The main advantages of single-cell gel electrophoresis (SCG) are its applicability to any eukaryotic organism and cell type, low cost the short time required obtain results. These properties make SCG assay particularly useful in screening for environmental genotoxicity. present study describes a modified version this technique use field work with native rodents examines some factors which influence outcome assay. Wild (Ctenomys torquatus, "tuco-tuco") from region close strip coal mine no...

Coal is a mixture of variety chemicals, especially hydrocarbons, which may give rise to polycyclic aromatic hydrocarbons (PAH). Many PAH compounds produce mutagenic and carcinogenic effects. The quality mineral coal in Rio Grande do Sul (RS) low it typically obtained by stripping operations; represents approximately 87% the Brazil reserves. This report concerns application Comet assay Ctenomys torquatus detect effects coal, comparing results with micronucleus (MN) assay, both using...

Species identification through DNA barcoding is a tool to be added taxonomic procedures, once it has been validated. Applying techniques in public health would aid the and correct delimitation of distribution rodents from subfamily Sigmodontinae. These are reservoirs etiological agents zoonoses including arenaviruses, hantaviruses, Chagas disease leishmaniasis. In this study we compared distance-based probabilistic phylogenetic inference methods evaluate performance cytochrome c oxidase...

Abstract We investigated the demographic parameters and relationships between population density extrinsic factors (temperature, rainfall, humidity insolation) of two sigmodontine rodents (

The wildlife of the Brazilian Pampa is threatened by large-scale habitat loss, due in particular to expansion soybean cultivation and conversion grasslands areas into extensive silviculture. It essential study how mammal fauna copes with highly fragmented, human-influenced, non-protected landscape. Our presents results a survey large- medium-sized mammals typical human-influenced steppic savanna area biome. was conducted exclusively use camera traps over period 16 months. relative...

The population genetic structure of two sympatric species sigmodontine rodents (Oligoryzomys nigripes and Euryoryzomys russatus) was examined for mitochondrial DNA (mtDNA) sequence haplotypes the control region. Samples were taken from three localities in Atlantic Rain Forest southern Brazil, along an altitudinal gradient with different types habitat. In both there no throughout their distribution, although levels variability gene flow high.

Fragmentation and destruction of a habitat are strongly relevant aspects to determine the richness dynamics mammals in ecosystems. This study, developed from October, 2010 July, 2011 three Atlantic Forest remnants Ipumirim, SC, Brazil, aims at identifying diversity large- medium-sized nonflying verifying associations patterns obtained with features researched areas. The approximate measurement inventoried areas is 51 ha. data collection mammal fauna was through direct registers, use...

Small land mammals possess features that significantly influence the dynamics of ecosystems and participate in various levels food web. In Brazilian Atlantic Forest richness these animals is high, which makes them even more ecologically numerically relevant this environment. context, we investigated species composition small an unexplored area southern Brazil, compared with other regions order to understand how community related others. The study was located interior forest formation, at a...

The geographic distribution of Calomys laucha in Brazil, known only from the southern portion Rio Grande do Sul state, up to state’s Central Depression region, is extended approximately 350 km north with a new record at Faxinalzinho municipality, post region state. species seems be associated dense shrubby vegetation this area.

We recorded three individuals of Abrawayaomys ruschii Cunha & Cruz, 1979 in Chapecó, Santa Catarina state, expanding the known geographic distribution species approximately 370 km west from its nearest locality. It is second record this type physiognomy, characterized by transition Seasonal Deciduous and Mixed Ombrophilous forests. Additionally, closest record, about 200 east, recently described chebezi Pardiñas, Teta D’Elía, 2009.

The abundance of small mammal species is particularly influenced by vegetation and arthropods. Studies that focus on understanding these correlations between mammals, habitat features resource availability will provide insights into the ecological processes drive communities. We investigated terrestrial arthropod taxa trees treelets with composition a community in patch Araucaria rain forest southern Brazil. established 25 stratified plots sampling grid mammals sampled abundances trees,...

e13039 Background: Oral and pharyngeal cancer is the 11th most common malignancy worldwide. Although oral cavity a potentially accessible site for examination, up to 50% of cancers are detected at advanced stage. The Mobile Unit Stomatology Dental Medicine (UMEMD) Portuguese League Against Cancer (LPCC) Association Hospital (APMDH) has developed early diagnosis consultations. This consultations were performed by team 50 doctors specialized in diagnosis. Once month UMEMD went different region...

Among the sigmodontine rodents, genus Akodon is second in numerical terms, with about 45 recognized species. In Rio Grande do Sul state, there are 5 registered This article shows data on distribution of species northern as well a description karyotypes from this region shown. Six localities were sampled August 2008 to May 2010. A total 44 specimens underwent cytogenetic analysis and 2 identified (Akodon montensis paranaensis). The karyotype A. was 2n = 24, FN that paranaensis 44, NF 46. Data...

Abstract The principle and the techniques applied in DNA extraction play a pivotal role obtention of purified genetic material. present study investigates efficiency eight protocols Hypostomus commersoni , an essential component South American freshwater ichthyofauna. quality samples was assessed through spectrophotometry, gel electrophoresis, PCR-RAPD amplification. influenced both by method target-tissue choice. Higher concentrations yield were obtained from ocular tissue, with positive...