Mark A. Williams
A5101820378- SARS-CoV-2 and COVID-19 Research
- Pediatric Urology and Nephrology Studies
- Urological Disorders and Treatments
- vaccines and immunoinformatics approaches
- COVID-19 Clinical Research Studies
- Bacillus and Francisella bacterial research
- Animal Virus Infections Studies
- Monoclonal and Polyclonal Antibodies Research
- Viral Infections and Immunology Research
- Urinary Tract Infections Management
- SARS-CoV-2 detection and testing
- Enzyme Structure and Function
- Immunotherapy and Immune Responses
- Ureteral procedures and complications
- Infrared Thermography in Medicine
- Scientific Computing and Data Management
- Retinal and Optic Conditions
- Kidney Stones and Urolithiasis Treatments
- Adolescent and Pediatric Healthcare
- Urologic and reproductive health conditions
- Advanced X-ray Imaging Techniques
- Viral gastroenteritis research and epidemiology
- Head and Neck Surgical Oncology
- Influenza Virus Research Studies
- Gastrointestinal disorders and treatments
Diamond Light Source
2013-2024
University of Tennessee Health Science Center
2005-2015
Ducks Unlimited
2010
U.S. Army Center for Health Promotion and Preventive Medicine
2010
Children's Medical Center
1994-2007
Le Bonheur Children's Hospital
1994-2007
University of Tennessee at Knoxville
1994-2007
University of Cincinnati
2002
Cincinnati Children's Hospital Medical Center
2002
On 24th November 2021, the sequence of a new SARS-CoV-2 viral isolate Omicron-B.1.1.529 was announced, containing far more mutations in Spike (S) than previously reported variants. Neutralization titers Omicron by sera from vaccinees and convalescent subjects infected with early pandemic Alpha, Beta, Gamma, or Delta are substantially reduced, failed to neutralize. Titers against boosted third vaccine doses high both vaccinated individuals those Delta. Mutations knock out reduce...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has undergone progressive change, with variants conferring advantage rapidly becoming dominant lineages, e.g., B.1.617. With apparent increased transmissibility, variant B.1.617.2 contributed to the current wave of infection ravaging Indian subcontinent and been designated a concern in United Kingdom. Here we study ability monoclonal antibodies convalescent vaccine sera neutralize B.1.617.1 B.1.617.2, complement this structural...
The Omicron lineage of SARS-CoV-2, which was first described in November 2021, spread rapidly to become globally dominant and has split into a number sublineages. BA.1 dominated the initial wave but been replaced by BA.2 many countries. Recent sequencing from South Africa's Gauteng region uncovered two new sublineages, BA.4 BA.5, are taking over locally, driving wave. BA.5 contain identical spike sequences, although closely related BA.2, they further mutations receptor-binding domain their...
Highlights•Despite similar RBD mutations, P.1 is easier to neutralize than B.1.351•P.1, B.1.351, and B.1.1.7 partially or fully escape most VH3-53 antibodies•mAb 222 (VH3-53) retains neutralization against all three variants•Neutralization restored in chimeric antibodies with mAb LCSummaryTerminating the SARS-CoV-2 pandemic relies upon pan-global vaccination. Current vaccines elicit neutralizing antibody responses virus spike derived from early isolates. However, new strains have emerged...
SARS-CoV-2 has caused over 2 million deaths in little a year. Vaccines are being deployed at scale, aiming to generate responses against the virus spike. The scale of pandemic and error-prone replication is leading appearance mutant viruses potentially escape from antibody responses. Variant B.1.1.7, now dominant UK, with increased transmission, harbors 9 amino acid changes spike, including N501Y ACE2 interacting surface. We examine ability B.1.1.7 evade elicited by natural infection or...
Antibodies are crucial to immune protection against SARS-CoV-2, with some in emergency use as therapeutics. Here, we identify 377 human monoclonal antibodies (mAbs) recognizing the virus spike and focus mainly on 80 that bind receptor binding domain (RBD). We devise a competition data-driven method map RBD sites. find although antibody sites widely dispersed, neutralizing is focused, nearly all highly inhibitory mAbs (IC
Highly transmissible Omicron variants of SARS-CoV-2 currently dominate globally. Here, we compare neutralization BA.1, BA.1.1, and BA.2. BA.2 RBD has slightly higher ACE2 affinity than BA.1 reduced by vaccine serum, possibly associated with its increased transmissibility. Neutralization differences between sub-lineages for mAbs (including therapeutics) mostly arise from variation in residues bordering the binding site; however, more distant mutations S371F (BA.2) R346K (BA.1.1) markedly...
It remains largely mysterious how the genomes of non-enveloped eukaryotic viruses are transferred across a membrane into host cell. Picornaviruses simple models for such viruses, and initiate this uncoating process through particle expansion, which reveals channels internal capsid proteins viral genome presumably exit particle, although has not been clearly seen until now. Here we present atomic structure an intermediate major human picornavirus pathogen CAV16, VP1 partly extruded from...
