A5047326288- Light effects on plants
- Circadian rhythm and melatonin
- Algal biology and biofuel production
- Photosynthetic Processes and Mechanisms
- Photoreceptor and optogenetics research
- Epigenetics and DNA Methylation
- GABA and Rice Research
- Plant Genetic and Mutation Studies
- Spaceflight effects on biology
- Genetic Mapping and Diversity in Plants and Animals
- Micro and Nano Robotics
- Plant Molecular Biology Research
- Amino Acid Enzymes and Metabolism
- Health, Environment, Cognitive Aging
- Advanced Proteomics Techniques and Applications
- Genetics and Neurodevelopmental Disorders
- Microtubule and mitosis dynamics
- Craniofacial Disorders and Treatments
- Polyamine Metabolism and Applications
- Erythrocyte Function and Pathophysiology
- Genetics, Aging, and Longevity in Model Organisms
- Birth, Development, and Health
- Plant Virus Research Studies
- Psychological and Temporal Perspectives Research
- Genetic and Kidney Cyst Diseases
Kitasato University
2024-2025
Nagoya University
2012-2022
National Center for Gene Research
2006-2013
Bio-oriented Technology Research Advancement Institution
2006
Kobe University
2003
Yamaguchi University
2003
Tohoku Institute of Technology
2003
Kindai University
1989
Cell division in many mammalian tissues is associated with specific times of day, but just how the circadian clock controls this timing has not been clear. Here, we show regenerating liver (of mice) that expression cell cycle–related genes turn modulate active Cyclin B1-Cdc2 kinase, a key regulator mitosis. Among these genes, wee1 was directly regulated by molecular components clockwork. In contrast, clockwork oscillated independently cycle single cells. Thus, intracellular can control...
Individual cellular clocks in the suprachiasmatic nucleus (SCN), circadian center, are integrated into a stable and robust pacemaker with period length of about 24 hours. We used real-time analysis gene expression to show synchronized rhythms clock transcription across hundreds neurons within mammalian SCN organotypic slice culture. Differentially phased neuronal topographically arranged SCN. A protein synthesis inhibitor set all cell same initial phase and, after withdrawal, intrinsic...
The molecular bases of circadian clocks have been studied in animals, fungi, bacteria, and plants, but not eukaryotic algae. To establish a new model for analysis the clock, here we identified large number components system unicellular alga Chlamydomonas reinhardtii by systematic forward genetic approach. We isolated 105 insertional mutants that exhibited defects period, phase angle, and/or amplitude rhythms bioluminescence derived from luciferase reporter gene their chloroplast genome....
Abstract Light is essential for photosynthesis, but the amounts of light that exceed an organism’s assimilation capacity can result in oxidative stress and even cell death. Plants microalgae have developed a photoprotective response mechanism, qE, dissipates excess energy as thermal energy. In green alga Chlamydomonas reinhardtii , qE regulated by light-inducible proteins, pathway from perception to not fully understood. Here, we show transcription factors CONSTANS Nuclear Factor Ys (NF-Ys)...
Microalgal triacylglycerol (TAG), a promising source of biofuel, is induced upon nitrogen starvation (-N), but the proteins and genes involved in this process are poorly known. We performed isobaric tagging for relative absolute quantification (iTRAQ)-based quantitative proteomics to identify Chlorella with modulated expression under short-term -N. Out 1736 soluble 2187 membrane-associated identified, 288 56, respectively, were differentially expressed Gene analysis on select confirmed same...
Chloroplast-encoded genes, like nucleus-encoded exhibit circadian expression. How the clock exerts its control over chloroplast gene expression, however, is poorly understood. To facilitate study of we developed a codon-optimized firefly luciferase for Chlamydomonas reinhardtii as real-time bioluminescence reporter and introduced it into genome. The strain correlated well with expression pattern satisfied all three criteria rhythms. Moreover, period rhythm was lengthened in per mutants,...
Although the circadian clock is a self-sustaining oscillator having periodicity of nearly 1 d, its period length not necessarily 24 h. Therefore, daily adjustment (i.e., resetting) an essential mechanism for to adapt environmental changes. One major cues this resetting light. In unicellular green alga Chlamydomonas reinhardtii , reset by blue/green and red However, underlying molecular mechanisms remain largely unknown. study, using protein-luciferase fusion reporters, we found that level...
Arabidopsis thaliana has long been the model plant of choice for elucidating mechanisms circadian clock. Recently, relevant results have accumulated in other species green lineages, including algae. This mini-review describes a comparison mechanism A. clock to those alga Chlamydomonas reinhardtii and moss Physcomitrella patens, focusing on commonalities divergences subsystems The potential such an approach from evolutionary viewpoint is discussed.
