A5071226120- SARS-CoV-2 and COVID-19 Research
- Extracellular vesicles in disease
- Viral gastroenteritis research and epidemiology
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- Growth and nutrition in plants
- Monoclonal and Polyclonal Antibodies Research
- Soybean genetics and cultivation
- Cell Adhesion Molecules Research
- Body Composition Measurement Techniques
- Animal Virus Infections Studies
- Environmental and biological studies
- Bacteriophages and microbial interactions
- COVID-19 Clinical Research Studies
- Protein purification and stability
- Muscle metabolism and nutrition
- Viral Infectious Diseases and Gene Expression in Insects
University of North Carolina at Chapel Hill
2023-2024
National Institutes of Health
2021-2022
National Institute of Allergy and Infectious Diseases
2021-2022
Universidad de la República
2010-2011
ABSTRACT Purifying extracellular vesicles (EVs) has been challenging because EVs are heterogeneous in cargo yet share similar sizes and densities. Most surface marker‐based affinity separation methods limited to research or diagnostic scales. We report that heparin chromatography can separate purified into two distinct subpopulations as ascertained by MS/MS: a non‐heparin‐binding (NHB) fraction contains classical EV markers such tetraspanins heparin‐binding (HB) enriched fibronectins...
ABSTRACT The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic caused the biggest public health crises in recent history. Many expect future introductions into human population. Hence, it is essential to understand basic biology of these viruses. In natural infection, SARS-CoV-2 Spike (S) glycoprotein co-expressed with all other viral proteins, which modify cellular compartments maximize virion assembly. By comparison, most S degraded when protein expressed isolation, as...
The spike (S) glycoprotein of the pandemic virus, SARS-CoV-2, is a critically important target vaccine design and therapeutic development. A high-yield, scalable, cGMP-compliant downstream process for stabilized, soluble, native-like S protein ectodomain necessary to meet extensive material requirements ongoing research As June 2021, proteins have exclusively been purified using difficult-to-scale, low-yield methodologies such as affinity size-exclusion chromatography. Herein we present...
Abstract High throughput process development (HTPD) using liquid handling robotics and RoboColumns is an established methodology in downstream to screen chromatography resins optimize designs meet target product profiles. However, HTPD not yet widely available for use viral clearance capability of the resin due a variety constraints. In present study, BSL‐1‐compatible, non‐infectious MVM model, MVM‐VLP, was tested assessment with various membrane operations mode. To detect MVM‐VLP high...
Abstract The spike (S) glycoprotein of the pandemic virus, SARS-CoV-2, is a critically important target vaccine design and therapeutic development. A high-yield, scalable, cGMP-compliant downstream process for stabilized, soluble, native-like S protein ectodomain necessary to meet extensive material requirements ongoing research As June 2021, proteins have exclusively been purified using difficult-to-scale, low-yield methodologies such as affinity size-exclusion chromatography. Herein we...