A5058214031- Transplantation: Methods and Outcomes
- Renal Transplantation Outcomes and Treatments
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Polyomavirus and related diseases
- Immunotherapy and Immune Responses
- Pluripotent Stem Cells Research
- Acute Lymphoblastic Leukemia research
- Organ Transplantation Techniques and Outcomes
- Hematopoietic Stem Cell Transplantation
- CRISPR and Genetic Engineering
- Biomedical Ethics and Regulation
- Folate and B Vitamins Research
- Reproductive System and Pregnancy
- Rheumatoid Arthritis Research and Therapies
- Hepatitis B Virus Studies
- Antimicrobial Resistance in Staphylococcus
- Spondyloarthritis Studies and Treatments
- Organ Donation and Transplantation
- Monoclonal and Polyclonal Antibodies Research
- Autoimmune and Inflammatory Disorders Research
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- GDF15 and Related Biomarkers
- Cytomegalovirus and herpesvirus research
- Artificial Intelligence in Healthcare
Australian Red Cross Lifeblood
2010-2024
Medical College of Wisconsin
2019
Services Australia
2010-2018
Donor selection in lung transplantation (LTx) is historically based upon clinical urgency, ABO compatibility, and donor size. HLA matching not routinely considered; however, the presence or later development of anti-HLA antibodies associated with poorer outcomes, particularly chronic allograft dysfunction (CLAD). Using eplet mismatches, we aimed to determine whether donor/recipient incompatibility was a significant predictor CLAD. One hundred seventy-five LTx undertaken at Alfred Hospital...
HLA (HLA) are a major barrier to transplant success, as HLA‐A and ‐B molecules principal ligands for T‐cells, HLA‐C Killer cell Immunoglobulin‐like Receptors (KIR), directing Natural (NK) function. designated “C1” or “C2” based on residues 77 80, which determine the NK responses. Here, we investigated donor/recipient mismatch associations with development of chronic lung allograft dysfunction (CLAD) following transplantation (LTx). 310 LTx pairs were Next Generation Sequenced assessed C1 C2...
Abstract We aim to create a bank of clinical grade cord blood-derived induced pluripotent stem cell lines in order facilitate research leading the development new cellular therapies. Here we present clear pathway toward creation such resource, within strong quality framework, and with appropriate regulatory, government ethics approvals, along dynamic follow-up re-consent process blood donors from public BMDI Cord Blood Bank. Interrogation inventory next generation sequencing was used...
Abstract Background : Reactive arthritis (ReA) is an inflammatory triggered by certain gastrointestinal and genitourinary infections. Single source outbreaks of triggering infections provide opportunity to elucidate host susceptibility factors in this disease. Aim To determine the role Major Histocompatibility Complex (MHC) Class I alleles ReA after two large single Salmonella Typhimurium gastroenteritis. Methods A questionnaire screening for features a request HLA class typing were sent all...
This study examined the contribution single nucleotide polymorphisms (SNPs) of 5,10-methylenetetrahydrofolate reductase (MTHFR) gene have on clinical outcomes in hematopoietic stem cell transplant patients treated with antiproliferative drug methotrexate. Two common SNPs, 677C>T and 1298A>C, were genotyped by polymerase chain reaction-restriction fragment-length polymorphism (PCR-RFLP) from samples obtained patient DNA samples. Eleven including survival graft-versus-host disease (GVHD)...
Background. Currently, the assessment of immunological risk in lung transplantation (LTx) does not completely consider HLA compatibility at molecular level. We have previously demonstrated association eplets predicting chronic allograft dysfunction following LTx; however, associations between eplet mismatch (epMM) loads and overall survival are unknown. Methods. In this retrospective, single-center study, 277 LTx donor-recipient pairs were high resolution typed analyzed for epMMs using...
Immune sensitization, defined as the presence of alloreactive donor-specific antibodies (DSA), is associated with increased wait-times and inferior transplant outcomes. Identifying pretransplant DSA a physical cell-based assay critical in defining immunological risk. However, improved solid phase antibody detection has provided potential to forgo this assay. Here, we evaluated association between mean fluorescence intensity (MFI) recently introduced Halifaster Flow cytometry crossmatch (FXM)...
Waitlisted sensitised transplant recipients with HLA allele level antibodies to their own antigen family are disadvantaged by current deficiencies in typing for deceased donors. This is primarily because at time of organ allocation, provided whereas solid phase assays provide antibody definition. The gold standard next generation sequencing (NGS), however limitations established NGS systems prevent use Instead, many labs a real-time PCR (qPCR) result donors, which can disadvantage patients....
The human leucocyte antigens ( HLA ) genes are part of the major histocompatibility complex and most polymorphic gene in genome. hyperpolymorphic, that is many forms (alleles) at each locus. molecules play a critical role our immune defence, extreme diversity ensures robust response. Over generations, types retained through advantage which may be basis high frequency some alleles specific regions. Geographical, language or religious influences can further influence regional allele frequency....