A5068918871- Cell Adhesion Molecules Research
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Lymphoma Diagnosis and Treatment
- Galectins and Cancer Biology
- Chronic Lymphocytic Leukemia Research
- Immunotherapy and Immune Responses
- Ubiquitin and proteasome pathways
- Lipid Membrane Structure and Behavior
- Glycosylation and Glycoproteins Research
- CAR-T cell therapy research
- Protein Degradation and Inhibitors
- Photosynthetic Processes and Mechanisms
- Studies on Chitinases and Chitosanases
- Peptidase Inhibition and Analysis
- Hemoglobin structure and function
- Erythrocyte Function and Pathophysiology
- Monoclonal and Polyclonal Antibodies Research
- interferon and immune responses
- Fungal and yeast genetics research
- Microbial Metabolic Engineering and Bioproduction
- Electrohydrodynamics and Fluid Dynamics
Radboud University Nijmegen
2017-2024
Radboud University Medical Center
2017-2024
Radboud Institute for Molecular Life Sciences
2017-2020
University Medical Center
2018
Cancer Genomics Centre
2013
Oncode Institute
2010
The Netherlands Cancer Institute
2010
Diffuse large B-cell lymphoma (DLBCL) is a common aggressive form of Non-Hodgkin lymphoma. Tetraspanin CD37 highly expressed on mature B cells and being studied as therapeutic target for NHL, including DLBCL. DuoHexaBody-CD37 biparatopic antibody with an E430G hexamerization-enhancing mutation targeting two non-overlapping epitopes shown to promote complement-dependent cytotoxicity. However, the impact direct cytotoxic signaling has not yet been studied. Here we demonstrate that induces...
Specific degradation of proteins is essential for virtually all cellular processes and carried out predominantly by the proteasome. The proteasome important clearance damaged proteins. Damaged accumulate over time excess may aggregate induce death old cells. In yeast, localization changes dramatically during aging, possibly in response to altered activity requirements. We followed two key parameters this process: distribution proteasomes nuclear cytosolic compartments formation cytoplasmic...
CD20 is a four-transmembrane protein expressed at the surface of B cells from late pro-B to memory cells, with exception plasma cells. Its expression pattern makes it an attractive therapeutic target for different cell malignancies and autoimmune diseases. Despite clinical success CD20-targeting antibodies, biology still not well understood. We investigated binding partners in membrane human using immunoprecipitation followed by mass spectrometry analysis. identified molecular interaction...
Lipidation of transmembrane proteins regulates many cellular activities, including signal transduction, cell-cell communication, and membrane trafficking. However, how lipidation at different sites in a protein affects structure function remains elusive. Here, using native mass spectrometry we determined that wild-type human tetraspanins CD9 CD81 exhibit nonstochastic distributions bound acyl chains. We revealed its three most frequent lipidated suffices for EWI-F binding, while...
Abstract Proteins are not static entities. They highly mobile, and their steady-state levels achieved by a balance between ongoing synthesis degradation. The dynamic properties of protein can have important consequences for its function. For example, when is degraded replaced newly synthesized one, posttranslational modifications lost need to be reincorporated in the new molecules. Protein stability mobility also relevant duplication macromolecular structures or organelles, which involves...
Cell migration is central to evoking a potent immune response. Dendritic cell (DC) lymph nodes dependent on the interaction of C-type lectin-like receptor 2 (CLEC-2; encoded by gene
Tetraspanin proteins play an important role in many cellular processes as they are key organizers of different receptors on the plasma membrane. Most tetraspanins highly glycosylated at their large extracellular loop; however, little is known about function tetraspanin glycosylation immune cells. In this study we investigated effects CD37 and CD53, two for humoral immunity. Broad cell-specific repertoires N-glycosylated CD53 were observed human B We generated mutants analyzed localization,...
Tetraspanins, including CD53 and CD81, are four-transmembrane proteins that affect the membrane organization to regulate cellular processes migration, proliferation, signaling. However, it is unclear how organizing function of tetraspanins regulated at molecular level. Here, we investigated whether recently proposed "open" "closed" conformations nanoscale plasma B cells. We generated conformational mutants (F44E) CD81 (4A, E219Q) represent conformation, respectively. Surface expression these...
Abstract Tetraspanin proteins play an important role in many cellular processes as they are key organizers of different receptors on the plasma membrane. Most tetraspanins highly glycosylated at their large extracellular loop, but function this post-translational modification remains largely unstudied. In study we investigated effects glycosylation CD37 and CD53, two for humoral immunity. Broad cell-specific repertoires N-glycosylated CD53 were observed human B cells. We generated mutants...
Abstract Cell migration is central to evoke a potent immune response. Dendritic cell (DC) lymph nodes dependent on the interaction of C-type lectin-like receptor 2 (CLEC-2) expressed by DCs, with podoplanin node stromal cells (LNSCs). However, underlying molecular mechanisms which CLEC-2 influences DC remain elusive. Here, we show that CLEC-2-dependent tightly controlled tetraspanin CD37, membrane-organizing protein. Our findings demonstrate specific between and CD37. Myeloid lacking CD37 (...