A5042721662- Genetics, Aging, and Longevity in Model Organisms
- Circadian rhythm and melatonin
- Genetic and Kidney Cyst Diseases
- Analytical Chemistry and Chromatography
- Genetic Neurodegenerative Diseases
- Chemical Thermodynamics and Molecular Structure
- Inorganic and Organometallic Chemistry
- Mitochondrial Function and Pathology
- Thermal and Kinetic Analysis
- DNA Repair Mechanisms
- Neuroendocrine regulation and behavior
- Microtubule and mitosis dynamics
- Solid-state spectroscopy and crystallography
- Inorganic Fluorides and Related Compounds
- Chemical Synthesis and Characterization
- Radioactive element chemistry and processing
- Renal and related cancers
- Chemical Reaction Mechanisms
- Catalysis and Oxidation Reactions
- Organometallic Compounds Synthesis and Characterization
- Molecular Spectroscopy and Structure
- Various Chemistry Research Topics
- CRISPR and Genetic Engineering
- Protist diversity and phylogeny
- Spaceflight effects on biology
Amsterdam University Medical Centers
2024
Erasmus University Rotterdam
2012-2022
Erasmus MC
2008-2019
Dalhousie University
2016
Clausthal University of Technology
2016
Radboud University Nijmegen
1992-2011
University Medical Center Groningen
2010
Meertens Institute
2009
University of Groningen
2007
Oncode Institute
1997-2004
Myotonic dystrophy (DM) is the most common inherited neuromuscular disease in adults, with a global incidence of 1 8000 individuals. DM an autosomal dominant, multisystemic disorder characterized primarily by myotonia and progressive muscle weakness. Genomic complementary DNA probes that map to 10-kilobase Eco RI genomic fragment from human chromosome 19q13.3 have been used detect variable length polymorphism individuals DM. Increases size allele patients are now shown be due increased...
Heterochromatin protein 1 (HP1) family members are chromatin-associated proteins involved in transcription, replication, and chromatin organization. We show that HP1 isoforms HP1-α, HP1-β, HP1-γ recruited to ultraviolet (UV)-induced DNA damage double-strand breaks (DSBs) human cells. This response requires the chromo shadow domain of is independent H3K9 trimethylation detect UV DSBs. Loss results high sensitivity light ionizing radiation nematode Caenorhabditis elegans, indicating essential...
Vasopressin- and oxytocin-related neuropeptides are key regulators of animal physiology, including water balance reproduction. Although these also modulate social behavior cognition in mammals, the mechanism for influencing behavioral plasticity evolutionary origin effects not well understood. Here, we present a functional vasopressin- oxytocin-like signaling system nematode Caenorhabditis elegans. Through activation its receptor NTR-1, vasopressin/oxytocin-related neuropeptide, designated...
Nucleotide Excision Repair (NER), which removes a variety of helix-distorting lesions from DNA, is initiated by two distinct DNA damage-sensing mechanisms. Transcription Coupled (TCR) damage the active strand transcribed genes and depends on SWI/SNF family protein CSB. Global Genome (GGR) present elsewhere in genome recognition XPC/RAD23/Centrin2 complex. Currently, it not well understood to what extent both pathways contribute maintenance cell survival developing organism exposed UV light....
The mutation causing myotonic dystrophy (DM) has recently been identified as an unstable CTG trinucleotide repeat located in the 3' untranslated region of a gene encoding for protein with putative serine-threonine kinase activity. In this report we present genomic sequences human and murine DM gene. A comparison these each other known cDNA from both species, led us to predict translation initiation codon, well determine organization Several polymorphisms within have identified, PCR assays...
In the cilia of nematode Caenorhabditis elegans, anterograde intraflagellar transport (IFT) is mediated by two kinesin-2 complexes, kinesin II and OSM-3 kinesin. These complexes function together in middle segments, whereas alone mediates distal segments. Not much known about mechanisms that compartmentalize or how both kinesins coordinated. Here, we identify DYF-5, a conserved MAP kinase plays role these processes. Fluorescence microscopy EM revealed dyf-5 loss-of-function (lf) animals are...
Primary cilia are important sensory organelles. They exist in a wide variety of lengths, which could reflect different cell-specific functions. How cilium length is regulated unclear, but it probably involves intraflagellar transport (IFT), transports protein complexes along the ciliary axoneme. Studies various organisms have identified small, conserved family ros-cross hybridizing kinases (RCK) as regulators length. Here we show that Intestinal Cell Kinase (ICK) and MAPK/MAK/MRK overlapping...
Epidermolysis bullosa simplex (EBS) is a mechanobullous genodermatosis that may be caused by mutations in the genes KRT5 and KRT14 encoding basal epidermal keratins 5 (K5) 14 (K14). Three main clinical subtypes of EBS exist, differing onset, distribution severity skin blistering. Previous reports suggest correlation between location mutation associated phenotype.The prevalence KRT5/KRT14 genotype-phenotype largest tissue-confirmed population investigated.KRT5 genomic DNA cDNA sequences 76...
While naïve Caenorhabditis elegans individuals are attracted to 0.1–200 mM NaCl, they become strongly repelled by these NaCl concentrations after prolonged exposure 100 NaCl. We call this behavior gustatory plasticity. Here, we show that C. displays avoidance of low only when pre-exposure is combined with a negative stimulus, e.g., repellent, or in the absence food. By testing serotonin and/or dopamine signaling mutants and rescue exogenously supplying neurotransmitters, found play role...