A5061121091- Genetic diversity and population structure
- Aquatic Invertebrate Ecology and Behavior
- Marine Bivalve and Aquaculture Studies
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Chromosomal and Genetic Variations
- Identification and Quantification in Food
- Aquaculture Nutrition and Growth
- Reproductive biology and impacts on aquatic species
- Genetic and phenotypic traits in livestock
- Fish Ecology and Management Studies
- Animal Genetics and Reproduction
- Marine Ecology and Invasive Species
- Evolution and Genetic Dynamics
- Mollusks and Parasites Studies
- Genomics and Phylogenetic Studies
- Isotope Analysis in Ecology
- Sperm and Testicular Function
- Fish Biology and Ecology Studies
- RNA and protein synthesis mechanisms
- Environmental Toxicology and Ecotoxicology
- Forensic and Genetic Research
- Marine Biology and Ecology Research
- Marine and fisheries research
- MicroRNA in disease regulation
- Advanced biosensing and bioanalysis techniques
Universidad de Cádiz
2019-2025
Universidade da Coruña
2011-2021
University of Southern California
2014
Sex determination (SD) shows huge variation among fish and a high evolutionary rate, as illustrated by the Pleuronectiformes (flatfishes). This order is characterized its adaptation to demersal life, compact genomes diversity of SD mechanisms. Here, we assembled Solea senegalensis genome, flatfish great commercial value, into 82 contigs (614 Mb) combining long- short-read sequencing, which were next scaffolded using highly dense genetic map (28,838 markers, 21 linkage groups), representing...
Fish exhibit diverse mechanisms of sex differentiation and determination, shaped by both external internal influences, often regulated distinct DNA methylation patterns responding to environmental changes. In S. senegalensis aquaculture, reproductive issues in captivity pose significant challenges, particularly the lack fertilization capabilities captive-bred males, hindering genetic improvement measures. This study analyzed transcriptomic profiles gonadal tissue from groups differing...
Abstract The clam Ruditapes decussatus is commercially important in southwestern Europe, suffering from population decline and hybridization with exotic Manila ( R. philippinarum ). Previous studies intronic markers showed a genetic subdivision of the species three races (Atlantic, West Mediterranean, Adriatic‐Aegean). However, detailed to help management main production areas southwest Europe are missing. We have analyzed eight Atlantic two Mediterranean populations Spanish coasts using 14...
Abstract Studies of linkage and mapping have advanced genetic biological knowledge for over 100 years. In addition to their growing role, today, in phenotypes genotypes, dense maps can help validate genome assemblies. Previously, we showed that 40% scaffolds the first assembly Pacific oyster Crassostrea gigas were chimeric, containing single nucleotide polymorphisms (SNPs) different groups. Here, merge 14 constructed SNPs generated from genotyping-by-sequencing (GBS) methods with five,...
The internal transcribed spacer (ITS) region of ribosomal DNA (ITS1, 5.8S rRNA gene and ITS2) constitutes one the most widely applied molecular markers in phylogenetic studies species differentiation. Here, ITS cockles Cerastoderma edule from NW Spain C. glaucum Mediterranean Baltic Seas was characterized its variation examined. length 762–783 bp 226–251 bp; gene, 158–161 ITS2, 305–325 bp) GC content ranged between 57% 60% 52–62%; 56–57%; 61–63%). All individuals showed among repeats, mostly...
Solea senegalensis is a flatfish belonging to the Soleidae family within Pleuronectiformes order. It has karyotype of 2n = 42 (FN 60; 6M + 4 SM 8 St 24 T) and XX/XY system. The first pair metacentric chromosomes been proposed as proto sex-chromosome originated by Robertsonian fusion between acrocentric chromosomes. In order elucidate possible evolutionary origin this chromosome 1, studies genomic synteny were carried out with eight fish species. A total 88 genes annotated 14 BACs located in...
Abstract The wedge clam, Donax trunculus (Linnaeus, 1758), is an ecologically and economically important species found in the warm waters of Atlantic Mediterranean areas. has been suffering from a severe population decline some wild stocks Iberian Peninsula, genetic monitoring populations can provide valuable information to support conservation measures management policies. In this study, mitochondrial 16S rDNA (16S) cytochrome b (Cytb) gene variation was examined at 18 localities across...
Exon-primed intron-crossing PCR was used on the European commercial scallops Aequipecten opercularis and Mimachlamys varia to characterize introns of four nuclear genes identify DNA markers useful for population studies. The primers yielded expected product, except those lysozyme gene that failed work in M. amplified a fragment proteasome subunit (APSM) A. opercularis. According sequences characterized, has at least calmodulin genes, one arginine kinase two β-tubulin, five, one,...
Microsatellites are one of the most popular markers in genetic studies but typically they need to be isolated and characterized de novo for each species. In this work, a genomic library enriched trinucleotide motif was constructed identify polymorphic microsatellite loci Aequipecten opercularis, scallop species commercially fished Europe, examine level variation differentiation samples from Spain Northern Ireland. Sequencing 83 clones led identification 30 microsatellite-containing sequences...
Doubly uniparental inheritance (DUI) is a particular mitochondrial DNA mode reported in number of bivalves. DUI species show two types mtDNA, one transmitted from females to daughters and sons (F mitotype) another males (M mitotype). In Veneridae, the existence has been investigated several but it was found only them. this study, we obtained partial sequences rrnL, cytb cox1 genes Polititapes rhomboides NW Spain demonstrated heteroplasmy males, as expected under DUI. F M mitotypes showed...
Solea senegalensis aquaculture production has experienced a great increase in the last decade and, consequently, genome knowledge of species is gaining attention. In this sense, obtaining high-density mapping could offer clues to improvement those aspects not resolved so far. present article, review and new processed data have allowed obtain BAC-based cytogenetic map S. beside analysis sequences such BAC clones achieve integrative data. A total 93 were used localize chromosome complement 588...