A5035320237- Extracellular vesicles in disease
- SARS-CoV-2 and COVID-19 Research
- Photoacoustic and Ultrasonic Imaging
- Digital Holography and Microscopy
- Cell Adhesion Molecules Research
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- COVID-19 Clinical Research Studies
- Nanopore and Nanochannel Transport Studies
University of North Carolina at Chapel Hill
2021-2024
Abstract Extracellular vesicles (EVs) are secreted from all cell types and intimately involved in tissue homeostasis. They being explored as vaccine gene therapy platforms, well potential biomarkers. As their size is below the diffraction limit of light microscopy, direct visualizations have been daunting single‐particle studies under physiological conditions hampered. Here, stochastic optical reconstruction microscopy (dSTORM) was employed to visualize EVs three‐dimensions localize molecule...
ABSTRACT Purifying extracellular vesicles (EVs) has been challenging because EVs are heterogeneous in cargo yet share similar sizes and densities. Most surface marker‐based affinity separation methods limited to research or diagnostic scales. We report that heparin chromatography can separate purified into two distinct subpopulations as ascertained by MS/MS: a non‐heparin‐binding (NHB) fraction contains classical EV markers such tetraspanins heparin‐binding (HB) enriched fibronectins...
ABSTRACT The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic caused the biggest public health crises in recent history. Many expect future introductions into human population. Hence, it is essential to understand basic biology of these viruses. In natural infection, SARS-CoV-2 Spike (S) glycoprotein co-expressed with all other viral proteins, which modify cellular compartments maximize virion assembly. By comparison, most S degraded when protein expressed isolation, as...
Extracellular vesicles (EVs) are released by all cell types and play an important role in signaling homeostasis. The visualization of EVs often require indirect methods due to their small diameter (40-250 nm), which is beneath the diffraction limit typical light microscopy. We have developed a super-resolution microscopy-based bypass both two three dimensions. Using this approach, we can resolve three-dimensional shape within +/- 20 nm resolution on XY-axis 50 along Z-axis. In conclusion,...
Extracellular vesicles (EVs) are released by all cell types and play an important role in signaling homeostasis. The visualization of EVs often require indirect methods due to their small diameter (40-250 nm), which is beneath the diffraction limit typical light microscopy. We have developed a super-resolution microscopy-based bypass both two three dimensions. Using this approach, we can resolve three-dimensional shape within +/- 20 nm resolution on XY-axis 50 along Z-axis. In conclusion,...