A5003122560- Enzyme Production and Characterization
- Microbial Community Ecology and Physiology
- Enzyme Structure and Function
- Genomics and Phylogenetic Studies
- Glycosylation and Glycoproteins Research
- Microbial Natural Products and Biosynthesis
- Vibrio bacteria research studies
- Bacterial Genetics and Biotechnology
- Enzyme Catalysis and Immobilization
- Biofuel production and bioconversion
- Microbial Metabolic Engineering and Bioproduction
- biodegradable polymer synthesis and properties
- Microplastics and Plastic Pollution
- Biocrusts and Microbial Ecology
- Advanced Cellulose Research Studies
- Marine Biology and Ecology Research
- Protein Structure and Dynamics
- Marine Ecology and Invasive Species
- Bacteriophages and microbial interactions
- Plant Pathogens and Fungal Diseases
- RNA and protein synthesis mechanisms
- Marine Sponges and Natural Products
- Carbon dioxide utilization in catalysis
- Pharmaceutical and Antibiotic Environmental Impacts
- Fungal Biology and Applications
UiT The Arctic University of Norway
2014-2023
Norwegian University of Life Sciences
2023
NORCE Norwegian Research Centre
2008
Cyanobacteria belonging to the genus Nostoc comprise free-living strains and also facultative plant symbionts. Symbiotic can enter into symbiosis with taxonomically diverse range of host plants. Little is known about genomic changes associated evolutionary transition from symbiont. Here, we compared genomes derived 11 symbiotic isolated different plants infer phylogenetic relationships between strains. Phylogenetic reconstructions 89 Nostocales showed that a broad range, entering epiphytic...
The use of metagenomics in enzyme discovery constitutes a powerful approach to access genomes unculturable community microorganisms and isolate novel valuable biocatalysts for wide range biotechnological pharmaceutical fields. Here we present esterase gene (lip3) identified by functional screening three fosmid metagenomic libraries, constructed from marine sediment samples. sequenced positive revealed an 281 amino acids with similarity class 3 lipases. 3D modeling Lip3 was generated homology...
ABSTRACT Terrestrial symbiotic cyanobacteria of the genus Nostoc exhibit a large potential for production bioactive natural products nonribosomal peptide, polyketide, and ribosomal peptide classes, yet most biosynthetic gene clusters are silent under conventional cultivation conditions. In present study, we utilized high-density approach recently developed phototrophic bacteria to rapidly generate biomass filamentous up density 400 g (wet weight)/liter. Unexpectedly, integrated...
ABSTRACT Marine fungi remain poorly covered in global genome sequencing campaigns; the 1000 fungal genomes (1KFG) project attempts to shed light on diversity, ecology and potential industrial use of overlooked resolved taxa. This study characterizes three marine fungi: Emericellopsis sp. TS7, wood-associated Amylocarpus encephaloides algae-associated Calycina marina. These species were sequenced their genomic features, biosynthetic phylogenetic placement using multilocus data. C. marina...
Abstract Background Several members of the bacterial Halomonadacea family are natural producers polyhydroxyalkanoates (PHA), which promising materials for use as biodegradable bioplastics. Type-strain species Cobetia designated PHA positive, and recent studies have demonstrated relatively high production a few strains within this genus. Industrially relevant may therefore be present among uncharacterized or less explored members. In study, we characterized in two marine strains. We further...
Endonuclease I is a periplasmic or extracellular enzyme present in many different Proteobacteria. The endA gene encoding endonuclease from the psychrophilic and mildly halophilic bacterium Vibrio salmonicida mesophilic brackish water cholerae have been cloned, over-expressed Escherichia coli, purified. A comparison of enzymatic properties shows large differences NaCl requirements, optimum pH, temperature stability catalytic efficiency two proteins. V. EndA typical cold-adapted features such...
Adenovirus infections in immunocompromised patients are associated with high mortality rates. Currently, there no effective anti-adenoviral therapies available. It is well known that actinobacteria can produce secondary metabolites attractive drug discovery due to their structural diversity and evolved interaction biomolecules. Here, we have established an extract library derived from isolated Vestfjorden, Norway, performed a screening campaign discover compounds. One activity was found...
Abstract The marine Arctic isolate Halomonas sp. R5-57 was sequenced as part of a bioprospecting project which aims to discover novel enzymes and organisms from low-temperature environments, with potential uses in biotechnological applications. Phenotypically, exhibits high salt tolerance over wide range temperatures has extra-cellular hydrolytic activities several substrates, indicating it secretes may function salinity conditions. Genome sequencing identified the genes involved...
During a research cruise in 2016, we isolated fungi from sediments, seawater, driftwood, fruiting bodies, and macroalgae using three different media to assess species richness potential bioactivity of cultivable marine the High Arctic region. Ten stations Svalbard archipelago (73–80 °N, 18–31 °E) were investigated 33 fungal isolates obtained. These grouped into 22 operational taxonomic units (OTUs) nuc rDNA internal transcribed spacer regions (ITS1-5.8S-ITS2 = ITS) with acut-off set at 98%...