The methyl cycle is a universal metabolic pathway providing groups for the methylation of nuclei acids and proteins, regulating all aspects cellular physiology. We have previously shown that inhibition in mammals strongly affects circadian rhythms. Since clocks evolved early during evolution operate organisms across tree life, we sought to determine whether link between two also conserved. Here, show biological rhythms species ranging from unicellular algae humans, separated by more than 1...
Abstract In photosynthetic organisms, photoprotection to avoid overexcitation of photosystems is a prerequisite for survival. Green algae have evolved light-inducible photoprotective mechanisms mediated by genes such as light-harvesting complex stress-related ( LHCSR ). Studies on the light-dependent regulation expression in green alga Chlamydomonas reinhardtii revealed that photoreceptors blue light (phototropin) and ultraviolet perception (UVR8) play key roles initiating signal...
The green alga Chlamydomonas reinhardtii shows various light responses in behavior and physiology. One such photoresponse is the circadian clock, which can be reset by external signals to entrain its oscillation daily environmental cycles. In a previous report, we suggested that light-induced degradation of clock protein ROC15 trigger Chlamydomonas. However, signaling pathways this process remained unclear. Here, screened for mutants show abnormal diurnal rhythms, including at dawn, using...
The circadian clocks in chlorophyte algae have been studied two model organisms, Chlamydomonas reinhardtii and Ostreococcus tauri. These studies revealed that the include some genes are homologous to those of angiosperm clock. However, genetic network architectures largely unknown, especially C. reinhardtii. In this study, using as a model, we characterized RHYTHM OF CHLOROPLAST (ROC) 75, clock gene encoding putative GARP DNA-binding transcription factor similar proteins LUX ARRHYTHMO (LUX,...
Strong light intensity leads to harmful overexcitation of the photosystems in green algae. In Chlamydomonas reinhardtii, LHCSR3 is required for rapid protective response known as energy-dependent quenching (qE). Because majority photoacclimation analysis has been conducted under controlled laboratory conditions, physiological responses natural environmental changes such light/dark cycles have not examined detail. Regarding fitness higher plants and microalgae, light-dark represent a major...
Following the previous report (MATSUO and NAKAJIMA 1960), authors made a study in gene-analysis of some early heading mutants induced from Norin-No. 8 by X-ray. The materials were as follows: M1 strain: 1O days earlier than original variety under natural condition, but 7 later high temperatire short day condition. M2 40 earlier; non-sensible strain photoperiodic response on heading, viridies type. M4 20days decreasing sensibility, short-culm stiff-stow F2 segregations for time among hybrids...
For the purpose of studying on heading characters in rice plants genetically, authors collected 14 mutant strains which were induced from 5 varieties by X-ray or β-ray (cf. table 1), and investigated their characteristics others, espesially, photoperiodic sensibility minimum duration. The results as follows: ( 1 ) Generally speaking, earliness lateness time these comparing with original variety wewe mainly attributable to decreasing increasing sensibility, slightly, connected duration too,...
Physiological changes in the photosynthesis, respiration and cell division of Chlamydomonas reinhardtii, a freshwater green alga, response to adenine nucleotides were investigated. In advance this investigation, two nucleotides, di(2'-O-methyl)-cyclic bis(3'-5')diadenylic acid (1) its N-benzoyl-protected analog 2 synthesized from commercially available adenosine phosphoramidite. The respective analogs significantly promoted (cell number) C. reinhardtii strains 137c mt+ BR mt+. Moreover, they...
In certain tetraploid species resulting from interspecific hybridization, one parent's subgenome is known to selectively undergo DNA loss. The molecular mechanisms behind this remain unclear. our study, we compared the genomes of a standard diploid with two allotetraploid Xenopus genus, both possessing L (longer) and S (shorter) homoeologous subgenomes. We observed substantial gene losses intergenic deletions in subgenomes species. Gene were around 1,000 3,000 for 4,000 6,000 S, especially...
Doublesex and mab-3 related transcription factor (DMRT) 1, commonly found in all vertebrates, regulates the of genes involved masculinization maintenance gonadal somatic cells and/or germline cell development. DMRT1 has a DNA-binding domain called DM regulatory region. Unlike former, there is little knowledge about latter This study aimed to identify activation regions from four species: humans mice (mammals), leopard geckos (reptiles), medaka (teleost fish), adding perspectives on...
An amendment to this paper has been published and can be accessed via a link at the top of paper.