A5006708816- Blood groups and transfusion
- Erythrocyte Function and Pathophysiology
- Hemoglobinopathies and Related Disorders
- Platelet Disorders and Treatments
- Blood disorders and treatments
- Celiac Disease Research and Management
- Microscopic Colitis
- Parvovirus B19 Infection Studies
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Immunodeficiency and Autoimmune Disorders
- Herpesvirus Infections and Treatments
- Biochemical Analysis and Sensing Techniques
- Blood donation and transfusion practices
- RNA and protein synthesis mechanisms
- RNA regulation and disease
- Food Science and Nutritional Studies
- Enterobacteriaceae and Cronobacter Research
- Iron Metabolism and Disorders
- Caveolin-1 and cellular processes
- Thyroid Cancer Diagnosis and Treatment
- Helicobacter pylori-related gastroenterology studies
- Cell Adhesion Molecules Research
- Hepatitis B Virus Studies
Faculdade de Medicina de São José do Rio Preto
2019-2024
Colsan - Associação Beneficente de Coleta de Sangue
2012-2021
Universidade Federal de São Paulo
2016-2019
Company of Biologists
2015
As an alternative to phenotyping, large-scale DNA-based assays, which are feasible for high-throughput donor red blood cell typing, were developed determination of group polymorphisms. However, genotyping platforms based on these technologies still expensive and the inclusion single nucleotide polymorphisms analysis alleles depend manufacturer's determination. To overcome this limitation in order develop assay enable screening rare donors, we a SNaPshot nine related antigens that difficult...
Serologic resolution of Rh discrepancies due to partial D or weak phenotypes is a frequent problem encountered during routine typing that can be solved by RHD genotyping because it provides better characterization these variants. The objective the current study was develop algorithms for identification variants in multiethnic populations based on logic sequence molecular tests using large number atypical RhD specimens. Thus, total 360 blood samples with antigen expression were analyzed. A...
SUMMARY BACKGROUND The high homology and opposite orientation of RH genes promote rearrangements between them generate a large number RHD RHCE variants which can be inherited together. Searching RHD‐CE genotypes predicting partial antigens in donors is interest order to find more closely matched for African descent patients. This study aimed evaluate molecular approach search RhCE cohort individuals with altered expression D antigen determine the association variant alleles Brazilian blood...
Celiac disease is a permanent intolerance induced by gluten, which expressed T-cell mediated enteropathy, and has high prevalence in the general population. There evidence of strong genetic predisposition to celiac disease.To determine markers HLA-DQ2 HLA-DQ8 blood donors from São Paulo measure human recombinant tissue transglutaminase antibody IgA class positive donors.A total 404 city Jundiaí were included study signed informed consent form. Information regarding diarrhea, constipation...
First-degree relatives (FDRs) of 47 outpatients with celiac disease (CD) answered a questionnaire about symptoms related to CD and were investigated for human leukocyte antigen (HLA)-DQ2, DQB102 homozygosis, DQ8 alleles. Genetically susceptible individuals tested antitransglutaminase antibody immunoglobulin A. Seropositive FDR underwent small bowel biopsies.From 114 FDR, 74.5% (n = 85) positive DQ2, DQ8, or both haplotypes. Homozygosity was found in 11.4% 13) individuals. Three previously...
Rhnull is a rare phenotype characterized by the loss of Rh antigen expression. This can be related to several molecular backgrounds. In this study, we show novel allele in Brazilian pregnant woman encoding due change RHAG exon2 c.310C>T, which leads premature stop codon (Gln104Stop).
Providing blood units for patients with an antibody to a high-prevalence antigen or multiple common antibodies is constant challenge the banks. Finding compatible donor requires extensive screening, incurs large amount of investment. In this article, we share our experience organizing rare inventory limited resources, include strategy used finding donors, and difficulties found during implementation approach results obtained.
Background and objectives Weak D phenotypes with very low antigen densities DEL phenotype may not be detected in RhD typing routine could typed as D‐negative, leading to alloimmunization of D‐negative recipients. The present study aimed investigate the presence RHD ‐positive genotypes blood donors by an automated system using solid‐phase methodology a confirmatory test. Methods Two screenings were performed different selected donor populations. For first screening, we 1403 samples regardless...
Vel is a high frequency blood group antigen and its alloantibody involved in haemolytic transfusion reactions. After elucidation of the molecular basis Vel-negative phenotype defined by 17-base pair deletion SMIM1, genotyping has been technique choice to identify phenotype, investigations have contributed explain expression variability. The present study was aimed at screening for negative donors characterising genetic changes found Brazilian with altered expression.Molecular SMIM1*64_80del...
Fig. S1. Electropherogram of the 2 K0 samples studied. Control are on top figure and probands bellow. (A) Proband 1 is homozygous for a c.71G>A in Exon that leads to premature stop codon (Trp24Stop). (B) mutation g>a at position +1 Intron 16, alters splice donor site, following coding region until (TAA). Please note: The publisher not responsible content or functionality any supporting information supplied by authors. Any queries (other than missing content) should be directed corresponding...
Several centers have selected Black donors to prevent Rh alloimmunization of patients with sickle cell disease (SCD). As the Brazilian population is considered very admixed and race definition by self-declaration questionable, this study aimed compare RHCE diversity among SCD groups blood define which group would be adequate red supply for SCD.We compared allele frequencies between four donors: self-declared (SDB), predominant African genetic markers (AAM), weak D expression (WDD), random...
Abstract Background Among the alleles of ABO system, cisAB and B(A) are most intriguing due to their ability encode a glycosyltransferase that can synthesize both A B antigens. This dual activity leads formation AB phenotype, even in presence O allele; resolution is achieved by molecular analyses. Case Presentation Methods We describe herein Brazilian family which mother (M42.1) group father (M42.2) have two children (M42.3 M42.4). Serological characterization involved H phenotyping tests...
Wra is a low-incidence antigen, which antithetical to the high prevalence red blood cell Wrb. Anti-Wra naturally occurring antibody that found in approximately 1–2% of donors. The aim this study was determine frequency and anti-Wra Brazilian A total 1662 donors were molecularly analyzed using SNaPshot methodology WR*A/B alleles predict antigen. To detect anti-Wra, samples from 1049 gel test with Wr(a+) cells. serum treated dithiothreitol (DTT) immunoglobulin classes. Immunoglobulin (Ig)-G...
Abstract Background Red blood cell (RBC) alloimmunization is a complication of patients with sickle disease (SCD) and it has greater impact on pregnancy, leading to risk hemolytic the newborn reducing availability for pregnant women. This study proposed evaluate antigen matching transfusion protocols, aiming reduce RBC in Brazilian female SCD. Methods Samples from SCD (153) self‐declared Afro‐Brazilian donors (307) were genotyped antigens RH variants investigated. The needs during 1‐year...
Abstract Background and Objectives Gerbich (GE) blood group system carries high‐frequency antigens the absence of them leads to rare phenotypes: GE:−2,3,4, GE:−2,−3,4 GE:−2,−3,−4. Their serological differentiation is limited misclassification phenotypes may occur, but this can be avoided by molecular characterization. This study aimed characterize background responsible for in Brazilian population. Materials Methods We selected eight samples from patients with anti‐Ge, six their relatives...