A5071485045- Bat Biology and Ecology Studies
- Adipose Tissue and Metabolism
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Genomics and Chromatin Dynamics
- Plant Molecular Biology Research
- Chromosomal and Genetic Variations
- Spaceflight effects on biology
- CRISPR and Genetic Engineering
- Circadian rhythm and melatonin
- Adipokines, Inflammation, and Metabolic Diseases
- Effects of Environmental Stressors on Livestock
- RNA Research and Splicing
- Reproductive Biology and Fertility
- DNA Repair Mechanisms
- Sperm and Testicular Function
- Vector-borne infectious diseases
- Vector-Borne Animal Diseases
- Invertebrate Immune Response Mechanisms
- Advanced biosensing and bioanalysis techniques
- Pluripotent Stem Cells Research
- Animal Genetics and Reproduction
- Plant Virus Research Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Immune Cell Function and Interaction
- Ubiquitin and proteasome pathways
Kitasato University
2006-2025
The University of Tokyo
2016-2017
RIKEN BioResource Research Center
2015
Japan Science and Technology Agency
2014-2015
Obihiro University of Agriculture and Veterinary Medicine
2003
The variant histones TH2A and TH2B are abundant in the testis, but their roles spermatogenesis remain elusive. Here, we show that male mutant mice lacking both Th2a Th2b genes were sterile, with few sperm epididymis. In lack of was compensated for by overexpression H2B, whereas H2A not observed, indicating a decrease total histone level. Mutant exhibited two defects: incomplete release cohesin at interkinesis after meiosis I replacement during spermiogenesis. secondary spermatocytes...
The chipmunk hibernation-specific HP-27 gene is expressed specifically in the liver and has a CpG-poor promoter. To reveal how liver-specific transcription of regulated, we performed yeast one-hybrid screening cDNA library. A 5′-flanking sequence gene, extending from −170 to −140 containing an E-box (5′-CACGTG-3′), essential for HP-27. We used this as bait found that ubiquitously factor, USF (upstream stimulatory factor), bound E-box. In COS-7 cells, activated then bisulphite genomic...
Abstract Most vertebrate sex-determining genes (SDGs) emerge as neofunctionalized through duplication and/or mutation of ancestral that are involved with sexual differentiation. We previously demonstrated dm-W to be the SDG in African clawed frog Xenopus laevis and found a portion this gene emerged from masculinization dmrt1 after allotetraploidization by interspecific hybridization between two species around 17–18 Ma. has four exons consisting noncoding exon 1, dmrt1-derived 2 3, an orphan...
Abstract The differentiation of preadipocytes into adipocytes is controlled by several transcription factors, including peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT/enhancer-binding protein α (C/EBPα), which are known as master regulators adipogenesis. BCL11B a zinc finger-type factor that regulates the development skin central nervous immune systems. Here, we found was expressed in white adipose tissue (WAT), particularly subcutaneous WAT −/− mice had reduced amount WAT....
Abstract Mammalian hibernation is a seasonal phenomenon. The season consists of torpor periods with reduced body temperature (Tb), interrupted by euthermic arousal (interbout arousal, IBA). physiological changes associated are assumed to be under genetic control. However, the molecular mechanisms that govern hibernation-associated gene regulation still unclear. We found HSP70 transcription upregulated in liver nonhibernating (summer-active) chipmunks compared hibernating (winter-torpid)...
Many sex-determining genes (SDGs) were generated as neofunctionalized through duplication and/or mutation of gonadal formation-related genes. We previously identified dm-W an SDG in the African clawed frog Xenopus laevis and found that a partial masculinization gene dmrt1 created after allotetraploidization by interspecific hybridization. The allotetraploid species have two genes, dmrt1.L dmrt1.S. has four exons: dmrt1.S-derived exons (exons 2 3) other (noncoding exon 1 4). Our recent work...
Abstract The chipmunk hibernation-related protein 25 (HP-25) is involved in the circannual control of hibernation brain. liver-specific expression HP-25 gene repressed hibernating chipmunks under endogenous rhythms. However, molecular mechanisms that differentially regulate during nonhibernation and seasons are unknown. Here, we show hibernation-associated regulated epigenetically. Chromatin immunoprecipitation analyses revealed significantly less hepatocyte nuclear receptor HNF-4 bound to...
BCL11B is a zinc finger-type transcription factor that regulates the development of white adipose tissue (WAT), skin, central nervous system, and immune system. required for proper adipocyte differentiation, BCL11B-/- embryos at E19.5 have very low amounts subcutaneous WAT. Here, we demonstrated BCL11B+/- mice lower body weight than BCL11B+/+ mice, whereas expression adipogenic marker genes in WAT was comparable between mice. Histological analysis indicated fed high-fat diet much smaller...
During winter hibernation, a diverse range of small mammals can enter prolonged torpor. They spend the nonhibernation season as homeotherm but hibernation heterotherm. In season, chipmunks (Tamias asiaticus) cycle regularly between 5 and 6 days-long deep torpor with body temperature (Tb) to 7 °C interbout arousal ∼20 h, during which, their Tb returns normothermic level. Here, we investigated Per2 expression in liver elucidate regulation peripheral circadian clock mammalian hibernator. mice,...
The chipmunk hibernation-related proteins (HPs) HP-20 and HP-27 are components of a 140-kDa complex that dramatically decreases in the blood during hibernation. genes expressed specifically liver downregulated hibernating chipmunks. Hibernation-associated physiological changes assumed to be under genetic control. Therefore, elucidate molecular mechanisms hibernation, here we examined behind altered gene expression nonhibernating versus Chromatin immunoprecipitation (ChIP) analyses revealed...
In certain tetraploid species resulting from interspecific hybridization, one parent's subgenome is known to selectively undergo DNA loss. The molecular mechanisms behind this remain unclear. our study, we compared the genomes of a standard diploid with two allotetraploid Xenopus genus, both possessing L (longer) and S (shorter) homoeologous subgenomes. We observed substantial gene losses intergenic deletions in subgenomes species. Gene were around 1,000 3,000 for 4,000 6,000 S, especially...
Doublesex and mab-3 related transcription factor (DMRT) 1, commonly found in all vertebrates, regulates the of genes involved masculinization maintenance gonadal somatic cells and/or germline cell development. DMRT1 has a DNA-binding domain called DM regulatory region. Unlike former, there is little knowledge about latter This study aimed to identify activation regions from four species: humans mice (mammals), leopard geckos (reptiles), medaka (teleost fish), adding perspectives on...