A5013945339- SARS-CoV-2 detection and testing
- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- SARS-CoV-2 and COVID-19 Research
- COVID-19 epidemiological studies
- COVID-19 and healthcare impacts
- Infection Control and Ventilation
- COVID-19 diagnosis using AI
- COVID-19 and Mental Health
- Respiratory viral infections research
- COVID-19 Clinical Research Studies
- Viral gastroenteritis research and epidemiology
University of Colorado Boulder
2020-2023
We analyze data from the fall 2020 pandemic response efforts at University of Colorado Boulder, where more than 72,500 saliva samples were tested for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) using qRT-PCR. All collected individuals who reported no symptoms associated with COVID-19 on day collection. From these, 1,405 positive cases identified. The distribution viral loads within these asymptomatic was indistinguishable what has been previously observed in symptomatic...
Here, we develop a simple molecular test for SARS-CoV-2 in saliva based on reverse transcription loop-mediated isothermal amplification. The has two steps: (1) heat with stabilization solution and (2) detect virus by incubating primer/enzyme mix. After incubation, samples containing the genome turn bright yellow. Because this is pH dependent, it can react falsely to some naturally acidic samples. We report unique protocols that rendered 295 healthy compatible test, producing zero false...
Abstract Here, we develop a simple molecular test for SARS-CoV-2 in saliva based on reverse transcription loop-mediated isothermal amplification (RT-LAMP). The has two steps: 1) heat with stabilization solution, and 2) detect virus by incubating primer/enzyme mix. After incubation, samples containing the genome turn bright yellow. Because this is pH dependent, it can react falsely to some naturally acidic samples. We report unique protocols that rendered 295 healthy compatible test,...
Abstract Background Over the course of COVID-19 pandemic, colleges and universities have focused on creating policies, such as mask mandates, to minimize transmission both their campuses in surrounding community. Adherence opinions about these policies remain largely unknown. Methods The Centers for Disease Control Prevention (CDC) developed a cross-sectional study, Mask Surveillance at Colleges Universities Project (MASCUP!), objectively inconspicuously measure rates use institutes higher...
Abstract We analyze data from the Fall 2020 pandemic response efforts at University of Colorado Boulder (USA), where more than 72,500 saliva samples were tested for SARS-CoV-2 using quantitative RT-PCR. All collected individuals who reported no symptoms associated with COVID-19 on day collection. From these, 1,405 positive cases identified. The distribution viral loads within these asymptomatic was indistinguishable what has been previously in symptomatic individuals. Regardless status,...
ABSTRACT The COVID-19 pandemic demonstrated the poor ability of body temperature to reliably identify SARS-CoV-2-infected individuals, an observation that has been made before in context other infectious diseases. While acute infection does not always cause fever, it drive host transcriptional responses as responds at site infection. These changes can occur both cells are directly harboring replicating pathogens and elsewhere receive a molecular signal is occurring. Here, we core set...