- Insect symbiosis and bacterial influences
- Genomics and Phylogenetic Studies
- Parasite Biology and Host Interactions
- Evolution and Genetic Dynamics
- Protist diversity and phylogeny
- Entomopathogenic Microorganisms in Pest Control
- Bacterial Infections and Vaccines
- Marine Sponges and Natural Products
- Antibiotic Resistance in Bacteria
- Microbial Community Ecology and Physiology
- Escherichia coli research studies
- Vector-borne infectious diseases
- Insect Resistance and Genetics
- Viral gastroenteritis research and epidemiology
- Wastewater Treatment and Nitrogen Removal
- Pharmaceutical and Antibiotic Environmental Impacts
- Insects and Parasite Interactions
- Bacterial Genetics and Biotechnology
- Synthetic Organic Chemistry Methods
- Receptor Mechanisms and Signaling
- Microbial Natural Products and Biosynthesis
- Trypanosoma species research and implications
- Respiratory viral infections research
- Cell Image Analysis Techniques
- Mathematical Biology Tumor Growth
Toscana Life Sciences
2024-2025
University of South Bohemia in České Budějovice
2020-2021
Sewanee: The University of the South
2019-2020
National Research Council
2016-2019
UNSW Sydney
2017-2019
Water Research Institute
2016-2019
Abstract Despite the development of several cultivation methods, rate discovery microorganisms that are yet-to-be cultivated outpaces isolating and cultivating novel species in laboratory. Furthermore, no current technique is capable selectively specific bacterial taxa or phylogenetic groups independently morphological physiological properties. Here, we developed a new method to isolate living bacteria solely based on their 16S rRNA gene sequence. We showed can survive modified version...
Kissing bugs (Triatominae) are blood-feeding insects best known as the vectors of Trypanosoma cruzi, causative agent Chagas' disease. Considering high epidemiological relevance these vectors, their biology and bacterial symbiosis remains surprisingly understudied. While previous investigations revealed generally low individual complexity but among-individual variability triatomine microbiomes, any consistent microbiome determinants have not yet been identified across multiple Triatominae...
Abstract In this review, we present the efforts made so far in developing effective solutions to prevent infections caused by seven major respiratory pathogens: influenza virus, syncytial virus (RSV), severe acute syndrome coronavirus 2 (SARS-CoV-2), Bordetella pertussis, Streptococcus pneumoniae (pneumococcus), Mycobacterium tuberculosis, and Pseudomonas aeruginosa. Advancements driven recent disease 2019 (COVID-19) crisis have largely focused on viruses, but prophylactic for bacterial...
SUMMARY The silent pandemic caused by antimicrobial resistance (AMR) requires innovative therapeutic approaches. Human monoclonal antibodies (mAbs), which are among the most transformative, safe and effective drugs in oncology autoimmunity, rarely used for infectious diseases not yet AMR. Here we applied an antigen-agnostic strategy to isolate extremely potent human mAbs against Klebsiella pneumoniae (Kp) sequence type 147 (ST147), a hypervirulent pandrug-resistant clonotype is spreading...
Abstract The phylogenetic diversity of symbiotic bacteria in sucking lice suggests that have a complex history symbiont acquisition, loss, and replacement throughout their evolution. These processes resulted the establishment different, phylogenetically distant as obligate mutualists different louse groups. By combining metagenomics amplicon screening across several populations three species (members genera Polyplax Hoplopleura ) we describe novel lineage related to Neisseria Snodgrassella ,...
Among the bacterivorous protists, heterotrophic nanoflagellates (HNFs) are considered to be main grazers of bacteria in freshwaters due their size-selective grazing. In this work, we assessed change a riverine bacterial community controlled incubations, where HNFs' predation pressure was initially released through filtration. Filtration did not prevent passage cysts, which grew enrichments afterwards. Data on composition were gathered by Catalyzed Reporter Deposition Fluorescent situ...
Introduction Shigella is the etiologic agent of a bacillary dysentery known as shigellosis, which causes millions infections and thousands deaths worldwide each year due to ’s unique lifestyle within intestinal epithelial cells. Cell adhesion/invasion assays have been extensively used not only identify targets mediating host-pathogen interaction, but also evaluate ability -specific antibodies reduce virulence. However, these are time-consuming labor-intensive fail assess differences at...
<title>Abstract</title> The silent pandemic caused by antimicrobial resistance (AMR) requires innovative therapeutic approaches. Human monoclonal antibodies (mAbs), which are among the most transformative, safe and effective drugs in oncology autoimmunity, rarely used for infectious diseases not yet AMR. Here we applied an antigen-agnostic strategy to isolate extremely potent human mAbs against Klebsiella pneumoniae (Kp) sequence type 147 (ST147), a hypervirulent pandrug-resistant clonotype...
Glyphosate-based herbicides are widely used for several crops, such as transgenic soybean and forestry. The aim of this study was to determine the effect glyphosate on community structure riverine bacterioplankton evaluate potential degrade herbicide. River water which 13C-labelled (10, 100µgL–1) added or not (control) incubated 6 days at temperature measured in situ (20°C). Significant differences composition, assessed by microfluidics-based automated ribosomal intergenic spacer analysis,...
Abstract Phylogenetic diversity of symbiotic bacteria in sucking lice suggests that have experienced a complex history symbiont acquisition, loss, and replacement during their evolution. By combining metagenomics amplicon screening across several populations two louse genera ( Polyplax Hoplopleura ) we describe novel lineage related to Neisseria Snodgrassella , show its’ independent origin within dynamic microbiomes. While the genomes these symbionts are highly similar both genera,...
Abstract Background Kissing bugs (Triatominae) are blood-feeding insects best known as the vectors of Trypanosoma cruzi , causative agent Chagas’ disease. Considering high epidemiological relevance these vectors, their biology and bacterial symbiosis remains surprisingly understudied. While previous investigations revealed generally low individual complexity but among-individual variability triatomine microbiomes, any consistent microbiome determinants have not yet been identified across...
Phylogenetic diversity of symbiotic bacteria in sucking lice suggests that have experienced a complex history symbiont acquisition, loss, and replacement during their evolution. By combining metagenomics amplicon screening across several populations two louse genera (Polyplax Hoplopleura) we describe novel lineage related to Neisseria Snodgrassella, show its independent origin within dynamic microbiomes. While the genomes these symbionts are highly similar both genera, respective...