We have investigated the biotechnological potential of Arctic marine bacteria for their ability to produce a broad spectrum cold‐active enzymes. Marine exhibiting these features are great interest both fundamental research and industrial applications. Macrobiota, water sediment samples been collected during 2010 2011 expeditions around Lofoten Svalbard islands. Bacteria were isolated from this material identified through 16S rRNA gene sequence analysis purpose establishing culture collection...
The crystal structure of the periplasmic/extracellular endonuclease I from Vibrio salmonicida has been solved to 1.5 Å resolution and, in comparison corresponding endonucleases V. cholerae and vulnificus, serves as a model system for investigation structural determinants involved temperature NaCl adaptation this enzyme class. overall fold three enzymes is essentially similar, but endonuclease displays significantly more positive surface potential than other two owing presence ten Lys...
l ‐2,4‐diaminobutyric acid (DABA) aminotransferases can catalyze the formation of amines at distal ω‐position substrates, and is intial rate‐limiting enzyme in biosynthesis pathway cytoprotecting molecule ( S )‐2‐methyl‐1,4,5,6‐tetrahydro‐4‐pyrimidine carboxylic (ectoine). Although there an industrial interest ectoine, DABA remain poorly characterized. Herein, we present crystal structure EctB (2.45 Å), a aminotransferase from Chromohalobacter salexigens DSM 3043, well‐studied organism with...
Cellulases play a key role in the enzymatic conversion of plant cell-wall polysaccharides into simple and economically relevant sugars. Thus, discovery novel cellulases from exotic biological niches is great interest as they may present properties that are valuable biorefining lignocellulosic biomass.
The family Teredinidae (shipworms) contains 70-plus species of boring bivalves specialized to live in and digest wood.Traditional means identification taxonomy this group encounter numerous challenges, often compounded by the diverse dynamic nature shipworm ecology distribution.Modern integrative taxonomic methods are shedding new light on complex group, from delineating cryptic resolving phylogenetic relationships within family.This study reported sequence data rafted western eastern...
The Tohoku tsunami of March 2011 ejected a vast amount debris into the Pacific Ocean.Wood boring shipworms (Bivalvia: Teredinidae) were either already present in, or settled on, wooden fraction this debris, offering unique opportunity to study shipworm diversity in rafted wood known origin and time ocean entry.Lumber other began appearing on Central (Hawaiian Islands) Eastern beaches 2013.Eighty pieces Japanese Tsunami Marine Debris (JTMD) consisting construction beams, trees, milled logs,...
Adaptation to extreme environments affects the stability and catalytic efficiency of enzymes, often endowing them with great industrial potential. We compared environmental adaptation secreted endonuclease I from cold-adapted marine fish pathogen Vibrio salmonicida (VsEndA) human cholerae (VcEndA). Kinetic analysis showed that VsEndA displayed unique halotolerance. It retained a considerable amount activity low concentrations at least 0.6 m NaCl, was adapted work higher salt than VcEndA by...
Resolving the enzymatic pathways leading to sialic acids (Sias) in bacteria are vitally important for understanding their roles pathogenesis and subsequent development of tools combat infections. A detailed characterization involved enzymes is also essential due highly applicable properties Sias, i.e., as used a wide range medical applications human nutrition. Bacterial strains that produce Sias display them mainly on cell surface mimic animal cells thereby evading host's immune system....
To characterize the chromosome-encoded metallo-β-lactamase (MBL) from psychrophilic, marine fish-pathogenic bacterium Aliivibrio salmonicida LFI1238 and check for presence of gene in other isolates both connected to fish-farming industry environment. The MBL was cloned intracellularly expressed Escherichia coli. Kinetic parameters, NaCl dependence, pH optimum temperature were determined using purified enzyme. VIM-2 enzyme a Pseudomonas aeruginosa hospital isolate used as counterpart...
The crystal structure of a periplasmic/extracellular endonuclease from Vibrio cholerae has been solved at low and neutral pH. Crystals grown pH 4.6 6.9 diffracted to 1.6 Å (on BM01A the ESRF) 1.95 rotating-anode generator), respectively. structures were compared with homologous enzyme in V. vulnificus. different values are essentially identical each other vulnificus enzyme. However, interesting features observed solvent structures. Both reveal presence chloride ion completely buried within...
The cold-adapted Rhodococcus sp. strain AW25M09 was isolated from an Atlantic hagfish caught off the shore of northern Norway as part ongoing bioprospecting project that aims to identify novel bacteria with biotechnological potential. Here, we present 5.8-Mb draft genome sequence, together details regarding origin and its sequence assembly.