A5025681236- Lymphoma Diagnosis and Treatment
- Chronic Lymphocytic Leukemia Research
- Viral-associated cancers and disorders
- Cancer Genomics and Diagnostics
- CAR-T cell therapy research
- Brain Metastases and Treatment
- Cancer Immunotherapy and Biomarkers
- Glioma Diagnosis and Treatment
- Monoclonal and Polyclonal Antibodies Research
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Molecular Biology Techniques and Applications
- Sarcoma Diagnosis and Treatment
- Cutaneous Melanoma Detection and Management
- Ocular Oncology and Treatments
- Immunodeficiency and Autoimmune Disorders
- T-cell and B-cell Immunology
- Genetic factors in colorectal cancer
- Connexins and lens biology
- Genomic variations and chromosomal abnormalities
- Melanoma and MAPK Pathways
- Renal Diseases and Glomerulopathies
- Blood properties and coagulation
- Genetic Neurodegenerative Diseases
- Soft tissue tumor case studies
Radboud University Nijmegen
2016-2025
Radboud University Medical Center
2016-2025
Radboud Institute for Molecular Life Sciences
2003-2023
University Medical Center
2015-2022
University Hospital and Clinics
2019
Data Harbor (United States)
2015
Erasmus MC
2014
Institute of Cancer Research
2013
Cancer Research UK
2013
Cancer Research UK Manchester Institute
2013
alpha-Crystallin, composed of the subunits alpha A and B, is a major vertebrate eye lens protein, accomplishing structural role in maintaining stability transparency. Both also occur low amounts outside lens, where their precise function unknown. They are structurally related to small heat-shock proteins (HSPs), increasing evidence indicates that they have functional similarities with HSPs. To extend our insight into these relationships, mouse HSP (HSP25) was compared bovine...
Epithelioid hemangioendothelioma is a malignant, often indolent vascular tumor which occurs at various anatomic sites. Based on reciprocal translocation t (1;3)(p36;q25), consistent WWTR1-CAMTA1 fusion gene has been found. An alternate YAP1-TFE3 detected in small and distinct subset of cases.Thirty-nine tumors, from 24 females 15 males with an age range 9-85 years, were located soft tissue (head neck [8], trunk [5], upper extremities [3], lower [2], mediastinal [1], paratesticular [1]),...
Amplicon-based next-generation sequencing (NGS) of immunoglobulin (IG) and T-cell receptor (TR) gene rearrangements for clonality assessment, marker identification quantification minimal residual disease (MRD) in lymphoid neoplasms has been the focus intense research, development application. However, standardization validation a scientifically controlled multicentre setting is still lacking. Therefore, IG/TR assay design, including bioinformatics, was performed within EuroClonality-NGS...
Summary Introduction: Refractory coeliac disease (RCD) is a rare syndrome with poor prognosis, defined by malabsorption due to gluten‐related enteropathy after initial or subsequent failure of strict gluten‐free diet and exclusion any disorder mimicking disease. Patients methods : Nineteen patients were included treated. Based on intraepithelial T‐lymphocyte(IEL) phenotyping, recorded as having RCD type I normal IELs, II phenotypically immature IELs lack characteristic T‐cell markers....
Primary melanocytic neoplasms of the central nervous system (CNS) are uncommon derived from melanocytes that normally can be found in leptomeninges. They cover a spectrum malignancy grades ranging low-grade melanocytomas to lesions intermediate and overtly malignant melanomas. Characteristic genetic alterations this group have not yet been identified. Using direct sequencing, we investigated 19 primary CNS (12 melanocytomas, 3 intermediate-grade 4 melanomas) for hotspot oncogenic mutations...
Solitary fibrous tumor is a mesenchymal of fibroblastic type, which can affect any region the body. Recently, recurrent gene fusion NAB2-STAT6 has been identified as molecular hallmark. The leads to EGR1 activation and transcriptional deregulation EGR1-dependent target genes driving event in initiation SFT. In this study, we report clinicopathologic RT-PCR findings evaluated expression STAT6 protein cohort 28 SFTs. patients with median age 54 years were included SFTs originating at different...
One of the hallmarks B lymphoid malignancies is a cell clone characterized by unique footprint clonal immunoglobulin (IG) gene rearrangements that serves as diagnostic marker for clonality assessment. The EuroClonality/BIOMED-2 assay currently gold standard analyzing IG heavy chain (IGH) and κ light (IGK) suspected lymphomas. Here, EuroClonality-NGS Working Group presents multicentre technical feasibility study novel approach involving next-generation sequencing (NGS) IGH IGK loci highly...
The study of immunoglobulins and T cell receptors using next-generation sequencing has finally allowed exploring immune repertoires responses in their immense variability complexity. Unsurprisingly, analysis interpretation is a highly convoluted task.We thus implemented ARResT/Interrogate, web-based, interactive application. It can organize filter large amounts immunogenetic data by numerous criteria, calculate several relevant statistics, present results the form multiple interconnected...
Assessment of clonality, marker identification and measurement minimal residual disease (MRD) immunoglobulin (IG) T cell receptor (TR) gene rearrangements in lymphoid neoplasms using next-generation sequencing (NGS) is currently under intensive development for use clinical diagnostics. So far, however, there a lack suitable quality control (QC) options with regard to standardisation metrics ensure robust application such approaches. The EuroClonality-NGS Working Group has therefore...
In most pathology laboratories worldwide, formalin-fixed paraffin embedded (FFPE) samples are the only tissue specimens available for routine diagnostics. Although commercial kits diagnostic molecular testing becoming available, of current tests laboratory-based assays. Thus, there is a need standardized procedures in pathology, starting from extraction nucleic acids. To evaluate methods extracting acids FFPE tissues, 13 European laboratories, participating to FP6 program IMPACTS...
Background:Inflammatory bowel disease (IBD) patients on thiopurine therapy are at increased risk of Epstein–Barr virus (EBV)-associated lymphomas. This is frequently detected in the intestinal mucosa IBD and may cause a wide spectrum lymphoproliferations similar to post-transplantation lymphoproliferative disorders (PTLDs). We aimed assess whether histological aberrations aid predicting EBV presence correlate assessment load with outcome IBD.
In 2010, a new subtype of salivary gland cancer (SGC), (mammary analogue) secretory carcinoma (SC), was defined, characterized by the ETV6-NTRK3 fusion gene. As clinical behavior and outcome data this histological tumor are still sparse, we aimed to describe clinicopathological course series translocation positive SC patients.We re-evaluated pathological diagnosis subset SGCs, diagnosed in 4 8 Dutch head neck centers. Subsequently, tumors with morphological resemblance were tested for gene...
Follicular lymphoma (FL), except grade 3B, is characterized by the chromosomal translocation t(14;18)(q32;q21), which results in over-expression of Bcl-2 protein. Ten per-cent all FLs, however, do not show protein expression with standard immunohistochemistry using a monoclonal antibody against residues 41-54 In this study, biological background 18 Bcl-2-negative FL cases I, II, or IIIa was investigated immunohistochemical staining and western blot analysis alternative antibodies....