A5032564704- SARS-CoV-2 detection and testing
- Mosquito-borne diseases and control
- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- Viral Infections and Vectors
- interferon and immune responses
- Machine Learning in Bioinformatics
- SARS-CoV-2 and COVID-19 Research
- Studies on Chitinases and Chitosanases
- COVID-19 diagnosis using AI
- Viral Infections and Immunology Research
- RNA regulation and disease
- RNA Research and Splicing
- Vibrio bacteria research studies
- Cytokine Signaling Pathways and Interactions
- COVID-19 epidemiological studies
- COVID-19 Clinical Research Studies
- Virology and Viral Diseases
University of Colorado Boulder
2018-2021
Institut thématique Génétique, génomique et bioinformatique
2021
We analyze data from the fall 2020 pandemic response efforts at University of Colorado Boulder, where more than 72,500 saliva samples were tested for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) using qRT-PCR. All collected individuals who reported no symptoms associated with COVID-19 on day collection. From these, 1,405 positive cases identified. The distribution viral loads within these asymptomatic was indistinguishable what has been previously observed in symptomatic...
Human dengue viruses emerged from primate reservoirs, yet paradoxically does not reach high titers in models. This presents a unique opportunity to examine the genetics of spillover versus reservoir hosts. The virus 2 (DENV2) - encoded protease cleaves human STING, reducing type I interferon production and boosting viral humans. We find that both sylvatic (reservoir) universally cleave but STING primates implicated as species. special ability is thus specific humans few closely related ape...
Here, we develop a simple molecular test for SARS-CoV-2 in saliva based on reverse transcription loop-mediated isothermal amplification. The has two steps: (1) heat with stabilization solution and (2) detect virus by incubating primer/enzyme mix. After incubation, samples containing the genome turn bright yellow. Because this is pH dependent, it can react falsely to some naturally acidic samples. We report unique protocols that rendered 295 healthy compatible test, producing zero false...
RNase L–mediated shutoff of nuclear mRNA export limits viral protein synthesis and regulates antiviral cytokine production.
Abstract Here, we develop a simple molecular test for SARS-CoV-2 in saliva based on reverse transcription loop-mediated isothermal amplification (RT-LAMP). The has two steps: 1) heat with stabilization solution, and 2) detect virus by incubating primer/enzyme mix. After incubation, samples containing the genome turn bright yellow. Because this is pH dependent, it can react falsely to some naturally acidic samples. We report unique protocols that rendered 295 healthy compatible test,...
The Type I interferon response is an important first-line defense against viruses. In turn, viruses antagonize (i.e., degrade, mis-localize, etc.) many proteins in pathways. Thus, hosts and are locked evolutionary arms race for dominance of the pathway. As a result, genes pathways have experienced positive natural selection favor new allelic forms that can better recognize or escape viral antagonists. Here, we performed holistic analysis selective pressures acting on family. We initially...
Abstract We analyze data from the Fall 2020 pandemic response efforts at University of Colorado Boulder (USA), where more than 72,500 saliva samples were tested for SARS-CoV-2 using quantitative RT-PCR. All collected individuals who reported no symptoms associated with COVID-19 on day collection. From these, 1,405 positive cases identified. The distribution viral loads within these asymptomatic was indistinguishable what has been previously in symptomatic individuals. Regardless status,...
Abstract RNase L is widely thought to limit viral protein synthesis by cleaving host rRNA and mRNA, resulting in translation arrest mRNA degradation. Herein, we show that the mRNAs of dengue virus influenza A largely escape L-mediated decay, this permits production. However, activation arrests nuclear export, which strongly inhibits reduces cytokine Importantly, heterogeneous temporal nature export block individual cells sufficient production antiviral cytokines from transcriptionally...