A5065167048- Microbial Metabolic Engineering and Bioproduction
- SARS-CoV-2 and COVID-19 Research
- Enzyme Catalysis and Immobilization
- Aquaculture disease management and microbiota
- Immunotherapy and Immune Responses
- Viral gastroenteritis research and epidemiology
- Enzyme Structure and Function
- Microbial Community Ecology and Physiology
- COVID-19 Clinical Research Studies
- Genomics and Phylogenetic Studies
- Enzyme Production and Characterization
- Monoclonal and Polyclonal Antibodies Research
- Biofuel production and bioconversion
- Microbial Natural Products and Biosynthesis
- Aquaculture Nutrition and Growth
- Animal Virus Infections Studies
- Invertebrate Immune Response Mechanisms
- Advanced Proteomics Techniques and Applications
- vaccines and immunoinformatics approaches
- Antibiotic Resistance in Bacteria
- Marine Sponges and Natural Products
- Escherichia coli research studies
- Enterobacteriaceae and Cronobacter Research
- Polar Research and Ecology
- Chemistry and Chemical Engineering
Toscana Life Sciences
2020-2024
UiT The Arctic University of Norway
2014-2019
Institute of Protein Biochemistry
2008-2016
National Research Council
2012-2016
National Academies of Sciences, Engineering, and Medicine
2012
Abstract The emergence of SARS-CoV-2 variants is jeopardizing the effectiveness current vaccines and limiting application monoclonal antibody-based therapy for COVID-19 (refs. 1,2 ). Here we analysed memory B cells five naive convalescent people vaccinated with BNT162b2 mRNA vaccine to investigate nature cell antibody response at single-cell level. Almost 6,000 were sorted, over 3,000 produced antibodies against spike protein more than 400 neutralized original virus first identified in...
The continuous evolution of SARS-CoV-2 generated highly mutated variants able to escape natural and vaccine-induced primary immunity. administration a third mRNA vaccine dose induces secondary response with increased protection. Here we investigate the longitudinal neutralizing antibody in four donors after three doses at single-cell level. We sorted 4100 spike protein specific memory B cells identifying 350 antibodies. increases neutralization potency breadth against all as observed hybrid...
The enormous potential in diversity of the marine life is still not fully exploited due to difficulty culturing many microorganisms under laboratory conditions. In this mini-review we underlined importance using an omic technique, such as metagenomics, access uncultured majority microbial communities. We report examples several hydrolytic enzymes and natural products isolated by functional sequenced-based function- screening strategies assisted new high-throughput DNA sequencing technology...
The use of metagenomics in enzyme discovery constitutes a powerful approach to access genomes unculturable community microorganisms and isolate novel valuable biocatalysts for wide range biotechnological pharmaceutical fields. Here we present esterase gene (lip3) identified by functional screening three fosmid metagenomic libraries, constructed from marine sediment samples. sequenced positive revealed an 281 amino acids with similarity class 3 lipases. 3D modeling Lip3 was generated homology...
SARS-CoV-2 vaccines, administered to billions of people worldwide, mitigate the effects COVID-19 pandemic, however little is known about molecular basis antibody cross-protection emerging variants, such as Omicron BA.1, its sublineage BA.2, and other coronaviruses. To answer this question, 276 neutralizing monoclonal antibodies (nAbs), previously isolated from seronegative seropositive donors vaccinated with BNT162b2 mRNA vaccine, were tested for neutralization against BA.1 BA.2 SARS-CoV-1...
The population of Atlantic cod (Gadus morhua), also known as Northeast Arctic cod, migrating or simply "skrei," lives mainly in the Barents Sea and Svalbard waters migrates annual cycles to Norwegian coast order spawn eggs during late winter. It is world's largest distinct from coastal (or "fjord" cod). Despite biological, economic, cultural importance current knowledge on associated microbiota very limited. Using shotgun metagenomics metaproteomics approaches, we present here gut...
Abstract Severe acute respiratory syndrome 2 Omicron BA.4 and BA.5 are characterized by high transmissibility ability to escape natural vaccine induced immunity. Here we test the neutralizing activity of 482 human monoclonal antibodies isolated from people who received two or three mRNA doses vaccinated after infection. The variants neutralized only approximately 15% antibodies. Remarkably, target mainly receptor binding domain Class 1/2, while infection recognize mostly 3 epitope region...