Summary On the 24 th November 2021 sequence of a new SARS CoV-2 viral isolate spreading rapidly in Southern Africa was announced, containing far more mutations Spike (S) than previously reported variants. Neutralization titres Omicron by sera from vaccinees and convalescent subjects infected with early pandemic as well Alpha, Beta, Gamma, Delta are substantially reduced or fail to neutralize. Titres against boosted third vaccine doses high cases both vaccinated Delta. Mutations knock out...
Variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have caused successive global waves infection. These variants, with multiple mutations in the spike protein, are thought to facilitate escape from natural and vaccine-induced immunity often increase affinity for ACE2. The latest variant cause concern is BA.2.75, identified India where it now dominant strain, evidence wider dissemination. BA.2.75 derived BA.2 contains four additional receptor-binding domain (RBD). Here,...
In November 2021, Omicron BA.1, containing a raft of new spike mutations, emerged and quickly spread globally. Intense selection pressure to escape the antibody response produced by vaccines or severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection then led rapid succession sub-lineages with waves BA.2 BA.4/5 infection. Recently, many variants have such as BQ.1 XBB, which carry up 8 additional receptor-binding domain (RBD) amino acid substitutions compared BA.2. We describe...
Abstract The rapid evolution of SARS-CoV-2 is driven in part by a need to evade the antibody response face high levels immunity. Here, we isolate spike (S) binding monoclonal antibodies (mAbs) from vaccinees who suffered vaccine break-through infections with Omicron sub lineages BA.4 or BA.5. Twenty eight potent are isolated and characterised functionally, some cases structurally. Since emergence BA.4/5, has continued accrue mutations S protein, understand this characterize neutralization...
Herein, we describe the development of a novel dual air-bearing fixed-χ diffractometer for beamline I19 at Diamond Light Source. The is designed to facilitate rapid data collections possible with Dectris Pilatus 2M pixel-array photon-counting detector, while allowing remote operation in conjunction robotic sample changer. sphere-of-confusion made as small practicably possible, through use air-bearings both ω and φ axes. design construction new instrument described detail an accompanying...
Summary Terminating the SARS-CoV-2 pandemic relies upon pan-global vaccination. Current vaccines elicit neutralizing antibody responses to virus spike derived from early isolates. However, new strains have emerged with multiple mutations: P.1 Brazil, B.1.351 South Africa and B.1.1.7 UK (12, 10 9 changes in respectively). All mutations ACE2 binding site having a virtually identical triplet: E484K, K417N/T N501Y, which we show confer similar increased affinity for ACE2. We that, surprisingly,...
No AccessJournal of UrologyVesicoureteral Reflux1 Oct 2005ULTRASOUND SCREENING OF ASYMPTOMATIC SIBLINGS CHILDREN WITH VESICOURETERAL REFLUX: A LONG-TERM FOLLOWUP STUDY DANA W. GIEL, H. NORMAN NOE, and MARK A. WILLIAMS GIELDANA GIEL More articles by this author , NOEH. NOE WILLIAMSMARK View All Author Informationhttps://doi.org/10.1097/01.ju.0000176596.87624.a3AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail...
Antibodies are crucial to immune protection against SARS-CoV-2 with some in emergency use as therapeutics. Here we identified 377 human monoclonal antibodies (mAbs) recognizing the viral spike, and focused on 80 which bind receptor binding domain (RBD). By mapping antigenic sites using a unique computational methodology comparing inhibitory activity, show that widely dispersed, but neutralizing epitopes highly focused. Nearly all potent mAbs (IC50< 0.1mg/ml) block interaction, although one...
Diurnal incontinence secondary to dysfunctional voiding is a problem that commonly encountered by the pediatric urologist. We have observed reflux more common in these children and hypothesized urinary tract infection would influence rate of as well significant abnormalities, such renal scarring. prospectively evaluated 308 incontinent with cystourethrogram upper studies. Of patients 162 (53%) had previous culture proved infection, while 146 (47%) no history infection. A total 90 (29%) an...
Abstract Commercially developed monoclonal antibodies (mAb) have been effective in the prevention or treatment of SARS-CoV-2 infection 1-3 but rapid antigenic evolution Omicron sub-lineages has reduced their activity 4-8 and they are no longer licensed for use many countries. Here, we isolate spike binding from vaccinees who suffered vaccine break-through infections with sublineages BA.4/5. We find that it is possible targeting highly mutated regions to recover broad through allosteric...
You have accessJournal of UrologyPediatrics: Stone Disease1 Apr 20101051 UROLITHIASIS IN PATIENTS TREATED FOR CHILDHOOD CANCER: DOES ALTERED CALCIUM METABOLISM PLAY A ROLE? Kathleen Kieran, Dana W. Giel, Andrew M. Davidoff, and Mark A. Williams KieranKathleen Kieran More articles by this author , GielDana Giel DavidoffAndrew Davidoff WilliamsMark View All Author Informationhttps://doi.org/10.1016/j.juro.2010.02.2163AboutPDF ToolsAdd to favoritesDownload CitationsTrack...