A5000956791- Genetics, Aging, and Longevity in Model Organisms
- Microtubule and mitosis dynamics
- Photosynthetic Processes and Mechanisms
- Fungal and yeast genetics research
- Cardiomyopathy and Myosin Studies
- Hippo pathway signaling and YAP/TAZ
- Algal biology and biofuel production
- Spaceflight effects on biology
- RNA Research and Splicing
- Genetic Neurodegenerative Diseases
- Cellular Mechanics and Interactions
- Cellular transport and secretion
- Mitochondrial Function and Pathology
- Circadian rhythm and melatonin
- 14-3-3 protein interactions
- Reproductive Biology and Fertility
- Cell Adhesion Molecules Research
- Pluripotent Stem Cells Research
- Cell Image Analysis Techniques
- ATP Synthase and ATPases Research
- Protist diversity and phylogeny
- Toxin Mechanisms and Immunotoxins
- Plant Molecular Biology Research
- Advanced Memory and Neural Computing
- Kruppel-like factors research
National University of Singapore
2013-2024
Temasek Life Sciences Laboratory
2013-2024
Hokkaido University
2021-2024
Howard Hughes Medical Institute
2007-2011
Johns Hopkins University
2007-2011
RIKEN Center for Biosystems Dynamics Research
2006-2010
The University of Tokyo
1997-2004
Tokyo University of the Arts
2004
National Institute for Basic Biology
2002
Chromosome segregation requires stable bipolar attachments of spindle microtubules to kinetochores. The dynein/dynactin motor complex localizes transiently kinetochores and is implicated in chromosome segregation, but its role remains poorly understood. Here, we use the Caenorhabditis elegans embryo investigate function kinetochore dynein by analyzing Rod/Zwilch/Zw10 (RZZ) associated coiled-coil protein SPDL-1. Both components are essential for Mad2 targeting checkpoint activation. RZZ...
Asymmetric segregation of P granules during the first four divisions Caenorhabditis elegans embryo is a classic example cytoplasmic partitioning germline determinants. It thought that asymmetric granule components mitosis essential to distinguish from soma. We have identified mutant (pptr-1) in which become unstable and proteins RNAs are distributed equally somatic blastomeres. Despite symmetric components, pptr-1 mutants segregate uniquely expresses postembryonic development. fertile,...
Polarization of the C. elegans zygote is initiated by ECT-2-dependent cortical flows, which mobilize anterior PAR proteins (PAR-3, PAR-6 and PKC-3) away from future posterior end embryo marked sperm centrosome. Here, we demonstrate existence a second, parallel redundant pathway that can polarize in absence flows. This second depends on polarity protein PAR-2. We show PAR-2 localizes to cortex nearest centrosome even Once cortex, antagonizes PAR-3-dependent recruitment myosin, creating myosin...
During cytokinesis, a signal from the central spindle that forms between separating anaphase chromosomes promotes accumulation of contractile ring components at cell equator, while centrosomal microtubule asters inhibits poles. However, molecular identity inhibitory has remained unknown. To identify aster-based signal, we developed means to monitor removal proteins polar cortex after onset. Using this assay, show clearing is an active process requires activation Aurora A kinase by TPXL-1....
Cytokinesis in many eukaryotes requires an actomyosin contractile ring. Here, we show that fission yeast the myosin-II heavy chain Myo2 initially accumulates at division site via its COOH-terminal 134 amino acids independently of F-actin. The region can access to early G2, whereas intact does so mitosis. Ser1444 is a phosphorylation dephosphorylated during S1444A prematurely future and promotes formation F-actin ring even interphase. accumulation anillin homologue Mid1 functions proper...
ABSTRACT Schizosaccharomyces pombe cells divide by virtue of the F-actin-based contractile ring (F-actin ring). Two myosin-II heavy chains, Myo2 and Myp2/Myo3, have been localized to F-actin ring. Here, we investigated mechanism assembly at division site in S. cells. First, showed that Cdc4, an EF-hand protein, appears be a common myosin light chain associated with both Myo3. Loss function Myo3 caused defect site, like phenotype cdc4 null It is suggested Myo2, Cdc4 cooperative manner...
ABSTRACT In order to identify additional components important for cell division in the fission yeast Schizosaccharomyces pombe we have screened a bank of conditional cold-sensitive mutants cytokinesis defects. One these showed delay cleavage, and strong genetic interactions with other genes implicated medial ring formation. Cloning corresponding gene indicates that it encodes protein significant homology regulatory light chain non-muscle myosins. We named rlc1 (regulatory 1). The is not...
We characterized the novel Schizosaccharomyces pombegenes myo4 + andmyo5 , both of which encode myosin-V heavy chains. Disruption caused a defect in cell growth and led to an abnormal accumulation secretory vesicles throughout cytoplasm. The mutant cells were rounder than normal, although sites for polarization still established. Elongation ends completion septation required more time wild-type cells, indicating that Myo4 functions polarized at during septation. Consistent with this...
We cloned the myo3 + gene of Schizosaccharomyces pombe which encodes a type‐II myosin heavy chain. null cells showed defect in cytokinesis under certain conditions. Overproduction Myo3 also cytokinesis. Double mutant analysis indicated that genetically interacts with Cdc8 tropomyosin and actin. may be implicated stabilization F‐actin cables. Moreover, function Myo2 can replaced by overexpressed Myo3. observed modest synthetic interaction between Thus, seem to cooperate formation ring S. .
Cell polarity arises through the spatial segregation of regulators. PAR proteins are regulators that localize asymmetrically to two opposing cortical domains. However, it is unclear how spatially segregated interact maintain their mutually exclusive partitioning. Here, single-molecule detection analysis in Caenorhabditis elegans embryos reveals PAR-2 diffuses only short distances, and, as a result, most molecules associate and dissociate from cortex without crossing into domain. Our results...
Background Type I myosin is highly conserved among eukaryotes, and apparently plays important roles in a number of cellular processes. In the budding yeast, two species have been identified their role F‐actin assembly has inferred. Results We cloned fission yeast myo1 gene, which encoded protein. Disruption was not lethal, but it caused growth retardation at high low temperatures, sensitivity to concentration KCl, aberrance cell morphology associated with an abnormal distribution patches. An...
Contact inhibition of proliferation is a critical cell density control mechanism governed by the Hippo signalling pathway. The biochemical underlying density‐dependent cues regulating and its downstream effectors, YAP, remains poorly understood. Here, we reveal that tight junction protein ZO‐2 required for contact‐mediated proliferation. We additionally determined well‐established molecular players this process, namely kinase LATS1 are regulated scaffolding function promotes interaction with...
Coordination between cell differentiation and proliferation during development requires the balance asymmetric symmetric modes of division. However, cellular intrinsic cue underlying choice these two division remains elusive. Here, we show evidence in Caenorhabditis elegans that invariable lineage is specified by antagonizing complexes partitioning-defective (PAR) proteins. By uncoupling unequal inheritance PAR proteins from fate determinants division, demonstrate changes PAR-2 PAR-6 can be...
Background Elongation factor 1α (EF1α), an essential component of the eukaryotic translational machinery, has been shown to possess various biochemical and biological activities, including F‐actin‐binding ‐bundling, microtubule‐ severing, activity making fibroblasts highly susceptible transformation. However, our understanding significance EF1α with respect these or activities remains limited. Here we report identification EF1α‐encoding genes as whose over‐expression causes aberrant cell...