A5015965391- Animal Virus Infections Studies
- SARS-CoV-2 and COVID-19 Research
- Bacteriophages and microbial interactions
- Vector-Borne Animal Diseases
- Vector-borne infectious diseases
- Antibiotic Resistance in Bacteria
- Plant Pathogens and Fungal Diseases
- Evolutionary Game Theory and Cooperation
- Toxic Organic Pollutants Impact
- Infectious Diseases and Mycology
- Atmospheric chemistry and aerosols
- Veterinary medicine and infectious diseases
- Microbial Community Ecology and Physiology
- Legume Nitrogen Fixing Symbiosis
- Plant and Fungal Interactions Research
- Vibrio bacteria research studies
- Virology and Viral Diseases
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Bacterial Genetics and Biotechnology
- Insect symbiosis and bacterial influences
- Genetics, Bioinformatics, and Biomedical Research
- Plant Pathogenic Bacteria Studies
- Bat Biology and Ecology Studies
- Genomics and Phylogenetic Studies
- Radio, Podcasts, and Digital Media
University of Regina
2017-2025
We present the first high-resolution determination of transcriptome architecture in priority pathogen Acinetobacter baumannii. Pooled RNA from 16 laboratory conditions was used for differential RNA-seq (dRNA-seq) to identify 3731 transcriptional start sites (TSS) and 110 small RNAs, including identification A. baumannii sRNAs encoded at 3′ end coding genes. Most were conserved among sequenced genomes, but only weakly or absent other species. Single nucleotide mapping TSS enabled prediction...
Pathogen life history theory assumes a positive relationship between pathogen load in host tissues and transmission. Empirical evidence for this is surprisingly rare due to the difficulty of measuring transmission many pathogens. The comparative method, where common experimentally infected with set strains, powerful approach investigating relationships validity such experimental estimates strain-specific greatly enhanced if they can predict population strain structure nature. Borrelia...
ABSTRACT Antimicrobial resistance (AMR) is a global threat. The identification and characterization of novel genes integral to AMR surveillance. erm (55) gene was originally identified through whole genome sequencing macrolide-resistant strains Mycobacterium chelonae . annotated as ribosomal methyltransferase, but its role determinant macrolide not formally demonstrated. Three alleles have now been documented. plasmid-borne P , the transposon-associated T chromosomal encoded C exhibit ≈82%...
Public health emergencies like SARS, MERS, and COVID-19 have prioritized surveillance of zoonotic coronaviruses, resulting in extensive genomic characterization coronavirus diversity bats. Sequencing viral genomes directly from animal specimens remains a laboratory challenge, however, most bat coronaviruses been characterized solely by PCR amplification small regions the best-conserved gene. This has resulted limited phylogenetic resolution left genetic factors relevant to threat assessment...
The plant growth-promoting rhizobacterium Delftia acidovorans RAY209 is capable of establishing strong root attachment during early development at 7 days post-inoculation. transcriptional response was measured using RNA-seq (day 2) and sustained 7) colonization canola plants, capturing differentiation from a medium-suspended cell state to strongly root-attached state. Transcriptomic data collected in an identical manner interaction with soybean roots explore the putative this globally...
Abstract Perfluoroalkyl and polyfluoroalkyl substances (PFAS) are environmental contaminants of concern. We previously described biodegradation two PFAS that represent components transformation products aqueous film-forming foams (AFFF), 6:2 fluorotelomer sulfonamidoalkyl betaine (6:2 FTAB) sulfonate FTSA), by Gordonia sp. strain NB4-1Y. To identify genes involved in the breakdown these compounds, transcriptomic response NB4-1Y was examined when grown on FTAB, FTSA, a non-fluorinated analog...
Abstract The COVID‐19 pandemic prompted a transition to remote delivery of courses that lack immersive hands‐on research experiences for undergraduate science students, resulting in scientific skills gap. In this report, we present an option inclusive and authentic, experience all students can perform off‐campus. Biology semester‐long (13 weeks) sophomore plant physiology course participated at‐home laboratory designed study the impacts nitrogen addition on growth rates root nodulation by...
Changing climates are allowing the geographic expansion of ticks and their animal hosts, increasing risk Borrelia -caused zoonoses in Canada. However, little is known about genomic diversity from west Canadian Rockies tick vectors Ixodes pacificus , auritulus angustus . Here, we report whole-genome shotgun sequences 51 isolates multiple species collected on a range hosts between 1993 2016, located primarily coastal British Columbia. The bacterial represented three different Lyme...
Deinococcus sp. strain UR1, a resilient bacterium isolated from the surface of stainless steel sign located on University Regina campus in Saskatchewan, Canada, was sequenced to 56-fold coverage produce 73 contigs with consensus length 4,472,838 bp and G+C content 69.37%.
ABSTRACT Public health emergencies like SARS, MERS, and COVID-19 have prioritized surveillance of zoonotic coronaviruses, resulting in extensive genomic characterization coronavirus diversity bats. Sequencing viral genomes directly from animal specimens remains a laboratory challenge, however, most bat coronaviruses been characterized solely by PCR amplification small regions the best-conserved gene. This has resulted limited phylogenetic resolution left genetic factors relevant to threat...
A BSTRACT We present the first high-resolution determination of transcriptome architecture in priority pathogen Acinetobacter baumannii . Pooled RNA from 16 laboratory conditions was used for differential RNA-seq (dRNA-seq) to identify 3731 transcriptional start sites (TSS) and 110 small RNAs, including identification A. sRNAs encoded at 3’ end coding genes. Most were conserved among sequenced genomes, but only weakly or absent other species. Single nucleotide mapping TSS enabled prediction...
Ignavigranum ruoffiae is a rare human pathogen. Strain CPL 242382-20 was isolated in Manitoba, Canada, from breast cyst. Whole-genome sequencing performed with the Oxford Nanopore Technologies MinION and Illumina MiSeq platforms. The circular chromosome 1,949,382 bp 39.68% G+C content 1,765 protein-coding genes.