorption. GPR182 knockout mice are resistant dietary-induced obesity. ablation leads poor absorption and thereby delay growth during development. binds endocytoses lipoproteins broadly....
Alternative splicing of fibroblast growth factor receptor 2 (FGFR2) occurs in a cell-type-specific manner with the mutually exclusive use exon IIIb or IIIc. Specific inclusion is observed epithelial cells, whereas IIIc seen mesenchymal cells. Epithelium-specific activation and repression are coordinately regulated by intronic activating sequence (IAS2) activator repressor (ISAR) elements FGFR2 pre-mRNA. Previously, it has been suggested that IAS2 20-nucleotide core ISAR form stem structure...
Numerous studies have now demonstrated that lncRNAs can influence gene expression programs leading to cell and organismal phenotypes. Typically, lncRNA perturbations concomitant changes in are measured on the timescale of many hours days. Thus, we currently lack a temporally grounded understanding primary, secondary, tertiary relationships lncRNA-mediated transcriptional epigenetic regulation—a prerequisite elucidating mechanisms. To begin address when where regulates expression, genetically...
In many Caenorhabditis elegans pre-mRNAs, the RNA sequence between 5' cap and first 3' splice site is replaced by trans-splicing a short spliced leader (SL) from Sm snRNP, SL1. C. also utilizes similar SL2, to trans-splice at sites genes in polycistronic pre-mRNAs operons. How do SL1 SL2 snRNPs function different contexts? Here we show that snRNP contains complex of SL75p SL21p, which are homologs novel proteins previously reported Ascaris SL snRNP. Interestingly, does not contain these...
Abstract Here, we develop a simple molecular test for SARS-CoV-2 in saliva based on reverse transcription loop-mediated isothermal amplification (RT-LAMP). The has two steps: 1) heat with stabilization solution, and 2) detect virus by incubating primer/enzyme mix. After incubation, samples containing the genome turn bright yellow. Because this is pH dependent, it can react falsely to some naturally acidic samples. We report unique protocols that rendered 295 healthy compatible test,...
Alternative splicing of mammalian transcripts, which yields many diverse protein products from one gene, is the rule and not exception. Although mechanisms that govern alternative are being unraveled, little known about evolution this critical engine proteome diversity. Here we present a phylogenetic analysis sea urchin to humans unit encoding third Ig domain fibroblast growth factor receptors. The remarkable conservation intronic control elements, both in structure function, indicates...
T-bet and FOXO1 are transcription factors canonically associated with effector memory T cell fates, respectively. During an infectious response, these direct the development of CD8
Abstract Many of the biological functions performed by RNA are mediated RNA-binding proteins (RBPs), and understanding molecular basis these interactions is fundamental to biology. Here, we present massively parallel assay combined with immunoprecipitation (MPRNA-IP) for in vivo high-throughput dissection RNA–protein describe statistical models identifying domains parsing structural contributions RNA. By using custom pools tens thousands sequences containing systematically designed...
Protection from pathogens relies on both humoral (antibody-mediated) and cellular (T cell-mediated) responses. While infections robustly elicit types of immunity, currently approved vaccine adjuvants largely fail to induce T cell responses par with that instigated by infections. Our goal was investigate the transcriptional programming supports formation CD8+ cells elicited subunit vaccines compared those data show vaccine-elicited represent a transcriptionally unique subset activated high...
ABSTRACT In 2019-2020, the COVID-19 pandemic spread to over 200 countries in less than six months. To understand basis of this aggressive spread, it is essential determine transmission rate and define factors that increase risk transmission. One complication large fraction asymptomatic cases, particularly young populations: these individuals have viral loads indistinguishable from symptomatic people do transmit SARS-CoV-2 virus, but they often go undetected. As university students living...
Spliced leader (SL) trans-splicing in Caenorhabditis elegans attaches a 22-nucleotide (nt) exon onto the 5' end of many mRNAs. A particular class SL, SL2, splices mRNAs downstream operon genes. Here we use an embryonic extract-based vitro splicing system to show that SL2 specificity information is encoded within polycistronic pre-mRNA, and recapitulated vitro. We define RNA sequence required for trans-splicing, U-rich (Ur) element, through mutational analysis bioinformatics as short...
Abstract We analyze data from the Fall 2020 pandemic response efforts at University of Colorado Boulder (USA), where more than 72,500 saliva samples were tested for SARS-CoV-2 using quantitative RT-PCR. All collected individuals who reported no symptoms associated with COVID-19 on day collection. From these, 1,405 positive cases identified. The distribution viral loads within these asymptomatic was indistinguishable what has been previously in symptomatic individuals. Regardless status,...