The glucuronoyl esterase enzymes of wood-degrading fungi (Carbohydrate Esterase family 15; CE15) form part the hemicellulolytic and cellulolytic enzyme systems that break down plant biomass, have possible applications in biotechnology. Homologous are predicted genomes several bacteria, however these been much less studied than their fungal counterparts. Here we describe recombinant production biochemical characterization a bacterial CE15 denoted MZ0003, which was identified by silico...
ABSTRACT In the absence of approved drugs or vaccines, there is a pressing need to develop tools for therapy and prevention Covid-19. Human monoclonal antibodies have very good probability being safe effective SARS-CoV-2 infection disease. Here we describe screening PBMCs from seven people who survived Covid-19 isolate human against SARS-CoV-2. Over 1,100 memory B cells were single-cell sorted using stabilized prefusion form spike protein incubated two weeks allow natural production...
SUMMARY The silent pandemic caused by antimicrobial resistance (AMR) requires innovative therapeutic approaches. Human monoclonal antibodies (mAbs), which are among the most transformative, safe and effective drugs in oncology autoimmunity, rarely used for infectious diseases not yet AMR. Here we applied an antigen-agnostic strategy to isolate extremely potent human mAbs against Klebsiella pneumoniae (Kp) sequence type 147 (ST147), a hypervirulent pandrug-resistant clonotype is spreading...
Abstract The marine Arctic isolate Halomonas sp. R5-57 was sequenced as part of a bioprospecting project which aims to discover novel enzymes and organisms from low-temperature environments, with potential uses in biotechnological applications. Phenotypically, exhibits high salt tolerance over wide range temperatures has extra-cellular hydrolytic activities several substrates, indicating it secretes may function salinity conditions. Genome sequencing identified the genes involved...
We have investigated the biotechnological potential of Arctic marine bacteria for their ability to produce a broad spectrum cold‐active enzymes. Marine exhibiting these features are great interest both fundamental research and industrial applications. Macrobiota, water sediment samples been collected during 2010 2011 expeditions around Lofoten Svalbard islands. Bacteria were isolated from this material identified through 16S rRNA gene sequence analysis purpose establishing culture collection...
ABSTRACT The continuous evolution of SARS-CoV-2 generated highly mutated variants, like omicron BA.1 and BA.2, able to escape natural vaccine-induced primary immunity 1,2 . administration a third dose mRNA vaccines induces secondary response with increased protection. We investigated, at single-cell level, the longitudinal neutralizing antibody in four donors after three doses 3 A total 4,100 spike protein specific memory B cells were single cell sorted 350 antibodies identified....
The family 15 carbohydrate esterase (CE15) MZ0003, which derives from a marine Arctic metagenome, has broader substrate scope than other members of this family. Here we report the crystal structure reveals that residues comprising catalytic triad differ previously-characterized fungal homologs, and resolves three large loop regions are unique to bacterial sub-clade. CE15, includes Asp 332 as its third member, closely resembles 1 esterases (CE1), despite overall lower structural similarity...
A salt-tolerant esterase, designated H9Est, was identified from a metagenomic library of the Karuola glacier. H9Est gene comprised 1071 bp and encoded polypeptide 357 amino acids with molecular mass 40 kDa. Sequence analysis revealed that belonged to family IV bacterial lypolitic enzyme. overexpressed in Escherichia coli purified enzyme showed hydrolytic activity towards p-nitrophenyl esters carbon chain 2 8. The optimal esterase at 40°C pH 8.0 retained its some miscible organic solvents...
SUMMARY Human monoclonal antibodies are safe, preventive and therapeutic tools, that can be rapidly developed to help restore the massive health economic disruption caused by Covid-19 pandemic. By single cell sorting 4277 SARS-CoV-2 spike protein specific memory B cells from 14 survivors, 453 neutralizing were identified 220 of them expressed as IgG. Up 65,9% monoclonals neutralized wild type virus at a concentration >500 ng/mL, 23,6% in range 100 - 500 ng/mL 9,1% had neutralization...